ID O13098_XENLA Unreviewed; 897 AA.
AC O13098;
DT 01-JUL-1997, integrated into UniProtKB/TrEMBL.
DT 01-JUL-1997, sequence version 1.
DT 27-MAR-2024, entry version 118.
DE RecName: Full=Neurofilament medium polypeptide {ECO:0000256|ARBA:ARBA00015894};
DE AltName: Full=160 kDa neurofilament protein {ECO:0000256|ARBA:ARBA00030258};
DE AltName: Full=Neurofilament triplet M protein {ECO:0000256|ARBA:ARBA00032778};
GN Name=nefm.L {ECO:0000313|RefSeq:NP_001081679.1,
GN ECO:0000313|Xenbase:XB-GENE-6252137};
GN Synonyms=nef-m {ECO:0000313|RefSeq:NP_001081679.1}, nef3
GN {ECO:0000313|RefSeq:NP_001081679.1}, nefm
GN {ECO:0000313|RefSeq:NP_001081679.1,
GN ECO:0000313|Xenbase:XB-GENE-6252137}, nefm-a
GN {ECO:0000313|RefSeq:NP_001081679.1}, nefm-b
GN {ECO:0000313|RefSeq:NP_001081679.1}, nf-m
GN {ECO:0000313|RefSeq:NP_001081679.1};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355 {ECO:0000313|EMBL:AAB53389.1};
RN [1] {ECO:0000313|EMBL:AAB53389.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=9332720; DOI=10.1016/S0169-328X(97)00096-X;
RA Gervasi C., Szaro B.G.;
RT "Sequence and expression patterns of two forms of the middle molecular
RT weight neurofilament protein (NF-M) of Xenopus laevis.";
RL Brain Res. Mol. Brain Res. 48:229-242(1997).
RN [2] {ECO:0000313|RefSeq:NP_001081679.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=12454917; DOI=10.1002/dvdy.10174;
RA Klein S.L., Strausberg R.L., Wagner L., Pontius J., Clifton S.W.,
RA Richardson P.;
RT "Genetic and genomic tools for Xenopus research: The NIH Xenopus
RT initiative.";
RL Dev. Dyn. 225:384-391(2002).
RN [3] {ECO:0000313|RefSeq:NP_001081679.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SIMILARITY: Belongs to the intermediate filament family.
CC {ECO:0000256|RuleBase:RU000685}.
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DR EMBL; U85969; AAB53389.1; -; mRNA.
DR PIR; A60094; A60094.
DR RefSeq; NP_001081679.1; NM_001088210.1.
DR DNASU; 397994; -.
DR GeneID; 397994; -.
DR KEGG; xla:397994; -.
DR AGR; Xenbase:XB-GENE-6252137; -.
DR CTD; 397994; -.
DR Xenbase; XB-GENE-6252137; nefm.L.
DR OrthoDB; 4640531at2759; -.
DR Proteomes; UP000186698; Chromosome 3L.
DR Bgee; 397994; Expressed in brain and 4 other cell types or tissues.
DR GO; GO:0005882; C:intermediate filament; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR Gene3D; 1.20.5.170; -; 1.
DR Gene3D; 1.20.5.500; Single helix bin; 1.
DR Gene3D; 1.20.5.1160; Vasodilator-stimulated phosphoprotein; 1.
DR InterPro; IPR018039; IF_conserved.
DR InterPro; IPR039008; IF_rod_dom.
DR InterPro; IPR006821; Intermed_filament_DNA-bd.
DR InterPro; IPR002957; Keratin_I.
DR PANTHER; PTHR45652; GLIAL FIBRILLARY ACIDIC PROTEIN; 1.
DR PANTHER; PTHR45652:SF3; NEUROFILAMENT MEDIUM POLYPEPTIDE; 1.
DR Pfam; PF00038; Filament; 1.
DR Pfam; PF04732; Filament_head; 1.
DR PRINTS; PR01248; TYPE1KERATIN.
DR SMART; SM01391; Filament; 1.
DR SUPFAM; SSF64593; Intermediate filament protein, coiled coil region; 2.
DR SUPFAM; SSF90257; Myosin rod fragments; 1.
DR PROSITE; PS00226; IF_ROD_1; 1.
DR PROSITE; PS51842; IF_ROD_2; 1.
PE 2: Evidence at transcript level;
KW Coiled coil {ECO:0000256|ARBA:ARBA00023054, ECO:0000256|SAM:Coils};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Intermediate filament {ECO:0000256|ARBA:ARBA00022754,
KW ECO:0000256|RuleBase:RU000685};
KW Reference proteome {ECO:0000313|Proteomes:UP000186698}.
FT DOMAIN 86..397
FT /note="IF rod"
FT /evidence="ECO:0000259|PROSITE:PS51842"
FT REGION 1..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 497..835
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 97..229
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 281..368
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 508..655
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 656..682
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 683..747
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 772..795
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 808..827
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 897 AA; 100685 MW; D96221D9606DB077 CRC64;
MSYSLETIGN PSYRRMAETR SSYSRTSSSP SSGFRSQSWS RSSPTTISSY KRTNLAAPRV
YSSTDSLDLS QSSAFNGGLK QVRFSEKEQL QGLNDRFAGY IEKVHFLEQQ NKEIEAEIQA
LRQKHSGYSQ LGEVYEQEMR ELRANLELLG HDKAQIVLDS EHLEEDLQRL KERLDEEARI
RDETEAAIRA MKKDMDDTSL VKVELDKKVQ SLQEEVNFIK NNHEEEVGEL LAQIQASSIT
VERKDFQKTD LASALREIRS QLEGHSSQNM IQTEEVFRNR YAKLTEAAEQ NKDAIRSAKD
EIAEYRRQLH SKTVELESVR GTKESLERQL NDIEERHGHD LTSYQETIQQ LDNELRGTKW
EMSRHLREYQ DLLNVKMALD IEIAAYRKLL EGEETRFCSF SGVPTGPSAI HRPAPIIVSS
TRVQHARSEP PRMKVQHKFV EEIIEETKVE DDRSDMEAAL AAMADEFASG LLDGGEEEEQ
EEETQEGGEA EEEIVAAVES SVQAAAPGEE GEEEEEEKGE DEAEAVEEGE KEEEEEEKGE
EGEEEGGEQG EEEDVKADEK EEGEEEAEGE EAKTEEGEEE GETVEEEAEK EEGGEEEEKE
EVAAEEEGGE GEEEGGEGET EEDKGEVEKE EEAETEEAEG GDEEATEEAQ DEEVVEETMT
ETKIVREKAE VQEKAAEGEE KQDSEEQKDE GEDEETGEGK EEAEEKEEGG EEKEDASEGT
EQQEEEKAEE EKGESEEGAE EDAGEDEQAK SPEEKSSEET ISVSKAASVG PEKEGAKQEE
EKSTQRDNGK EDTAVNGDVE EEEVKEESED KAVVTNGLDD SPSEDKESSK GQQTVVITKK
IETTTSEGED GTKYITKSVT VTQKVEEHEE TVQEKTVSTK KVEKVTSHAV KEVTEDK
//
