ID EFTU_RECAM Reviewed; 394 AA.
AC O21245;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 13-SEP-2023, entry version 96.
DE RecName: Full=Elongation factor Tu, mitochondrial;
GN Name=TUFA;
OS Reclinomonas americana.
OG Mitochondrion.
OC Eukaryota; Discoba; Jakobida; Histionina; Histionidae; Reclinomonas.
OX NCBI_TaxID=48483;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 50394;
RX PubMed=9168110; DOI=10.1038/387493a0;
RA Lang B.F., Burger G., O'Kelly C.J., Cedergren R., Golding G.B., Lemieux C.,
RA Sankoff D., Turmel M., Gray M.W.;
RT "An ancestral mitochondrial DNA resembling a eubacterial genome in
RT miniature.";
RL Nature 387:493-497(1997).
CC -!- FUNCTION: This protein promotes the GTP-dependent binding of aminoacyl-
CC tRNA to the A-site of ribosomes during protein biosynthesis.
CC -!- SUBCELLULAR LOCATION: Mitochondrion.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A
CC subfamily. {ECO:0000305}.
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DR EMBL; AF007261; AAD11872.1; -; Genomic_DNA.
DR PIR; S78139; S78139.
DR RefSeq; NP_044757.1; NC_001823.1.
DR AlphaFoldDB; O21245; -.
DR SMR; O21245; -.
DR GeneID; 801090; -.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR CDD; cd01884; EF_Tu; 1.
DR CDD; cd03697; EFTU_II; 1.
DR CDD; cd03707; EFTU_III; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 2.
DR HAMAP; MF_00118_B; EF_Tu_B; 1.
DR InterPro; IPR041709; EF-Tu_GTP-bd.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR033720; EFTU_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004541; Transl_elong_EFTu/EF1A_bac/org.
DR InterPro; IPR004160; Transl_elong_EFTu/EF1A_C.
DR NCBIfam; TIGR00485; EF-Tu; 1.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR PANTHER; PTHR43721:SF22; ELONGATION FACTOR TU, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43721; ELONGATION FACTOR TU-RELATED; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF03143; GTP_EFTU_D3; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50465; EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 3: Inferred from homology;
KW Elongation factor; GTP-binding; Mitochondrion; Nucleotide-binding;
KW Protein biosynthesis.
FT CHAIN 1..394
FT /note="Elongation factor Tu, mitochondrial"
FT /id="PRO_0000091472"
FT DOMAIN 10..204
FT /note="tr-type G"
FT REGION 19..26
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 60..64
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 81..84
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 136..139
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 174..176
FT /note="G5"
FT /evidence="ECO:0000250"
FT BINDING 19..26
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 81..85
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 136..139
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
SQ SEQUENCE 394 AA; 43627 MW; 428202ADF3DBC4EF CRC64;
MSKEKFERTK PHCNIGTIGH VDHGKTTLTA AITKVLSETG GAVFTDYDQI DKAPEEKKRG
ITISTSHVEY ETTKRHYAHI DCPGHEDYVK NMITGAAQMD GAILVVSAVD GPMPQTREHI
LLSRQVGVPS LVVFLNKVDM VNDPEMLDLV EMEVRELLLS YKYPGDEIPI IRGSALKALQ
GEIEYKKSIL KLMEAVDNYI PQPERSFDRP FLMPVEDVFS IAGRGTVVTG RVEQGQIKIG
DAVEIIGLGS TVKTTCTGIE MFHKLLDYGQ AGDNLGMLIR GIQRDAVQRG QVICAPGSVK
PHTKYEAQVY ILTKEEGGRH KPFFNNYRPQ FFFRTADVTG TIQLPKDVEM VNPGDNVKLI
IELITPIAME EGIRFAMREG GRTIGAGVVS KIIE
//
