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Database: UniProt
Entry: O46037
LinkDB: O46037
Original site: O46037 
ID   VINC_DROME              Reviewed;         961 AA.
AC   O46037;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   01-OCT-2014, entry version 101.
DE   RecName: Full=Vinculin;
GN   Name=Vinc; ORFNames=CG3299;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
OC   Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha;
OC   Ephydroidea; Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9280281; DOI=10.1016/S0014-5793(97)00901-0;
RA   Alatortsev V.E., Kramerova I.A., Frolov M.V., Lavrov S.A.,
RA   Westphal E.D.;
RT   "Vinculin gene is non-essential in Drosophila melanogaster.";
RL   FEBS Lett. 413:197-201(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X.,
RA   Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D.,
RA   Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G.,
RA   Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D.,
RA   Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M.,
RA   Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S.,
RA   Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P.,
RA   Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I.,
RA   Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P.,
RA   de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M.,
RA   Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P.,
RA   Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W.,
RA   Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K.,
RA   Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J.,
RA   Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C.,
RA   Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A.,
RA   Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z.,
RA   Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X.,
RA   Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D.,
RA   Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A.,
RA   Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L.,
RA   Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M.,
RA   Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G.,
RA   Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H.,
RA   Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E.,
RA   Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X.,
RA   Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J.,
RA   Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A.,
RA   Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L.,
RA   Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X.,
RA   Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q.,
RA   Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M.,
RA   Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a
RT   systematic review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Oregon-R;
RX   PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA   Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D.,
RA   Barrell B.G., Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E.,
RA   Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Borkova D.,
RA   Minana B., Kafatos F.C., Louis C., Siden-Kiamos I., Bolshakov S.,
RA   Papagiannakis G., Spanos L., Cox S., Madueno E., de Pablos B.,
RA   Modolell J., Peter A., Schoettler P., Werner M., Mourkioti F.,
RA   Beinert N., Dowe G., Schaefer U., Jaeckle H., Bucheton A.,
RA   Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA   McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA   Glover D.M.;
RT   "From sequence to chromosome: the tip of the X chromosome of D.
RT   melanogaster.";
RL   Science 287:2220-2222(2000).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Embryo;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M.,
RA   George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H.,
RA   Rubin G.M., Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-774, AND IDENTIFICATION
RP   BY MASS SPECTROMETRY.
RX   PubMed=17372656; DOI=10.1039/b617545g;
RA   Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D.,
RA   Juenger M.A., Eng J.K., Aebersold R., Tao W.A.;
RT   "An integrated chemical, mass spectrometric and computational strategy
RT   for (quantitative) phosphoproteomics: application to Drosophila
RT   melanogaster Kc167 cells.";
RL   Mol. Biosyst. 3:275-286(2007).
CC   -!- FUNCTION: Involved in cell adhesion. May be involved in the
CC       attachment of the actin-based microfilaments to the plasma
CC       membrane (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Exhibits self-association properties. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}. Cell
CC       junction, adherens junction {ECO:0000250}. Cell membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250};
CC       Cytoplasmic side {ECO:0000250}. Cell junction {ECO:0000250}.
CC       Note=Cytoplasmic face of adhesion plaques. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the vinculin/alpha-catenin family.
CC       {ECO:0000305}.
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DR   EMBL; AE014298; AAF45752.1; -; Genomic_DNA.
DR   EMBL; AL009193; CAA15691.1; -; Genomic_DNA.
DR   EMBL; AY128501; AAM75094.1; -; mRNA.
DR   RefSeq; NP_476820.1; NM_057472.2.
DR   UniGene; Dm.32; -.
DR   ProteinModelPortal; O46037; -.
DR   SMR; O46037; 1-955.
DR   BioGrid; 57740; 10.
DR   IntAct; O46037; 2.
DR   MINT; MINT-742946; -.
DR   STRING; 7227.FBpp0070404; -.
DR   PaxDb; O46037; -.
DR   PRIDE; O46037; -.
DR   EnsemblMetazoa; FBtr0070420; FBpp0070404; FBgn0004397.
DR   GeneID; 31201; -.
DR   KEGG; dme:Dmel_CG3299; -.
DR   CTD; 31201; -.
DR   FlyBase; FBgn0004397; Vinc.
DR   eggNOG; NOG329927; -.
DR   GeneTree; ENSGT00550000074411; -.
DR   InParanoid; O46037; -.
DR   KO; K05700; -.
DR   OMA; LTHQVHR; -.
