ID O32728_GEOSE Unreviewed; 250 AA.
AC O32728;
DT 01-JAN-1998, integrated into UniProtKB/TrEMBL.
DT 01-JAN-1998, sequence version 1.
DT 13-SEP-2023, entry version 129.
DE RecName: Full=RNA polymerase sigma factor {ECO:0000256|RuleBase:RU362124};
OS Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX NCBI_TaxID=1422 {ECO:0000313|EMBL:AAB81194.1};
RN [1] {ECO:0000313|EMBL:AAB81194.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=9266669; DOI=10.1016/S0378-1119(97)00096-6;
RA Park S.G., Yudkin M.D.;
RT "Sequencing and phylogenetic analysis of the spoIIA operon from diverse
RT Bacillus and Paenibacillus species.";
RL Gene 194:25-33(1997).
RN [2] {ECO:0007829|PDB:1L0O}
RP X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 7-245.
RX PubMed=11955433; DOI=10.1016/S0092-8674(02)00662-1;
RA Campbell E.A., Masuda S., Sun J.L., Muzzin O., Olson C.A., Wang S.,
RA Darst S.A.;
RT "Crystal structure of the Bacillus stearothermophilus anti-sigma factor
RT SpoIIAB with the sporulation sigma factor sigmaF.";
RL Cell 108:795-807(2002).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. {ECO:0000256|RuleBase:RU362124}.
CC -!- INTERACTION:
CC O32728; O32727: spoIIAB; NbExp=5; IntAct=EBI-1033248, EBI-1033242;
CC -!- SIMILARITY: Belongs to the sigma-70 factor family.
CC {ECO:0000256|ARBA:ARBA00007788, ECO:0000256|RuleBase:RU362124}.
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DR EMBL; L47360; AAB81194.1; -; Genomic_DNA.
DR PDB; 1L0O; X-ray; 2.90 A; C=7-245.
DR PDBsum; 1L0O; -.
DR AlphaFoldDB; O32728; -.
DR SMR; O32728; -.
DR IntAct; O32728; 1.
DR EvolutionaryTrace; O32728; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription initiation; IEA:InterPro.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR CDD; cd06171; Sigma70_r4; 1.
DR DisProt; DP00902; -.
DR Gene3D; 1.20.120.1810; -; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 2.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR014322; RNA_pol_sigma-B/F/G.
DR InterPro; IPR014236; RNA_pol_sigma-F.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR NCBIfam; TIGR02980; SigBFG; 1.
DR NCBIfam; TIGR02937; sigma70-ECF; 1.
DR NCBIfam; TIGR02885; spore_sigF; 1.
DR PANTHER; PTHR30603; RNA POLYMERASE SIGMA FACTOR RPO; 1.
DR PANTHER; PTHR30603:SF19; RNA POLYMERASE SIGMA-F FACTOR; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88946; Sigma2 domain of RNA polymerase sigma factors; 1.
DR SUPFAM; SSF88659; Sigma3 and sigma4 domains of RNA polymerase sigma factors; 2.
DR PROSITE; PS50943; HTH_CROC1; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:1L0O};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU362124};
KW Sigma factor {ECO:0000256|ARBA:ARBA00023082,
KW ECO:0000256|RuleBase:RU362124};
KW Sporulation {ECO:0000256|ARBA:ARBA00022969};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU362124};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015,
KW ECO:0000256|RuleBase:RU362124}.
FT DOMAIN 208..238
FT /note="HTH cro/C1-type"
FT /evidence="ECO:0000259|PROSITE:PS50943"
SQ SEQUENCE 250 AA; 28775 MW; D0B9CBC7DDE41465 CRC64;
MDVDVKQGQS PIKDQEMKEL IRRSQEGDQE ARDEIIEKNM RLVWSVVQRF LNRGYEADDL
FQIGCIGLLK SVDKFDLSYD VKFSTYAVPM IIGEIQRFLR DDGTVKVSRS LKEMGNKIRK
AKDELSKTRG RSPTITEIAD HLGISPEDVV LAQEAVRLPT SIHETVYEND GDPITLLDQI
ADADEASWFD KIALKKAIEE LDERERLIVY LRYYRDRTQS EVASRLGISQ VQVSRLEKKI
LQHIKDKMDG
//
