ID GEM1_SCHPO Reviewed; 630 AA.
AC O59781;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 01-MAY-2013, entry version 85.
DE RecName: Full=Mitochondrial Rho GTPase 1;
DE EC=3.6.5.-;
DE AltName: Full=GTPase EF-hand protein of mitochondria 1;
GN Name=gem1; ORFNames=SPCC320.04c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales;
OC Schizosaccharomycetaceae; Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Mitochondrial GTPase involved in mitochondrial
CC trafficking. Probably involved in control of anterograde transport
CC of mitochondria and their subcellular distribution (By
CC similarity).
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass
CC type IV membrane protein (By similarity).
CC -!- SIMILARITY: Belongs to the mitochondrial Rho GTPase family.
CC -!- SIMILARITY: Contains 2 EF-hand domains.
CC -!- SIMILARITY: Contains 2 Miro domains.
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DR EMBL; CU329672; CAA18306.1; -; Genomic_DNA.
DR PIR; T41307; T41307.
DR RefSeq; NP_587725.1; NM_001022720.2.
DR ProteinModelPortal; O59781; -.
DR STRING; 4896.SPCC320.04c-1; -.
DR EnsemblFungi; SPCC320.04c.1; SPCC320.04c.1:pep; SPCC320.04c.
DR GeneID; 2538910; -.
DR KEGG; spo:SPCC320.04c; -.
DR PomBase; SPCC320.04c; -.
DR eggNOG; COG1100; -.
DR HOGENOM; HOG000215553; -.
DR KO; K07870; -.
DR OMA; YERIALY; -.
DR OrthoDB; EOG4NCQMX; -.
DR NextBio; 20800088; -.
DR GO; GO:0005829; C:cytosol; IDA:PomBase.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0005741; C:mitochondrial outer membrane; ISO:PomBase.
DR GO; GO:0005635; C:nuclear envelope; IDA:PomBase.
DR GO; GO:0005509; F:calcium ion binding; ISM:PomBase.
DR GO; GO:0005525; F:GTP binding; IC:PomBase.
DR GO; GO:0003924; F:GTPase activity; ISO:PomBase.
DR GO; GO:0019725; P:cellular homeostasis; IEA:InterPro.
DR GO; GO:0007005; P:mitochondrion organization; ISO:PomBase.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IC:PomBase.
DR Gene3D; 1.10.238.10; -; 2.
DR InterPro; IPR011992; EF-hand-like_dom.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR013566; EF_hand_assoc_1.
DR InterPro; IPR013567; EF_hand_assoc_2.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR020860; MIRO.
DR InterPro; IPR013684; MIRO-like.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR021181; Small_GTPase_Miro.
DR PANTHER; PTHR24072:SF69; PTHR24072:SF69; 1.
DR Pfam; PF08355; EF_assoc_1; 1.
DR Pfam; PF08356; EF_assoc_2; 1.
DR Pfam; PF13405; EF_hand_4; 1.
DR Pfam; PF08477; Miro; 2.
DR PIRSF; PIRSF037488; Mt_Rho_GTPase; 1.
DR PRINTS; PR00449; RASTRNSFRMNG.
DR SMART; SM00054; EFh; 2.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
DR PROSITE; PS51423; MIRO; 2.
PE 3: Inferred from homology;
KW Calcium; Complete proteome; GTP-binding; Hydrolase; Membrane;
KW Metal-binding; Mitochondrion; Mitochondrion outer membrane;
KW Nucleotide-binding; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1 630 Mitochondrial Rho GTPase 1.
FT /FTId=PRO_0000239339.
FT TOPO_DOM 1 598 Mitochondrial intermembrane (Potential).
FT TRANSMEM 599 619 Helical; Anchor for type IV membrane
FT protein; (Potential).
FT TOPO_DOM 620 630 Cytoplasmic (Potential).
FT DOMAIN 1 155 Miro 1.
FT DOMAIN 184 219 EF-hand 1.
FT DOMAIN 304 339 EF-hand 2.
FT DOMAIN 415 561 Miro 2.
FT NP_BIND 10 17 GTP 1 (Potential).
FT NP_BIND 57 61 GTP 1 (Potential).
FT NP_BIND 113 116 GTP 1 (Potential).
FT CA_BIND 197 208 1 (Potential).
FT CA_BIND 317 328 2 (Potential).
FT NP_BIND 428 435 GTP 2 (Potential).
FT NP_BIND 459 463 GTP 2 (Potential).
FT NP_BIND 527 530 GTP 2 (Potential).
SQ SEQUENCE 630 AA; 71177 MW; 9B36A65260CA3919 CRC64;
MKEVRVVICG DQGVGKSSLI SALIQEDNVT SIPKVFPIIS IPSNPDSNDD VSLVLVDTQS
DSNEREYLAA EIKKANVICL VYSDNYSYER VSIFWLPYFR SLGVNVPIVL CENKSEDLDN
YQGLHTIEHE MIPLINEFKE IEACILCSAL EKINVNELFY MCRACVIYPI TPLWDAKERT
MRKATIHALS RIFFLIDKNN DDLLSVDELN SLSEKCFSKN LSIEDASEIL SKVKEICPEG
VYEGQLTLPG FLAYNRVQVE NGKQESTWGI LRAFHYTDSL SLDDSYLSPK FEVAPGQIVE
LSPKGYRFLV DLFYQFDRDN DGALNNEELS ALFRHTPGLP EIWVSSQFPN STVLNEHGYV
TYNGWLAQWS MITLFDYKTT LAYLAYLGFD TDGRGHNTDA LKVMRKRVSQ NRKVSKYDRN
VFLCFVVGSK SCGKTALLSS FINNNTNRLT PNTVVNSVEF QSTQRYLVLS EIGETDLDIL
AEPKSLEACD ILCLLYDSSN PNSFSFIANL LNLYPDLQKI PCVFAATKAD LDRQQQRYPV
QPDEFTKQLG LPSPTHISTA AIWNTSKEFF IQLAESAQYP ASSIIRIPEE DSNKTNYQLV
AALTAFGALL LSVGGSLTWK IIKHQYYSKK
//
