ID BACC_BACLI Reviewed; 6359 AA.
AC O68008;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-APR-2013, entry version 83.
DE RecName: Full=Bacitracin synthase 3;
DE Short=BA3;
DE Includes:
DE RecName: Full=ATP-dependent isoleucine adenylase;
DE Short=IleA;
DE AltName: Full=Isoleucine activase;
DE Includes:
DE RecName: Full=ATP-dependent D-phenylalanine adenylase;
DE Short=D-PheA;
DE AltName: Full=D-phenylalanine activase;
DE Includes:
DE RecName: Full=ATP-dependent histidine adenylase;
DE Short=HisA;
DE AltName: Full=Histidine activase;
DE Includes:
DE RecName: Full=ATP-dependent D-aspartate adenylase;
DE Short=D-AspA;
DE AltName: Full=D-aspartate activase;
DE Includes:
DE RecName: Full=ATP-dependent asparagine adenylase;
DE Short=AsnA;
DE AltName: Full=Asparagine activase;
DE Includes:
DE RecName: Full=Aspartate racemase;
DE EC=5.1.1.13;
DE Includes:
DE RecName: Full=Phenylalanine racemase [ATP hydrolyzing];
DE EC=5.1.1.11;
GN Name=bacC;
OS Bacillus licheniformis.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=1402;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 10716 / DSM 603 / NBRC 12199 / NCIMB 8874;
RX PubMed=9427658; DOI=10.1016/S1074-5521(97)90301-X;
RA Konz D., Klens A., Schoergendorfer K., Marahiel M.A.;
RT "The bacitracin biosynthesis operon of Bacillus licheniformis ATCC
RT 10716: molecular characterization of three multi-modular peptide
RT synthetases.";
RL Chem. Biol. 4:927-937(1997).
CC -!- FUNCTION: Induces peptide synthesis, activates and incorporates
CC five amino acids, forms a thiazoline ring between the first two
CC amino acids and incoporates a D-glutamine in the fourth position.
CC -!- CATALYTIC ACTIVITY: L-aspartate = D-aspartate.
CC -!- CATALYTIC ACTIVITY: ATP + L-phenylalanine + H(2)O = AMP +
CC diphosphate + D-phenylalanine.
CC -!- COFACTOR: Binds 5 phosphopantetheines covalently (Potential).
CC -!- PATHWAY: Antibiotic biosynthesis; bacitracin biosynthesis.
CC -!- SUBUNIT: Large multienzyme complex of BA1, BA2 and BA3.
CC -!- DOMAIN: Consists of five modules with two epimerization domains in
CC the second and fourth modules, and a putative C-terminal
CC thioesterase domain. Each module incorporates one amino acid into
CC the peptide product and can be further subdivided into domains
CC responsible for substrate adenylation, thiolation, condensation
CC (not for the initiation module), and epimerization (optional), and
CC N methylation (optional).
CC -!- MISCELLANEOUS: Bacitracin is a mixture of at least ten cyclic
CC dodecapeptides, that differ by one or two amino acids. The most
CC abundant is bacitracin A, a branched cyclic dodecapeptide. It
CC contains an N-terminal linear pentapeptide moiety (Ile-Cys-Leu-D-
CC Glu-Ile) with an isoleucine-cysteine thiazoline condensation
CC product and a C-terminal heptapeptide ring (Lys-D-Orn-Ile-D-Phe-
CC His-D-Asp-Asn), in which the free alpha-carboxy group of the C-
CC terminal Asn is bound to the epsilon-amino group of Lys.
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme
CC family.
CC -!- SIMILARITY: Contains 5 acyl carrier domains.
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DR EMBL; AF007865; AAC06348.1; -; Genomic_DNA.
DR PIR; T31679; T31679.
DR ProteinModelPortal; O68008; -.
DR UniPathway; UPA00179; -.
DR GO; GO:0047689; F:aspartate racemase activity; IEA:EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0047462; F:phenylalanine racemase (ATP-hydrolyzing) activity; IEA:EC.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1200.10; -; 5.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR009081; Acyl_carrier_prot-like.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR010060; NRPS_synth.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR006162; PPantetheine_attach_site.
DR InterPro; IPR001031; Thioesterase.
DR Pfam; PF00501; AMP-binding; 5.
DR Pfam; PF00668; Condensation; 7.
DR Pfam; PF00550; PP-binding; 5.
