ID O66590_AQUAE Unreviewed; 666 AA.
AC O66590;
DT 01-AUG-1998, integrated into UniProtKB/TrEMBL.
DT 01-AUG-1998, sequence version 1.
DT 27-MAR-2024, entry version 127.
DE SubName: Full=Nitrate reductase narB {ECO:0000313|EMBL:AAC06542.1};
GN Name=narB {ECO:0000313|EMBL:AAC06542.1};
GN OrderedLocusNames=aq_217 {ECO:0000313|EMBL:AAC06542.1};
OS Aquifex aeolicus (strain VF5).
OC Bacteria; Aquificota; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX NCBI_TaxID=224324 {ECO:0000313|EMBL:AAC06542.1, ECO:0000313|Proteomes:UP000000798};
RN [1] {ECO:0000313|EMBL:AAC06542.1, ECO:0000313|Proteomes:UP000000798}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VF5 {ECO:0000313|EMBL:AAC06542.1,
RC ECO:0000313|Proteomes:UP000000798};
RX PubMed=9537320; DOI=10.1038/32831;
RA Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA Feldman R.A., Short J.M., Olson G.J., Swanson R.V.;
RT "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL Nature 392:353-358(1998).
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Nitrogen metabolism. {ECO:0000256|ARBA:ARBA00004909}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. NasA/NapA/NarB subfamily.
CC {ECO:0000256|ARBA:ARBA00008747}.
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DR EMBL; AE000657; AAC06542.1; -; Genomic_DNA.
DR PIR; B70320; B70320.
DR RefSeq; NP_213150.1; NC_000918.1.
DR RefSeq; WP_010880088.1; NC_000918.1.
DR AlphaFoldDB; O66590; -.
DR SMR; O66590; -.
DR STRING; 224324.aq_217; -.
DR EnsemblBacteria; AAC06542; AAC06542; aq_217.
DR KEGG; aae:aq_217; -.
DR PATRIC; fig|224324.8.peg.181; -.
DR eggNOG; COG0243; Bacteria.
DR HOGENOM; CLU_000422_13_4_0; -.
DR InParanoid; O66590; -.
DR OrthoDB; 9789468at2; -.
DR Proteomes; UP000000798; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR CDD; cd02791; MopB_CT_Nitrate-R-NapA-like; 1.
DR CDD; cd02754; MopB_Nitrate-R-NapA-like; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.40.50.740; -; 1.
DR Gene3D; 2.20.25.90; ADC-like domains; 1.
DR Gene3D; 3.40.228.10; Dimethylsulfoxide Reductase, domain 2; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR041957; CT_Nitrate-R-NapA-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR PANTHER; PTHR43105:SF9; NITRATE REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G15190)-RELATED; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR Pfam; PF04879; Molybdop_Fe4S4; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Molybdenum {ECO:0000256|ARBA:ARBA00022505};
KW Nitrate assimilation {ECO:0000256|ARBA:ARBA00023063};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000000798}.
FT DOMAIN 1..49
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|SMART:SM00926"
SQ SEQUENCE 666 AA; 75404 MW; 797FFBDD9A643454 CRC64;
MKRFQCPYCG VGCGLYINEK GKVKGDFEHP ANLGDICKKP LYLPKVLNRG RIAKPMYREN
KSHSFREISW EEAYEILKEK LISFSPDETY FYLSGQLLTE DIYVANKFVK GFLRTNNVDA
NSRLCMASAV TAYKLAFGSD GVPCTYEDVD DADAFLFIGS NAAVAHPVLF KRVLKRRKEE
EKVIIVTVDP LETETAKRSD VFIQIKAGTD TVFLNSVLYV LHKEGWIDYR FIYNYTEGFE
EALEVAMKFP PDVAASICSV KPEDVYLVAE LFAHSKKLIS FWCQGLNQSL NGTMKNLALI
NLHLATGRLN EKGCPFSLTG QPNAMGGREV GYLTNGLPGY RDVRNKADRA FMEKFWGVKG
IKKEPGPTIT EAIDMILKDR IKFLWVVCTN PAVSLPNLEK VKRALEKVFL VVQDAYWNDT
CEFANLILPA AQLGEKEGVM TGSDRTVTFC ERFSEPFGES KPDWLIFTEL ARKMGAEELF
PYSSPKEIFE EFKASTKGRL CDMSNLSYEN LPARWGKRWL YEDLKFPTES GKARFHPARV
DVAAEEGMFI LLTGRLKNNW HTMTRTGKSP ELLKGEIPPF VIMNPRDAEE LGIEEGEEVI
LSAKGKEITR VVRFGNVKRG HIFAPFGYPS EYGEPTNLLT SDRTDPYSKE PDLKYSGVDV
FVKVNR
//
