ID CUX2_HUMAN Reviewed; 1486 AA.
AC O14529; A7E2Y4;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 4.
DT 01-MAY-2013, entry version 123.
DE RecName: Full=Homeobox protein cut-like 2;
DE AltName: Full=Homeobox protein cux-2;
GN Name=CUX2; Synonyms=CUTL2, KIAA0293;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT LEU-1472.
RC TISSUE=Brain;
RX PubMed=9179496; DOI=10.1093/dnares/4.1.53;
RA Ohara O., Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N.,
RA Nomura N.;
RT "Construction and characterization of human brain cDNA libraries
RT suitable for analysis of cDNA clones encoding relatively large
RT proteins.";
RL DNA Res. 4:53-59(1997).
RN [2]
RP SEQUENCE REVISION.
RX PubMed=12168954; DOI=10.1093/dnares/9.3.99;
RA Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.;
RT "Construction of expression-ready cDNA clones for KIAA genes: manual
RT curation of 330 KIAA cDNA clones.";
RL DNA Res. 9:99-106(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16541075; DOI=10.1038/nature04569;
RA Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M.,
RA Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B.,
RA Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D.,
RA Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z.,
RA Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z.,
RA Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H.,
RA Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H.,
RA Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V.,
RA Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J.,
RA Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A.,
RA Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M.,
RA Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E.,
RA Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M.,
RA Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R.,
RA Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J.,
RA Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C.,
RA Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M.,
RA Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M.,
RA Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P.,
RA Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L.,
RA Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E.,
RA Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C.,
RA Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F.,
RA Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M.,
RA Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S.,
RA Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D.,
RA Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I.,
RA Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T.,
RA Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S.,
RA Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D.,
RA Kucherlapati R., Weinstock G., Gibbs R.A.;
RT "The finished DNA sequence of human chromosome 12.";
RL Nature 440:346-351(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT LEU-1472.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, AND MASS
RP SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [6]
RP STRUCTURE BY NMR OF 544-631 AND 887-1125.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the first, second and third CUT domains of
RT human homeobox protein CUX-2 (CUT-like 2).";
RL Submitted (OCT-2005) to the PDB data bank.
CC -!- FUNCTION: May be a transcription factor involved in neural
CC specification. Binds to DNA in a sequence-specific manner (By
CC similarity).
CC -!- SUBCELLULAR LOCATION: Nucleus (By similarity).
CC -!- SIMILARITY: Belongs to the CUT homeobox family.
CC -!- SIMILARITY: Contains 3 CUT DNA-binding domains.
CC -!- SIMILARITY: Contains 1 homeobox DNA-binding domain.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA22962.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;
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DR EMBL; AB006631; BAA22962.2; ALT_INIT; mRNA.
DR EMBL; AC005805; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC002979; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC002352; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC002978; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC151245; AAI51246.1; -; mRNA.
DR IPI; IPI00022370; -.
DR RefSeq; NP_056082.2; NM_015267.3.
DR UniGene; Hs.124953; -.
DR PDB; 1WH6; NMR; -; A=887-974.
DR PDB; 1WH8; NMR; -; A=1028-1125.
DR PDB; 1X2L; NMR; -; A=544-631.
DR PDBsum; 1WH6; -.
DR PDBsum; 1WH8; -.
DR PDBsum; 1X2L; -.
DR ProteinModelPortal; O14529; -.
DR SMR; O14529; 549-631, 887-976, 1032-1220.
DR STRING; 9606.ENSP00000261726; -.
DR PhosphoSite; O14529; -.
DR PaxDb; O14529; -.
DR PRIDE; O14529; -.
DR DNASU; 23316; -.
DR Ensembl; ENST00000261726; ENSP00000261726; ENSG00000111249.
DR GeneID; 23316; -.
DR KEGG; hsa:23316; -.
DR UCSC; uc001tsa.2; human.
DR CTD; 23316; -.
DR GeneCards; GC12P111471; -.
DR HGNC; HGNC:19347; CUX2.
DR HPA; HPA044772; -.
DR MIM; 610648; gene.
DR neXtProt; NX_O14529; -.
DR PharmGKB; PA162382977; -.
DR eggNOG; NOG325167; -.
DR HOGENOM; HOG000143386; -.
DR HOVERGEN; HBG051268; -.
DR InParanoid; O14529; -.
DR KO; K09313; -.
DR OMA; LVFPPAF; -.
DR OrthoDB; EOG4K9BBG; -.
DR EvolutionaryTrace; O14529; -.
DR GenomeRNAi; 23316; -.
DR NextBio; 45200; -.
DR ArrayExpress; O14529; -.
DR Bgee; O14529; -.
DR CleanEx; HS_CUX2; -.
DR Genevestigator; O14529; -.
DR GermOnline; ENSG00000111249; Homo sapiens.
DR GO; GO:0005634; C:nucleus; NAS:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0003700; F:sequence-specific DNA binding transcription factor activity; NAS:UniProtKB.
DR GO; GO:0071310; P:cellular response to organic substance; IEA:Compara.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
DR GO; GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.60; -; 1.
DR Gene3D; 1.10.260.40; -; 3.
DR InterPro; IPR003350; Hmoeo_CUT.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeodomain.
DR InterPro; IPR009057; Homeodomain-like.
DR InterPro; IPR010982; Lambda_DNA-bd_dom.
DR Pfam; PF02376; CUT; 3.
