ID C71B9_ARATH Reviewed; 500 AA.
AC O64718; Q8VYE9;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 02-AUG-2002, sequence version 3.
DT 27-MAR-2024, entry version 153.
DE RecName: Full=Cytochrome P450 71B9;
DE EC=1.14.-.-;
GN Name=CYP71B9; OrderedLocusNames=At2g02580; ORFNames=T8K22.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AC004136; AAC18928.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC05601.1; -; Genomic_DNA.
DR EMBL; AY072124; AAL59946.1; -; mRNA.
DR PIR; T00605; T00605.
DR RefSeq; NP_178362.1; NM_126314.3.
DR AlphaFoldDB; O64718; -.
DR SMR; O64718; -.
DR STRING; 3702.O64718; -.
DR PaxDb; 3702-AT2G02580-1; -.
DR ProteomicsDB; 239200; -.
DR EnsemblPlants; AT2G02580.1; AT2G02580.1; AT2G02580.
DR GeneID; 814788; -.
DR Gramene; AT2G02580.1; AT2G02580.1; AT2G02580.
DR KEGG; ath:AT2G02580; -.
DR Araport; AT2G02580; -.
DR TAIR; AT2G02580; CYP71B9.
DR eggNOG; KOG0156; Eukaryota.
DR HOGENOM; CLU_001570_4_1_1; -.
DR InParanoid; O64718; -.
DR OMA; MNMEESP; -.
DR OrthoDB; 5393233at2759; -.
DR PhylomeDB; O64718; -.
DR BioCyc; ARA:AT2G02580-MONOMER; -.
DR PRO; PR:O64718; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O64718; baseline and differential.
DR Genevisible; O64718; AT.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR CDD; cd11072; CYP71-like; 1.
DR Gene3D; 1.10.630.10; Cytochrome P450; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR PANTHER; PTHR47956; CYTOCHROME P450 71B11-RELATED; 1.
DR PANTHER; PTHR47956:SF5; CYTOCHROME P450 71B25-RELATED; 1.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; Cytochrome P450; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..500
FT /note="Cytochrome P450 71B9"
FT /id="PRO_0000052087"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 440
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT CONFLICT 321
FT /note="P -> A (in Ref. 3; AAL59946)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 500 AA; 57084 MW; 9D23C396410E8E4E CRC64;
MATIWFLSLL FLCCILLAAF KHKKRRTNQQ QPPSPPGFPI IGNLHQLGEL PHQSLWSLSK
TYGPVMLLKL GSVPTVVVSS SETAKQVLKI NDLHCCSRPS LAGAKELSYN YLDIAFSPFD
DYWKELRRIC VQELFSAKRV HSIQPIKEEE VRKLIVSATE SASQKSPVNL SEKFLDLTVS
VICKAAFSLD FHTSVLNNDG FDKLIHDAFL FLGSFSASNF FPNGGWIIDW LTGLQRRREK
SVKDLDVFYQ QMFDLHKQEN KQGVEDFVDL LLKLEKEETV LGYGKLTRNH VKAILMNVLL
GAINTSAMTM TWAMAELIRN PRVMKKVQSE IRNQMINKSV ITLDDIDHLP YLKMVIKETW
RLHPPVPLLL PREVMSEFEI NGYKIQPKTL LYVNVWAIGR DPDSWKDADM FYPERFMDNN
IDAKGQNFEL LPFGSGRRIC PGMYMGTTMV EFGLANMLYQ FDWEVPDGMV VEDIDMEESP
GLAVGKKNEL LLVPVKYLGH
//
