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Database: UniProt
Entry: O34748
LinkDB: O34748
Original site: O34748 
ID   RECQ_BACSU              Reviewed;         591 AA.
AC   O34748; Q796C5;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   16-APR-2014, entry version 100.
DE   RecName: Full=Probable ATP-dependent DNA helicase RecQ;
DE            EC=3.6.4.12;
GN   Name=recQ; Synonyms=yocI; OrderedLocusNames=BSU19220;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT   "Sequence analysis of the Bacillus subtilis chromosome region between
RT   the terC and odhAB loci cloned in a yeast artificial chromosome.";
RL   Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   IDENTIFICATION.
RC   STRAIN=168 / YB886 / BG214;
RX   PubMed=9642195;
RA   Fernandez S., Sorokin A., Alonso J.C.;
RT   "Genetic recombination in Bacillus subtilis 168: effects of recU and
RT   recS mutations on DNA repair and homologous recombination.";
RL   J. Bacteriol. 180:3405-3409(1998).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=168 / YB886 / BG214;
RX   PubMed=16385024; DOI=10.1128/JB.188.2.353-360.2006;
RA   Sanchez H., Kidane D., Castillo Cozar M., Graumann P.L., Alonso J.C.;
RT   "Recruitment of Bacillus subtilis RecN to DNA double-strand breaks in
RT   the absence of DNA end processing.";
RL   J. Bacteriol. 188:353-360(2006).
CC   -!- FUNCTION: Probable DNA helicase. Required for DNA repair and
CC       intramolecular recombination; probably has overlapping function
CC       with RecS (AC P50729). It probably acts to help generate ss-DNA
CC       from ds-DNA breaks.
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid. Note=Localized
CC       throughout the nucleoid in the presence or absence of DNA double-
CC       strand breaks.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene are moderately
CC       sensitive to DNA-damaging agents. Sensitivity increases in the
CC       presence of mutated AddAB nuclease.
CC   -!- SIMILARITY: Belongs to the helicase family. RecQ subfamily.
CC   -!- SIMILARITY: Contains 1 helicase ATP-binding domain.
CC   -!- SIMILARITY: Contains 1 helicase C-terminal domain.
CC   -!- SIMILARITY: Contains 1 HRDC domain.
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DR   EMBL; AF027868; AAB84475.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB13814.1; -; Genomic_DNA.
DR   PIR; F69901; F69901.
DR   RefSeq; NP_389803.1; NC_000964.3.
DR   ProteinModelPortal; O34748; -.
DR   SMR; O34748; 1-506, 515-590.
DR   STRING; 224308.BSU19220; -.
DR   PaxDb; O34748; -.
DR   EnsemblBacteria; CAB13814; CAB13814; BSU19220.
DR   GeneID; 939671; -.
DR   KEGG; bsu:BSU19220; -.
DR   PATRIC; 18975693; VBIBacSub10457_2037.
DR   GenoList; BSU19220; -.
DR   eggNOG; COG0514; -.
DR   HOGENOM; HOG000044388; -.
DR   KO; K03654; -.
DR   OMA; KENTVYT; -.
DR   OrthoDB; EOG6ZSPDC; -.
DR   ProtClustDB; CLSK887416; -.
DR   BioCyc; BSUB:BSU19220-MONOMER; -.
DR   GO; GO:0043590; C:bacterial nucleoid; IDA:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043140; F:ATP-dependent 3'-5' DNA helicase activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 1.10.150.80; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
DR   InterPro; IPR004589; DNA_helicase_ATP-dep_RecQ.
DR   InterPro; IPR006293; DNA_helicase_ATP-dep_RecQ_bac.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR010997; HRDC-like.
DR   InterPro; IPR002121; HRDC_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR018982; RQC_domain.
DR   InterPro; IPR011991; WHTH_DNA-bd_dom.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF00570; HRDC; 1.
DR   Pfam; PF09382; RQC; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SMART; SM00341; HRDC; 1.
DR   SMART; SM00956; RQC; 1.
DR   SUPFAM; SSF47819; SSF47819; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01389; recQ; 1.
DR   TIGRFAMs; TIGR00614; recQ_fam; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS50967; HRDC; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; DNA damage;
KW   DNA recombination; DNA repair; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome; SOS response.
FT   CHAIN         1    591       Probable ATP-dependent DNA helicase RecQ.
FT                                /FTId=PRO_0000379511.
FT   DOMAIN       27    196       Helicase ATP-binding.
FT   DOMAIN      216    367       Helicase C-terminal.
FT   DOMAIN      513    591       HRDC.
FT   NP_BIND      40     47       ATP (By similarity).
FT   MOTIF       139    142       DEAH box.
SQ   SEQUENCE   591 AA;  67353 MW;  D66395DD59153626 CRC64;
     MLHRAQSLLA HYFGYEKFRS GQDEAIRLVT EARQNTACIM PTGGGKSICY QIPALMFEGT
     TIVISPLISL MKDQVDALEE AGINAAYINS TQSNQEIYER LNGLKEGAYK LFYITPERLT
     SIEFIRILQG IDVPLVAIDE AHCISQWGHD FRPSYRNIEI LFRELHDKPV IMALTATATP
     EVHDDICKQL HIQKENTVYT GFSRENLTFK VVKGENKDRF IDEYVQNNRH EAGIVYTATR
     KEADRIYERL KRNQVRAGRY HGGLADDVRK EQQERFLNDE LQVMVATSAF GMGIDKSNIR
     FVLHAQIPKD MESYYQEAGR AGRDGLASEC VLLFSPQDIM VQRFLIEQSE HEEKQKQDLK
     KLRQMVDYCH TEDCLQRFIL MYFGEKEPDA CGQCGNCTDT RAAHDVTREA QMVLSCIIRM
     KERFGKTMVA QVLAGSKNKK VLENGFSDLS TYGILKHQSV GEISDFIEFL ISDDFIRMSD
     GTFPTLFVSS KGRNVLKGEL SVARKEALKA AAITENDELF ERLRMVRKEI AAEQGVPPFV
     VFSDQTLKEM SGKQPVNDDE LLSIKGVGEQ KRAKYGRLFL QEIQAYARMT D
//
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