DR Pfam; PF00975; Thioesterase; 1.
DR SMART; SM00823; PKS_PP; 5.
DR SUPFAM; SSF47336; ACP_like; 5.
DR TIGRFAMs; TIGR01733; AA-adenyl-dom; 5.
DR TIGRFAMs; TIGR01720; NRPS-para261; 2.
DR PROSITE; PS50075; ACP_DOMAIN; 5.
DR PROSITE; PS00455; AMP_BINDING; 5.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 4.
PE 3: Inferred from homology;
KW Antibiotic biosynthesis; ATP-binding; Hydrolase; Isomerase; Ligase;
KW Multifunctional enzyme; Nucleotide-binding; Phosphopantetheine;
KW Repeat.
FT CHAIN 1 6359 Bacitracin synthase 3.
FT /FTId=PRO_0000193084.
FT DOMAIN 966 1033 Acyl carrier 1.
FT DOMAIN 1998 2064 Acyl carrier 2.
FT DOMAIN 3502 3569 Acyl carrier 3.
FT DOMAIN 4544 4610 Acyl carrier 4.
FT DOMAIN 6052 6119 Acyl carrier 5.
FT REGION 461 1034 Domain 1 (isoleucine-activating).
FT REGION 1517 2064 Domain 2 (D-phenylalanine-activating).
FT REGION 2999 3570 Domain 3 (histidine-activating).
FT REGION 4047 4612 Domain 4 (D-aspartic acid-activating).
FT REGION 5549 6129 Domain 5 (asparagine-activating).
FT MOD_RES 996 996 O-(pantetheine 4'-phosphoryl)serine (By
FT similarity).
FT MOD_RES 2028 2028 O-(pantetheine 4'-phosphoryl)serine (By
FT similarity).
FT MOD_RES 3532 3532 O-(pantetheine 4'-phosphoryl)serine (By
FT similarity).
FT MOD_RES 4574 4574 O-(pantetheine 4'-phosphoryl)serine (By
FT similarity).
FT MOD_RES 6082 6082 O-(pantetheine 4'-phosphoryl)serine (By
FT similarity).
SQ SEQUENCE 6359 AA; 722943 MW; 82A273C546253074 CRC64;
MKTKVEKIYP LSNMQKGMLF HAMKDEASHA YFEQFIIELK GDVDERMFEE SLNEVMKRHE
ILRASFHHRL DEPLHVIIKD RHMKFDYLDI RGRHDQDGVL ERYLAEDRQK GFDLAKDTLM
RACLIRMSDD SYQFVWTYHH ILLDGWCLGI ILDELLTIYE MKRKGQNHQL EDPRPYSDYI
KWLEDQDKEE AQSYWESYLS GYDQKNSLPK LRTPSETGFK RREKTIECSK ELTNRLIKLA
NRNHVTINTV LQSIWGVILA KYNNSEDVVF GTVVSGRDAE VEGIETMVGV FINTIPTRIR
LDKDKLFKDV LRQTQADALE SSRYNYMNLA EVQALSELKN DLIDHVMVFE NYAVDQKAFE
EKNDVGFEMV NVSGEEQTNY HFSISAALDD QLKLLFIYDE NVYDTTIIET LEKHIITVAE
QVAEDETQTL RDINLVSKEE QHRILDTFND TKTGYPKDKP LHELFEEQAM KTPDHTALVF
GAQRMTYREL NEKANQTARL LREKGIGRGS IAAIIADRSF EMIIGIIGIL KAGGAYLPID
PETPKHRIAF MLSDTKAGVL LAQGKAADGI DCEADIIHLD KGVADGFSKK RLSSVNDSGD
