ID O24625_NICSY Unreviewed; 363 AA.
AC O24625;
DT 01-JAN-1998, integrated into UniProtKB/TrEMBL.
DT 01-JAN-1998, sequence version 1.
DT 27-MAR-2024, entry version 93.
DE RecName: Full=chalcone synthase {ECO:0000256|ARBA:ARBA00012975};
DE EC=2.3.1.74 {ECO:0000256|ARBA:ARBA00012975};
GN Name=NSCHSLK {ECO:0000313|EMBL:CAA74847.1};
OS Nicotiana sylvestris (Wood tobacco) (South American tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4096 {ECO:0000313|EMBL:CAA74847.1};
RN [1] {ECO:0000313|EMBL:CAA74847.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Anther {ECO:0000313|EMBL:CAA74847.1};
RX PubMed=9869422; DOI=10.1023/A:1006074508779;
RA Atanassov I., Russinova E., Antonov L., Atanassov A.;
RT "Expression of an anther-specific chalcone synthase-like gene is correlated
RT with uninucleate microspore development in Nicotiana sylvestris.";
RL Plant Mol. Biol. 38:1169-1178(1998).
CC -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC which can under specific conditions spontaneously isomerize into
CC naringenin. {ECO:0000256|ARBA:ARBA00002969}.
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004966}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000256|ARBA:ARBA00005531,
CC ECO:0000256|RuleBase:RU003633}.
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DR EMBL; Y14506; CAA74846.1; -; mRNA.
DR EMBL; Y14507; CAA74847.1; -; mRNA.
DR PIR; T15054; T15054.
DR AlphaFoldDB; O24625; -.
DR SMR; O24625; -.
DR UniPathway; UPA00154; -.
DR GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR PANTHER; PTHR11877:SF46; TYPE III POLYKETIDE SYNTHASE A; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 2: Evidence at transcript level;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000256|RuleBase:RU003633};
KW Flavonoid biosynthesis {ECO:0000256|ARBA:ARBA00023241};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU003633}.
FT DOMAIN 1..206
FT /note="Chalcone/stilbene synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00195"
FT DOMAIN 216..363
FT /note="Chalcone/stilbene synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02797"
FT ACT_SITE 142
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000451-1"
SQ SEQUENCE 363 AA; 40284 MW; C4AEE6EA4FECE5A1 CRC64;
MGKAFPAQLV PQDCLVEGYI RDTNCQDLAI KEKLERLCKT TTVKTRYTVM SKEILDKYPE
LATEGTPTIK QRLEIANPAV VEMAKQASQA CIKEWGRSAE EITHIVYVSS SEIRLPGGDL
YLATELGLRN DIGRVMLYFL GCYGGVTGLR VAKDIAENNP GSRVLLTTSE TTILGFRPPN
KARPYDLVGA ALFGDGAAAV IIGTEPIMGK ESPFMELNFA TQQFLPGTNN VIDGRLTEEG
INFKLGRDLP EKIQDNIEEF CKKIIAKADL REAKYNDLFW AVHPGGPAIL NRLENTLKLQ
SEKLDCSRRA LMDYGNVSSN TIFYVMEYMR EELKNKKNGG EEWGLALAFG PGITFEGILL
RSL
//
