ID PTUCB_FUSMR Reviewed; 526 AA.
AC O06900;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 2.
DT 03-APR-2013, entry version 89.
DE RecName: Full=PTS system alpha-glucoside-specific EIICB component;
DE Includes:
DE RecName: Full=Alpha-glucoside permease IIC component;
DE AltName: Full=PTS system alpha-glucoside-specific EIIC component;
DE Includes:
DE RecName: Full=Alpha-glucoside-specific phosphotransferase enzyme IIB component;
DE EC=2.7.1.69;
DE AltName: Full=PTS system alpha-glucoside-specific EIIB component;
GN Name=malB;
OS Fusobacterium mortiferum.
OC Bacteria; Fusobacteria; Fusobacteriales; Fusobacteriaceae;
OC Fusobacterium.
OX NCBI_TaxID=850;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 25557 / CCUG 14475;
RX PubMed=11882720;
RA Pikis A., Immel S., Robrish S.A., Thompson J.;
RT "Metabolism of sucrose and its five isomers by Fusobacterium
RT mortiferum.";
RL Microbiology 148:843-852(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 443-526.
RC STRAIN=ATCC 25557 / CCUG 14475;
RX PubMed=9209025;
RA Bouma C.L., Reizer J., Reizer A., Robrish S.A., Thompson J.;
RT "6-phospho-alpha-D-glucosidase from Fusobacterium mortiferum: cloning,
RT expression, and assignment to family 4 of the glycosylhydrolases.";
RL J. Bacteriol. 179:4129-4137(1997).
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar
CC phosphotransferase system (sugar PTS), a major carbohydrate active
CC -transport system, catalyzes the phosphorylation of incoming sugar
CC substrates concomitantly with their translocation across the cell
CC membrane. This system is involved in alpha-glucoside transport.
CC -!- CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L-
CC histidine/cysteine + sugar = protein EIIB + sugar phosphate.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein
CC (Probable).
CC -!- DOMAIN: The EIIC domain forms the PTS system translocation channel
CC and contains the specific substrate-binding site.
CC -!- DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a
CC cysteinyl or histidyl residue, depending on the transported sugar.
CC Then, it transfers the phosphoryl group to the sugar substrate
CC concomitantly with the sugar uptake processed by the EIIC domain.
CC -!- SIMILARITY: Contains 1 PTS EIIB type-1 domain.
CC -!- SIMILARITY: Contains 1 PTS EIIC type-1 domain.
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DR EMBL; U81185; AAB63014.2; -; Genomic_DNA.
DR ProteinModelPortal; O06900; -.
DR TCDB; 4.A.1.1.4; PTS glucose-glucoside (Glc) family.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:EC.
DR GO; GO:0005351; F:sugar:hydrogen symporter activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR Gene3D; 3.30.1360.60; -; 1.
DR InterPro; IPR018113; PTrfase_EIIB/Cys_phosph_CS.
DR InterPro; IPR001996; PTS_EIIB_1.
DR InterPro; IPR003352; PTS_EIIC.
DR InterPro; IPR013013; PTS_EIIC_1.
DR InterPro; IPR011535; PTS_Glc-like_IIB_component.
DR InterPro; IPR010975; PTS_IIBC_a_glc.
DR Pfam; PF00367; PTS_EIIB; 1.
DR Pfam; PF02378; PTS_EIIC; 1.
DR SUPFAM; SSF55604; PTS_EIIB; 1.
DR TIGRFAMs; TIGR00826; EIIB_glc; 1.
DR TIGRFAMs; TIGR02005; PTS-IIBC-alpha; 1.
DR PROSITE; PS51098; PTS_EIIB_TYPE_1; 1.
DR PROSITE; PS01035; PTS_EIIB_TYPE_1_CYS; 1.
DR PROSITE; PS51103; PTS_EIIC_TYPE_1; 1.
PE 4: Predicted;
KW Cell membrane; Kinase; Membrane; Phosphotransferase system;
KW Sugar transport; Transferase; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1 526 PTS system alpha-glucoside-specific EIICB
FT component.
FT /FTId=PRO_0000186477.
FT TRANSMEM 12 32 Helical; (Potential).
FT TRANSMEM 59 79 Helical; (Potential).
FT TRANSMEM 88 108 Helical; (Potential).
FT TRANSMEM 132 152 Helical; (Potential).
FT TRANSMEM 173 193 Helical; (Potential).
FT TRANSMEM 200 220 Helical; (Potential).
FT TRANSMEM 224 244 Helical; (Potential).
FT TRANSMEM 274 294 Helical; (Potential).
FT TRANSMEM 305 325 Helical; (Potential).
FT TRANSMEM 330 350 Helical; (Potential).
FT TRANSMEM 355 375 Helical; (Potential).
FT TRANSMEM 381 401 Helical; (Potential).
FT DOMAIN 1 417 PTS EIIC type-1.
FT DOMAIN 447 526 PTS EIIB type-1.
FT ACT_SITE 469 469 Phosphocysteine intermediate; for EIIB
FT activity (By similarity).
SQ SEQUENCE 526 AA; 57313 MW; E5FDE7287202D84E CRC64;
MLKHFQRLGG ALFAPVLLFP FAGLVVALTI ILKNPDFVGE LANTNGTFYK MITVIEEGGW
TVFRQLPLIF AIGLPIGLAK KAHPRACLAV LATYLTYNYF ISAILTFWGP SFGVDFTQNV
GGVSGLTTIA GIKTLDTSIV GAIVISGITI YIHNKFFDTK LPDFLGTFQG TTLVSAIAFV
VMIPCAYITC LVWPKIQMGI SSLQALMVTS GTFGVWLYTF LERILIPTGL HHFIYGPFIF
GPAVVDTGIQ VAWAENLLNF ANSTQPLKEL FPQGGFALHG NSKIFGCIGI ALAMYKTARP
EKKKIVSGLL IPAALTAALV GITEPLEFTF LFIAPFLFVV HAVLAATMAA VMYAFGVVKY
GSGIIEIAAL NWLPLMKNHS GVMFTQLAIG VVFIGIHYLV FKFLIEKYNV KTSGREDEEE
ETKLYTKADW KAKNGEGKET NSSDLYSGKA KAFLEAFGGK DNIEQVNNCA TRLRISVKDE
KKVGPDIQFK AAGAHGVVRN GKAFQVIVGL SVPQVRESFE NLMEQN
//