TAYIIYTSGS TGMPKGVVTP HYSAARVVKN TNYIDITEDD AILQLSNYSF DGSVFDIFGA
LLNGASLVLI EKETVLNTHE LAEVIKKEQV SVMFITTALF NTLADINIGC LAKLRKIFLG
GERASIPHVR KVLNHVGRDK LIHVYGPTES TVYATYYFIN EIDDEAETIP IGSPLANTSV
LIMDEAGKLL PIGVPGELCI AGDGLSKGYL NREELTAEKF IPHPFIPGER LYKTGDLAKW
LPDGNIEFIG RIDHQVKIRG FRIELGEIES RLEMHEDINE TIVTVREDEE SRPYICAYIT
ANREISLDEL KGFLGEKLPE YMIPAYFVKM DKLPLTKNGK VDRKALPEPD RTAGAENEYE
APRNETEEKL AAIWRDILKV EKSGINDHFF EMGGHSLKAA AMAARIRKEL KAEIPLGQIF
KTPTIKGLGE YIRSTKDSVY SSIQKVEEKE YYRLSSAQKR LYILDQIEGS GLSYNIPFTM
KVKGRFDIRR FENALKTIIQ RHEALRTSFL MADGEPVQKI EKEVDFSIKC SKIQSLSIQE
IIKQFVRPFD LKKAPLFRTE VVKVDDEEHI ILFDMHHIIS DGASMGVLTK EICDLYGGKE
LKPLSLQYKD YSEWQRDFYQ KDEMKRQKEY WLNIFKGEIP VLNMPTDYPR PQMHSVEGDR
IGFAIDGELT KKLKRIAKDN GATMYMLLLA AYTVLLRTYS GQEDVIIGTP IQGRKHHELK
HVIGMFVNTL AMRNHPKGDK TFAEYLQDVK ETALKAYENQ DYQFDDLVEQ LDLERDMSRN
PLFDTMFVLQ NLEKADAEIE GLTFEPFESD IHISKFDLTL SAIEKDSKIE FDLEYCTKLF
KRETVERMAA HFVRVLEDIS KRTDKRLDQI EAMSEDEKNT LLYRFNDTKT DAPTDKTICQ
LFAERAETSP DKTAVVFEDQ TLTYRQLHER SNQLARFLRE KGVQPDTAVG IMVDRSPEMI
IGLLGILKAG GAYLPLDPAY PEDRIKYILG DSQTKFLLSE EALIKKRAFI KEADMINIDI
HDKQIAAQDA AQLEPVSRSG DLAYIIYTSG STGKPKGVLI EQKGLSNLVS AVVKLMHLNT
GSRVIQFASL SFDASAFEIF PALAAGSALV LGRQEEMMPG QPLTSFLRQY NITHATLPPT
VLDVLNESGL ENLKVIVSAG SACSEELAKR WSGNRLFINA YGPTETTVCA TAGIYEGSGR
PHIGSPIANT NVYVLDQNQK PVPTGVVGEL CVGGMSLARG YLNRPELTAE KFISHPFASG
ERLYRTGDLA RWLPDGHLEF LGRIDHQVKI RGYRIELGEI ENQLLKLDKI DEAAVIARKD
DDHSDYLCAY IVSKEDWTST EISEWLEKEL PHYMIPAYFV RLDKLPLTSN DKVDRKALPA
PDRHVATGAV YEAPRNDTEA KLVDIWRDVL GAGDIGISHH FFAAGGDSIK ALQIVSRLSR
LGLKLEMKDL FANPRIKDLA KYVKKQSQRK NANTIVTGHA ELTPIQKWYF ANNKEELDHF
NQSFVLFRKG GFDESCVKKA FNKIMEQHDA LRMIYEEKGG DFIQYNRSFR EDLFDLDVYD
VRGLDRQAEK VYELATSIQK LSSIRKGKLV HLGIFRADEG DHLLIVIHHL VVDGVSWRIL
FEDFETLYSQ ALKGQTLEIG YKTDSYQEFA RRLKAYAHSR TLSKEAEYWR NIAKARVRFI
PPKNVLKEDV YENSTTLSIK LGKEATADLL RNTNRAYNTE INDILLTALL TGARDITGEN
KLKVMMEGHG REDILEGVDI TRTIGWFTTM YPVLLDAGEE KALSQQIKMV KETLRKIPNK
GIGYGLLKYM AEDPDFTNEE KARISFNYLG DIDADMNRGE FSGSSFSEGE SIGGKIARSH
SIEINAIVMN HELVIHTTFN QMEYEKDTIS RLNHQLKERL EQIIKHCTQQ TESERTPSDY