DR   OrthoDB; EOG73NG2V; -.
DR   PhylomeDB; O46037; -.
DR   GenomeRNAi; 31201; -.
DR   NextBio; 772430; -.
DR   PRO; PR:O46037; -.
DR   Bgee; O46037; -.
DR   GO; GO:0015629; C:actin cytoskeleton; IEA:InterPro.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005925; C:focal adhesion; ISS:FlyBase.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003779; F:actin binding; ISS:FlyBase.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR   GO; GO:0007016; P:cytoskeletal anchoring at plasma membrane; ISS:FlyBase.
DR   GO; GO:0048812; P:neuron projection morphogenesis; IMP:FlyBase.
DR   GO; GO:0006909; P:phagocytosis; IMP:FlyBase.
DR   InterPro; IPR017997; Vinculin.
DR   InterPro; IPR006077; Vinculin/catenin.
DR   InterPro; IPR000633; Vinculin_CS.
DR   PANTHER; PTHR18914; PTHR18914; 1.
DR   Pfam; PF01044; Vinculin; 1.
DR   PRINTS; PR00806; VINCULIN.
DR   SUPFAM; SSF47220; SSF47220; 7.
DR   PROSITE; PS00664; VINCULIN_2; 1.
PE   1: Evidence at protein level;
KW   Actin-binding; Cell adhesion; Cell junction; Cell membrane;
KW   Complete proteome; Cytoplasm; Cytoskeleton; Membrane; Phosphoprotein;
KW   Reference proteome; Repeat.
FT   CHAIN         1    961       Vinculin.
FT                                /FTId=PRO_0000064259.
FT   REPEAT      258    362       1.
FT   REPEAT      371    470       2.
FT   REGION      258    470       2 X repeats.
FT   COMPBIAS    723    772       Pro-rich.
FT   MOD_RES     774    774       Phosphothreonine.
FT                                {ECO:0000269|PubMed:17372656}.
SQ   SEQUENCE   961 AA;  106302 MW;  17E50E11F507C875 CRC64;
     MPVFHTKTIE SILDPVAQQV SRLVILHEEA EDGNAMPDLS RPVQVVSAAV ANLVKVGRDT
     INSSDDKILR QDMPSALHRV EGASQLLEEA SDMLRSDPYS GPARKKLIEG SRGILQGTSS
     LLLCFDESEV RKIIQECKRV LDYLAVAEVI NTMEQLVQFL KDLSPCLSKV HREVGAREKE
     LTHQVHSEIL VRCLEQVKTL APILICSMKV YIHIVEQQGR GAEEAAENRN YLAARMSDEL
     QEIIRVLQLT TYDEDTSELD NLTVLKKLSN AISNKMEQAN EWLSNPYALR GGVGEKALRQ
     VIDNATEISE RCLPQDSYPI RKLADEVTAM ANTLCELRQE GKGQSPQAES LVRGIRDRMG
     ELKSLVHQAV LGVDKAGVQQ TAHTIQGRLE QAVKWLQHPE INDGGLGERA INLIVEEGRK
     VAEGCPGHQK AEIQQLCDEV ERLKRQAAGS GPAAKQAAKQ LTQKLYELKA AIQNALVNRI
     VQDFMDVSTP LKQFTEAVLQ PEGTPGREQN FNQKSNNLQA FSDRASKTSR MVAAGGACGN
     KKIAEILLSS AAQVDSLTPQ LISAGRIRMN YPGSKAADEH LQNLKQQYAD TVLRMRTLCD
     QATDPADFIK TSEEHMQVYA KLCEDAIHAR QPQKMVDNTS NIARLINRVL LVAKQEADNS
     EDPVFTERLN AAANRLERSL PAMVGDAKLV ATNIADPAAA AAWKNSFQRL LGDVREVRDA
     IAPPQPPPLP TSLPPPIPEL SALHLSNQNA ERAPPRPPLP REGLAPVRPP PPETDDEDEG
     VFRTMPHANQ PILIAARGLH QEVRQWSSKD NEIIAAAKRM AILMARLSEL VLSDSRGSKR
     ELIATAKKIA EASEDVTRLA KELARQCTDR RIRTNLLQVC ERIPTIGTQL KILSTVKATM
     LGAQGSDEDR EATEMLVGNA QNLMQSVKET VRAAEGASIK IRSDQTSNRL QWVRRQPWYQ
     Y
//
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