ID O07179_MYCTU Unreviewed; 304 AA.
AC O07179; F2GMH4; I6Y3T8; Q7D9S5;
DT 01-JUL-1997, integrated into UniProtKB/TrEMBL.
DT 01-AUG-1998, sequence version 2.
DT 27-MAR-2024, entry version 152.
DE SubName: Full=Enoyl-CoA hydratase EchA2 (Enoyl hydrase) (Unsaturated acyl-CoA hydratase) (Crotonase) {ECO:0000313|EMBL:CCP43187.1};
GN Name=echA2 {ECO:0000313|EMBL:CCP43187.1};
GN OrderedLocusNames=Rv0456c {ECO:0000313|EMBL:CCP43187.1};
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332 {ECO:0000313|EMBL:CCP43187.1, ECO:0000313|Proteomes:UP000001584};
RN [1] {ECO:0000313|EMBL:CCP43187.1, ECO:0000313|Proteomes:UP000001584}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv {ECO:0000313|Proteomes:UP000001584};
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C., Harris D.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E.III., Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L., Oliver K., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton J.,
RA Squares R., Squares S., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2] {ECO:0007829|PubMed:21969609}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21969609; DOI=10.1074/mcp.M111.011445;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Could possibly oxidize fatty acids using specific components.
CC {ECO:0000256|ARBA:ARBA00002994}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:58856; EC=4.2.1.17;
CC Evidence={ECO:0000256|ARBA:ARBA00023709};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O;
CC Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521,
CC ChEBI:CHEBI:137480; EC=4.2.1.17;
CC Evidence={ECO:0000256|ARBA:ARBA00023717};
CC -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC {ECO:0000256|ARBA:ARBA00005254, ECO:0000256|RuleBase:RU003707}.
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DR EMBL; AL123456; CCP43187.1; -; Genomic_DNA.
DR RefSeq; NP_214970.1; NC_000962.3.
DR RefSeq; WP_003898463.1; NZ_NVQJ01000002.1.
DR AlphaFoldDB; O07179; -.
DR SMR; O07179; -.
DR STRING; 83332.Rv0456c; -.
DR PaxDb; 83332-Rv0456c; -.
DR DNASU; 886312; -.
DR GeneID; 886312; -.
DR KEGG; mtu:Rv0456c; -.
DR PATRIC; fig|83332.111.peg.499; -.
DR TubercuList; Rv0456c; -.
DR eggNOG; COG1024; Bacteria.
DR InParanoid; O07179; -.
DR OrthoDB; 3206737at2; -.
DR PhylomeDB; O07179; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0003677; F:DNA binding; IDA:MTBBASE.
DR GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0045734; P:regulation of acetate catabolic process; IDA:MTBBASE.
DR CDD; cd06558; crotonase-like; 1.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR PANTHER; PTHR43802; ENOYL-COA HYDRATASE; 1.
DR PANTHER; PTHR43802:SF1; IP11341P-RELATED; 1.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
DR PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1.
PE 1: Evidence at protein level;
KW Reference proteome {ECO:0000313|Proteomes:UP000001584}.
FT REGION 279..304
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 304 AA; 33086 MW; 70F66FF5F339725A CRC64;
MPTPDFQTLL YTTAGPVATI TLNRPEQLNT IVPPMPDEIE AAIGLAERDQ DIKVIVLRGA
GRAFSGGYDF GGGFQHWGDA MMTDGRWDPG KDFAMVTARE TGPTQKFMAI WRASKPVIAQ
VHGWCVGGAS DYALCADIVI ASEDAVIGTP YSRMWGAYLT GMWLYRLSLA KVKWHSLTGR
PLTGVQAAEA ELINEAVPFE RLEARVAEIA TELARIPLSQ LQAQKLIVNQ AYENMGLAST
QLLGGILDGL MRNTPDALEF IRTAQTQGVR AAVERRDGPF GDYSQAPPEL RPDPTHVITP
DGSM
//