GDTNISLAEL EEIKGKYRSA IEKIYPLANM QKGMLFHAIE DHTSDAYFQQ TVMDIEGYVD
PAILEASFND IMKRHEILRA SYEYEIVEEP RQIIIENRSI DFTYFNIAKS SAQQQEMFIE
RLLNEDRKKG FDLSKDVLMR AYLLKTAERS YRLVWSHHHI LLDGWCLGII MRELFVIYEN
RMNGKASPLK ETKPYSDYIK WLERQDQEEA RQYWREYLKG YEEQAQLPTL TKRKKSSRYD
RREKVIHLSK QLTKQLKELA AKNSVTLHTV IQTIWGLMLT RYTKIDDVVF GTVVSGREAN
VDGIEDMIGL FINTIPTRIR FNEQARFNDC LQKVQEDAIQ SNRYNYMNLA EVQALSSLKK
DLIDHILVFE NYEADEQDFE ESQMKTGFKV NEISAAEQSI TAFSMSVTPG EELTLVLTYD
GNVYDRDIIN NIEGHIKRVA EQVTANENRK IAEIDMLAEE ERKTLLYEFN RTNADYPRNK
TIHQLFEEQA ERTPGHTAVV FEKEELSYKA LNERSNQLAG LLREKGVKPD MIVGVMAERS
VEMIVGMLAV LKAGGAYLPI DPEYPEDRIR YMIEDSGISI LLKKADKQID VDFTCIDMNE
KGLAKDMAAE NLGHTSGSSD MAYVIYTSGS TGKPKGVMVN HQSIVNTLYW RKQSYGYSTA
DATLQVPSFS FDSSVEDIFT TLISGAKLVL IRDLRMNPRE IIGVLRTHKA TNLLAVPSFY
LNLLDTIEQP LDDLRFVTVA GEGFNESLIR QHFEKLPNVK LFNEYGPTEN SVCSTRGELR
KDDEKVVIGR PISNHKVYIL NHNQQLLPLG TPGELCLSGE GLARGYLNRP DLTLEKFVPN
PFAPGESMYR TGDLARFLPD GQIEYLGRID HQVKIRGFRI ELGEIENQLL KIEGIDAAAV
MAREDQAGGK YLCAYIVADK AAGVADVRKC LLKELPDYMV PSYFVKLDQL PLTANGKIDR
KALPEPSSTI SEATYEAPRN RTEEKLVSIW EDVLGIENIG ISHNFFELGG HSLKAAALTA
KLHKEMKIEV PLRQLFETPT IKDIGDFIES MKESPYASIT QAEEKEYYAL SSAQRRLYIL
NQIEPGGLSY NMPFAMKIAG DFDVDRFEDA FRQLIERHEA LRTAFVMVDG EPVQKIEKEV
DFKVKYGRLG QDPLEEKIKA FIKPFALEKA PLLRAEVLKA SGDEHVLMLD MHHIISDGVS
MAIFTRELAE LYEGKTLPPL TIQYKDFSEW QKLFYQKDEV KRQEDYWLNV FQGEVPVLNL
PADEKRPQKR SIEGDIVQFE IDGETSAMLN KLAKENGATM YMLLLAGYTT LLAKYTGQED
IVVGSPIAGR HHSDLKHVIG LFINTLAMRN HPKGDMPFAD YLKEVKETAL KAYENQDYPF
DELVEKLDVK RDMSRHPLFD TMLVLQNFDG DEADIDGLTF QPLQTEVNIS KFDLTLTAAE
TNEGIQCVFN YSTKLFKRST IERMAGHLIN ILKEAANDPH MPLSDVNMLS DEEMNALLDQ
NQGKQADYPQ DQTVHQLFEQ QADKTPEQTA VVYADEKLTY RELNERANQL ARLLRDKGAD
ADQPVAIMIE PSLEMIISML AVLKAGAAYV PIEPEQLAKR TNEILSDSRA AILLVKGSVK
ENVAFAGEIV NVADGLIDAK VASNLSASGS ADQNAYIIYT SGSTGKPKGV FVRHGNVVNY
TTWFMKEAGL TENDKAMLVS SYAFDLGYTS IFSALLSGSE LHIARKECYT NAHRALKYIK
ENGITYIKLT PSLFNIFVND PGFSAEKPCA TLRLVVLGGE MINTRDVETF YNQYPDHVVM
NHYGPTETTI GSVFKVIDPE HLDSFKECPV IGTPIHNTNA YVLDENMKLL PEGVYGELCI
AGAGVTGGYV NRPDETKEKF IENPFAPHTK MYRTGDLARR LSDGNIELAG RIDTQVKVRG
