GenomeNet

Database: UniProt
Entry: O27720
LinkDB: O27720
Original site: O27720 
ID   THII_METTH              Reviewed;         389 AA.
AC   O27720;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-SEP-2014, entry version 91.
DE   RecName: Full=Probable tRNA sulfurtransferase;
DE            EC=2.8.1.4;
DE   AltName: Full=Sulfur carrier protein ThiS sulfurtransferase;
DE   AltName: Full=Thiamine biosynthesis protein ThiI;
DE   AltName: Full=tRNA 4-thiouridine synthase;
GN   Name=thiI; OrderedLocusNames=MTH_1685;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 /
OS   JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium
OS   thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales;
OC   Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K.,
RA   Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D.,
RA   Spadafora R., Vicare R., Wang Y., Wierzbowski J., Gibson R.,
RA   Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S.,
RA   McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M.,
RA   Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum
RT   deltaH: functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- FUNCTION: Catalyzes the ATP-dependent transfer of a sulfur to tRNA
CC       to produce 4-thiouridine in position 8 of tRNAs, which functions
CC       as a near-UV photosensor. Also catalyzes the transfer of sulfur to
CC       the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate.
CC       This is a step in the synthesis of thiazole, in the thiamine
CC       biosynthesis pathway. The sulfur is donated as persulfide by IscS
CC       (By similarity).
CC   -!- CATALYTIC ACTIVITY: L-cysteine + 'activated' tRNA = L-serine +
CC       tRNA containing a thionucleotide.
CC   -!- CATALYTIC ACTIVITY: [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH +
CC       [ThiS]-COSH + AMP.
CC   -!- PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the ThiI family.
CC   -!- SIMILARITY: Contains 1 THUMP domain.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AE000666; AAB86157.1; -; Genomic_DNA.
DR   PIR; C69092; C69092.
DR   RefSeq; NP_276796.1; NC_000916.1.
DR   RefSeq; WP_010877292.1; NC_000916.1.
DR   ProteinModelPortal; O27720; -.
DR   STRING; 187420.MTH1685; -.
DR   EnsemblBacteria; AAB86157; AAB86157; MTH_1685.
DR   GeneID; 1470770; -.
DR   KEGG; mth:MTH1685; -.
DR   eggNOG; COG0301; -.
DR   KO; K03151; -.
DR   OMA; AQMKGPD; -.
DR   BioCyc; MTHE187420:GJNM-1687-MONOMER; -.
DR   UniPathway; UPA00060; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0016783; F:sulfurtransferase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0009228; P:thiamine biosynthetic process; IEA:UniProtKB-HAMAP.
DR   GO; GO:0009229; P:thiamine diphosphate biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0034227; P:tRNA thio-modification; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_00021; ThiI; 1.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR020536; ThiI_AANH.
DR   InterPro; IPR004114; THUMP.
DR   InterPro; IPR003720; tRNA_STrfase.
DR   Pfam; PF02568; ThiI; 1.
DR   Pfam; PF02926; THUMP; 1.
DR   SMART; SM00981; THUMP; 1.
DR   TIGRFAMs; TIGR00342; TIGR00342; 1.
DR   PROSITE; PS51165; THUMP; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; Nucleotide-binding;
KW   Reference proteome; RNA-binding; Thiamine biosynthesis; Transferase;
KW   tRNA-binding.
FT   CHAIN         1    389       Probable tRNA sulfurtransferase.
FT                                /FTId=PRO_0000154896.
FT   DOMAIN       57    165       THUMP.
FT   NP_BIND     183    184       ATP (By similarity).
FT   BINDING     267    267       ATP (By similarity).
FT   BINDING     289    289       ATP; via amide nitrogen (By similarity).
FT   BINDING     298    298       ATP (By similarity).
SQ   SEQUENCE   389 AA;  42473 MW;  CBB6B788018AACE6 CRC64;
     MILDYDVILA RYGEVAIKGP SVRRRFEGKL LHNIKSAFSC RAELRHGRIF IFPEDMDEAL
     DRLSKIFGIV SFSPAVTAET GFDSIEDSLR EYIHELRSEG LLTSRTPFAI RCRRVGEHDF
     TSQEMAAFAG SVVVGEIGAP VDLGNPDLEI HLEIREDETY IYHRVIPGPG GLPAGTQGKV
     VALLSGGIDS PVATYLMMKR GCQVVAVHMD NAPFTGEEAE EKVEKIAAKL AEYSAGVEFK
     LRTFSYGRYL ESCRRGAPEK MTCVLCKFGM YHLAEMVAEE EGALAIVDGS SLGQVASQTL
     PNILATRMGV NIPILSPLIG MDKVEIENLA KRIGTYDISV IPDGGCSAVP AHPSTASPPE
     AVMEASEKIN VKEEVAEIFR KGSKTRIFS
//
DBGET integrated database retrieval system