ID GLMS_PYRHO Reviewed; 598 AA.
AC O57981;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 01-MAY-2013, entry version 89.
DE RecName: Full=Glutamine--fructose-6-phosphate aminotransferase [isomerizing];
DE EC=2.6.1.16;
DE AltName: Full=D-fructose-6-phosphate amidotransferase;
DE AltName: Full=GFAT;
DE AltName: Full=Glucosamine-6-phosphate synthase;
DE AltName: Full=Hexosephosphate aminotransferase;
DE AltName: Full=L-glutamine--D-fructose-6-phosphate amidotransferase;
GN Name=glmS; OrderedLocusNames=PH0243;
OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 /
OS NBRC 100139 / OT-3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=70601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y.,
RA Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y.,
RA Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y.,
RA Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A.,
RA Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K.,
RA Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
CC -!- FUNCTION: Catalyzes the first step in hexosamine metabolism,
CC converting fructose-6P into glucosamine-6P using glutamine as a
CC nitrogen source (By similarity).
CC -!- CATALYTIC ACTIVITY: L-glutamine + D-fructose 6-phosphate = L-
CC glutamate + D-glucosamine 6-phosphate.
CC -!- SUBUNIT: Homodimer (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- SIMILARITY: Contains 1 glutamine amidotransferase type-2 domain.
CC -!- SIMILARITY: Contains 2 SIS domains.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA29315.1; Type=Erroneous initiation;
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DR EMBL; BA000001; BAA29315.1; ALT_INIT; Genomic_DNA.
DR PIR; D71248; D71248.
DR RefSeq; NP_142239.1; NC_000961.1.
DR ProteinModelPortal; O57981; -.
DR STRING; 70601.PH0243; -.
DR PRIDE; O57981; -.
DR EnsemblBacteria; BAA29315; BAA29315; BAA29315.
DR GeneID; 1444133; -.
DR KEGG; pho:PH0243; -.
DR eggNOG; COG0449; -.
DR HOGENOM; HOG000258898; -.
DR KO; K00820; -.
DR OMA; CLQGLER; -.
DR ProtClustDB; PRK00331; -.
DR BioCyc; PHOR70601:GJWR-234-MONOMER; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0004360; F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity; IEA:HAMAP.
DR GO; GO:0016051; P:carbohydrate biosynthetic process; IEA:InterPro.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:HAMAP.
DR HAMAP; MF_00164; GlmS; 1; -.
DR InterPro; IPR017932; GATase_2_dom.
DR InterPro; IPR000583; GATase_dom.
DR InterPro; IPR005855; GlmS_trans.
DR InterPro; IPR001347; SIS.
DR PANTHER; PTHR10937:SF0; PTHR10937:SF0; 1.
DR Pfam; PF00310; GATase_2; 1.
DR Pfam; PF01380; SIS; 2.
DR TIGRFAMs; TIGR01135; glmS; 1.
DR PROSITE; PS51278; GATASE_TYPE_2; 1.
DR PROSITE; PS51464; SIS; 2.
PE 3: Inferred from homology;
KW Aminotransferase; Complete proteome; Cytoplasm;
KW Glutamine amidotransferase; Repeat; Transferase.
FT INIT_MET 1 1 Removed (By similarity).
FT CHAIN 2 598 Glutamine--fructose-6-phosphate
FT aminotransferase [isomerizing].
FT /FTId=PRO_0000135431.
FT DOMAIN 2 219 Glutamine amidotransferase type-2.
FT DOMAIN 280 420 SIS 1.
FT DOMAIN 449 588 SIS 2.
FT ACT_SITE 2 2 Nucleophile; for GATase activity (By
FT similarity).
FT ACT_SITE 593 593 For Fru-6P isomerization activity (By
FT similarity).
SQ SEQUENCE 598 AA; 66110 MW; 3A8DD0466416544D CRC64;
MCGIIGYIGP RKASDVIVEG LKRLEYRGYD SAGIATCYEG KIFIKKGAGK IDELVKKLNF
LELPGNIGIG HTRWATHGIP NDTNAHPHTD CTGKIVVVHN GIIENFQELK RELLKRGHVF
RSDTDTEVIA HLIEENLRIT GNFEDAFRMS LLRLRGSYAL VVLFADDPER LYIARKDSPL
IIGIGKGEMF MASDIPAFLA YTRRAVFLDD GEYGIVSKDW FTIKDIITGA VKTKEIHEIQ
WTLEMAEKGG YEHFMLKEIF EQPKAIKDAI YGNVKEAPKV AELLMKYDRI IITGMGTSYH
AALVGKYLIQ RFGKVPVIVE EASELRYEYE DILDNRSLLI AITQSGETAD TVAAMKLAKS
KGVEVVGIVN VVGSLATRIA DETLYTHAGP EIGVAATKTY TTQLVVLTLL AKELGKLVGI
DVSQIEGTIP RLPELVDSSL KINDKIREIA VKLNDKRDFF YIGRGINYPT ALEGALKIKE
IAYVHAEGLS AGELKHGPLA LIEDGIPVVG IAPTGKTFEK MLSNIEEAKA RGGFIISLGD
DVRLHQVSDI FIRLPKVPEE LAPITYIVPL QLLAYHLAVL KGHNPDRPRN LAKSVTVE
//