YRIEPEEIKN RLLAHDDIKE AFIAAREDHK GAKQLCAYFT ADAELPFEDI RTYLMHELPE
YMIPSSFVQI EKMPLSANGK IDTAALPEPQ PGKETEYEPP RNETEEKLVQ IWEEVLGIDK
IGITHHFFAA GGDSIKALQM ISRLSREGLS LEMKDLFANP QIKSLSRYVK AESDKSASYE
TVEGEVLLTP IQQEYFSLNK TDRNHYNHAV MLYRKNGFDE SIVKRVFKEI IKHHDALRTV
FTEEDGKIIQ YNRGPDKQLF DLFVYDVSSE NDQPQKVYQL ATELQQSIDI ETGPLVKLAV
FKTNNGDHLL IIIHHLVVDG ISWRILFEDL AIGYSQLANG EKVEFYPKTA SYQAYARHIA
EYAKSVKLLS EKQYWLKAIA EGVEFLDMNE NAGAFKVEDS RTFSTELEKE ETKRLLRETN
RAYHTEINDI LITALLVAAR DMNGQNQLRI TLEGHGREQV ADGIDISRTV GWFTSKYPVF
IDLGQETDMS RTIKMVKEHL RNVPNKGIGY GILKYLTRDS EIAKGAASPI LFNYLGQLDE
DINSGEFSSS HLSPGEAAGK GITREHPLEI NAVVFRGKLA IQTTYNTRAY SEDVVRAFAQ
NYKEALKAVI RHCAEREETE KTPSDYGDKG ISLDQLEEIK LKYKGMEIEK IYPLANMQRG
MLFHALEDKE SQAYFEQMAI NMKGLIDERL FAETFNDIME RHEILRASIE YEITDEPRNV
IIKDRKINLD YHDLRKQSPA EREQVIQAYR KADREKGFRL NSEPLIRAAL MRTEDDSYTF
IWTNHHILLD GWSRGIIMGE LFHMYHMKEA RQKHRLEEAR PYSDYIGWLQ QQDKEAAKAY
WRNYLSGFTE KSPISVLAGS SGHAKYKRKE AVIEFPEQLT GRITELASRN NVTFHTVLQC
IWGMLLARYN QTDDVVFGTV ISGRDAQVTG IEKMVGLFIN TVPTRIRLDK SQSFKELIKS
VQEQALEGRT YHDMNLSEVQ SLSELKRELL DHILIFENYA VDQSAFETSG KRGAGFVFEE
IHAEEQTNYG FNIVAVPGER LVIKLTYDGN IYHDHIIAGI KGHLQQVMEQ VVQHEDQSLN
DITVLSEAER NRLLYEWNDT KAEYPNQTIH RLFEEQAEKT PELAAVVSGN DKLTYRELNE
KSNQLARYLR DKGVKADTIV AIMAERSPEM VVGIMGILKA GGAYLPIDPD YPEERIKYML
EDSGAAIILA DHKQDLGTLH QEAVELTGDF SSYPADNLEP AGNADSLAYI IYTSGSTGKP
KGVMIRQRGL VNYITWADRV YVQGEQLDFA LYSSIAFDLT VTSIFTPLIS GNRVIVYRHS
EDGEPLIRKV FRDQKAGIVK LTPSHLSLVK DMDASGSSIK RLIVGGEDLK TELAKEITER
FHHNIEIYNE YGPTETVVGC MIYQYDAGWD RQVSVPIGKP ASNVQLYILD ERQEVQPVGI
AGELYISGDG VAKGYLNKPE LTSEKFLPNP FLPGERMYRT GDLAKMRPDG HIEYLGRIDH
QVKIRGYRIE LGEIEHQLLR HSDIKEAAVA AKTDQNNDQV LCAYVVSERD ITQKDIKTFL
AKELPEYMVP SYLLKLDELP LTPNGKVDLK ALPEPDRSAG ALLEYEPPRH ELEEKMAAIW
EDILNIEQIG INANIFDIGA NSLNVMSFVS RLYAELGFRV PFKDIFSKPT IKELSDFLKH
AQDLLKDYTD DCMQLTRAEE GGKNLFCFPP AASMGIAYMG LAKHLKQHSV YSFNFIPSAN
RIRKYADIIK NIQGEGPYTL IGYSSGGILA FDVAKELNRQ GYEVEDLIII DSKYRTKAEK
HQFTEEEYRE EISKTFELEK YRDVEKLLSD YLVDLVMKSY VYIQNTVTTG AIDGHISYIK
SSDNQRGENM MMWEKATSKT FTVVQGAGTH MQMISKSHPD ILERNARLIH DIINKTVKI
//