DR Pfam; PF00046; Homeobox; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; Homeodomain_like; 1.
DR SUPFAM; SSF47413; Lambda_like_DNA; 3.
DR PROSITE; PS51042; CUT; 3.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; Complete proteome; DNA-binding; Homeobox;
KW Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat;
KW Transcription; Transcription regulation.
FT CHAIN 1 1486 Homeobox protein cut-like 2.
FT /FTId=PRO_0000202396.
FT DNA_BIND 544 631 CUT 1.
FT DNA_BIND 887 974 CUT 2.
FT DNA_BIND 1038 1125 CUT 3.
FT DNA_BIND 1168 1227 Homeobox.
FT COILED 195 374 Potential.
FT COILED 690 717 Potential.
FT COMPBIAS 440 535 Pro-rich.
FT COMPBIAS 802 813 Poly-Ser.
FT COMPBIAS 1327 1437 Pro-rich.
FT MOD_RES 143 143 Phosphoserine.
FT VARIANT 1472 1472 V -> L (in dbSNP:rs6490073).
FT /FTId=VAR_065096.
FT HELIX 555 568
FT HELIX 573 579
FT HELIX 585 593
FT HELIX 598 600
FT HELIX 603 616
FT HELIX 620 630
FT HELIX 889 893
FT HELIX 898 910
FT TURN 911 913
FT HELIX 916 922
FT HELIX 928 936
FT TURN 941 943
FT HELIX 946 961
FT HELIX 1037 1043
FT HELIX 1049 1062
FT HELIX 1067 1073
FT HELIX 1079 1087
FT TURN 1092 1094
FT HELIX 1097 1111
FT HELIX 1115 1123
SQ SEQUENCE 1486 AA; 161677 MW; 71782EF5214D0262 CRC64;
MAANVGSMFQ YWKRFDLRRL QKELNSVASE LSARQEESEH SHKHLIELRR EFKKNVPEEI
REMVAPVLKS FQAEVVALSK RSQEAEAAFL SVYKQLIEAP DPVPVFEAAR SLDDRLQPPS
FDPSGQPRRD LHTSWKRNPE LLSPKEQREG TSPAGPTLTE GSRLPGIPGK ALLTETLLQR
NEAEKQKGLQ EVQITLAARL GEAEEKIKVL HSALKATQAE LLELRRKYDE EAASKADEVG
LIMTNLEKAN QRAEAAQREV ESLREQLASV NSSIRLACCS PQGPSGDKVN FTLCSGPRLE
AALASKDREI LRLLKDVQHL QSSLQELEEA SANQIADLER QLTAKSEAIE KLEEKLQAQS
DYEEIKTELS ILKAMKLASS TCSLPQGMAK PEDSLLIAKE AFFPTQKFLL EKPSLLASPE
EDPSEDDSIK DSLGTEQSYP SPQQLPPPPG PEDPLSPSPG QPLLGPSLGP DGTRTFSLSP
FPSLASGERL MMPPAAFKGE AGGLLVFPPA FYGAKPPTAP ATPAPGPEPL GGPEPADGGG
GGAAGPGAEE EQLDTAEIAF QVKEQLLKHN IGQRVFGHYV LGLSQGSVSE ILARPKPWRK
LTVKGKEPFI KMKQFLSDEQ NVLALRTIQV RQRGSITPRI RTPETGSDDA IKSILEQAKK
EIESQKGGEP KTSVAPLSIA NGTTPASTSE DAIKSILEQA RREMQAQQQA LLEMEVAPRG
RSVPPSPPER PSLATASQNG APALVKQEEG SGGPAQAPLP VLSPAAFVQS IIRKVKSEIG
DAGYFDHHWA SDRGLLSRPY ASVSPSLSSS SSSGYSGQPN GRAWPRGDEA PVPPEDEAAA
GAEDEPPRTG ELKAEGATAE AGARLPYYPA YVPRTLKPTV PPLTPEQYEL YMYREVDTLE
LTRQVKEKLA KNGICQRIFG EKVLGLSQGS VSDMLSRPKP WSKLTQKGRE PFIRMQLWLS
DQLGQAVGQQ PGASQASPTE PRSSPSPPPS PTEPEKSSQE PLSLSLESSK ENQQPEGRSS
SSLSGKMYSG SQAPGGIQEI VAMSPELDTY SITKRVKEVL TDNNLGQRLF GESILGLTQG
SVSDLLSRPK PWHKLSLKGR EPFVRMQLWL NDPHNVEKLR DMKKLEKKAY LKRRYGLIST
GSDSESPATR SECPSPCLQP QDLSLLQIKK PRVVLAPEEK EALRKAYQLE PYPSQQTIEL
LSFQLNLKTN TVINWFHNYR SRMRREMLVE GTQDEPDLDP SGGPGILPPG HSHPDPTPQS
PDSETEDQKP TVKELELQEG PEENSTPLTT QDKAQVRIKQ EQMEEDAEEE AGSQPQDSGE
LDKGQGPPKE EHPDPPGNDG LPKVAPGPLL PGGSTPDCPS LHPQQESEAG ERLHPDPLSF
KSASESSRCS LEVSLNSPSA ASSPGLMMSV SPVPSSSAPI SPSPPGAPPA KVPSASPTAD
MAGALHPSAK VNPNLQRRHE KMANLNNIIY RVERAANREE ALEWEF
//
