ID KEN_DROME Reviewed; 601 AA.
AC O77459;
DT 02-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 01-MAY-2013, entry version 105.
DE RecName: Full=Transcription factor Ken;
DE AltName: Full=Protein Ken and Barbie;
GN Name=ken; ORFNames=CG5575;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
OC Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha;
OC Ephydroidea; Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=10349629; DOI=10.1016/S0925-4773(98)00183-X;
RA Kuehnlein R.P., Chen C.-K., Schuh R.;
RT "A transcription unit at the ken and barbie gene locus encodes a novel
RT Drosophila zinc finger protein.";
RL Mech. Dev. 79:161-164(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX PubMed=10191082; DOI=10.1006/geno.1999.5746;
RA Lukacsovich T., Asztalos Z., Juni N., Awano W., Yamamoto D.;
RT "The Drosophila melanogaster 60A chromosomal division is extremely
RT dense with functional genes: their sequences, genomic organization,
RT and expression.";
RL Genomics 57:43-56(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=14518006; DOI=10.1002/arch.10105;
RA Lukacsovich T., Yuge K., Awano W., Asztalos Z., Kondo S., Juni N.,
RA Yamamoto D.;
RT "The ken and barbie gene encoding a putative transcription factor with
RT a BTB domain and three zinc finger motifs functions in terminalia
RT development of Drosophila.";
RL Arch. Insect Biochem. Physiol. 54:77-94(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X.,
RA Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D.,
RA Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G.,
RA Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D.,
RA Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M.,
RA Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S.,
RA Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P.,
RA Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I.,
RA Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P.,
RA de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M.,
RA Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P.,
RA Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W.,
RA Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K.,
RA Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J.,
RA Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C.,
RA Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A.,
RA Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z.,
RA Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X.,
RA Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D.,
RA Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A.,
RA Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L.,
RA Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M.,
RA Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G.,
RA Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H.,
RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E.,
RA Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X.,
RA Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J.,
RA Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A.,
RA Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L.,
RA Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X.,
RA Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q.,
RA Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M.,
RA Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a
RT systematic review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M.,
RA George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H.,
RA Rubin G.M., Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=16401426; DOI=10.1016/j.cub.2005.11.033;
RA Arbouzova N.I., Bach E.A., Zeidler M.P.;
RT "Ken & barbie selectively regulates the expression of a subset of
RT Jak/STAT pathway target genes.";
RL Curr. Biol. 16:80-88(2006).
CC -!- FUNCTION: Transcription factor required for terminalia
CC development. Negative regulator of the JAK/STAT pathway: represses
CC JAK/STAT-dependent expression of ventral veins lacking (vvl) in
CC the posterior spiracles.
CC -!- SUBCELLULAR LOCATION: Nucleus (Probable).
CC -!- TISSUE SPECIFICITY: Expressed from stage 5 in two rather faint
CC stripes at positions of 64% (anterior domain; AD) and 17%
CC (posterior domain; PD) egg length. During early gastrulation, at
CC stage 6, these two stripes become more evident and detectable at
CC the region posterior to the cephalic furrow and in the hindgut
CC primordium. The AD disappears as gastrulation proceeds, while the
CC PD remains. At stage 15, the AD appears again in the foregut, and
CC PD expression in the hindgut and anal pad. In imaginal disks, it
CC is ubiquitously expressed in both males and females in genital and
CC eye-antennal disks. Not expressed in the brain. In genital disks,
CC it is expressed along the margin of the anterior bulbus in males,
CC while in females it is expressed in the posterior compartment
CC along the anterior-posterior border, with medial expansion in the
CC most posterior region.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Highly expressed in embryos and pupae, and at lower level in
CC larvae. In adults, it is expressed at higher level in females.
CC -!- DISRUPTION PHENOTYPE: Flies show low mating success and reduced
CC copulation duration. Men and female genitalia often remain inside
CC the body, and genitalia and analia are missing in some homozygous
CC flies. These flies are named after the famous dolls who also lack
CC these 'features'.
CC -!- SIMILARITY: Contains 1 BTB (POZ) domain.
CC -!- SIMILARITY: Contains 3 C2H2-type zinc fingers.
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DR EMBL; AJ012576; CAA10062.1; -; mRNA.
DR EMBL; AB010260; BAA32683.1; -; mRNA.
DR EMBL; AB010261; BAA32687.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF47084.1; -; Genomic_DNA.
DR EMBL; AF181647; AAD55433.1; -; mRNA.
DR PIR; T00119; T00119.
DR RefSeq; NP_523833.1; NM_079109.3.
DR UniGene; Dm.2792; -.
DR ProteinModelPortal; O77459; -.
DR SMR; O77459; 498-589.
DR DIP; DIP-22018N; -.
DR IntAct; O77459; 1.
DR MINT; MINT-981522; -.
DR STRING; 7227.FBpp0072038; -.
DR PaxDb; O77459; -.
DR PRIDE; O77459; -.
DR EnsemblMetazoa; FBtr0072129; FBpp0072038; FBgn0011236.
DR GeneID; 37785; -.
DR KEGG; dme:Dmel_CG5575; -.
DR CTD; 37785; -.
DR FlyBase; FBgn0011236; ken.
DR eggNOG; NOG330125; -.
DR GeneTree; ENSGT00680000099994; -.
DR InParanoid; O77459; -.
DR OMA; SPENYVV; -.
DR OrthoDB; EOG4R2294; -.
DR PhylomeDB; O77459; -.
DR GenomeRNAi; 37785; -.
DR NextBio; 805393; -.
DR Bgee; O77459; -.
DR GermOnline; CG5575; Drosophila melanogaster.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IMP:UniProtKB.
DR GO; GO:0003700; F:sequence-specific DNA binding transcription factor activity; IMP:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0045497; P:female analia development; IMP:UniProtKB.
DR GO; GO:0030540; P:female genitalia development; IMP:UniProtKB.
DR GO; GO:0007320; P:insemination; IMP:FlyBase.
DR GO; GO:0045496; P:male analia development; IMP:UniProtKB.
DR GO; GO:0030539; P:male genitalia development; IMP:UniProtKB.
DR GO; GO:0046426; P:negative regulation of JAK-STAT cascade; IMP:UniProtKB.
DR GO; GO:0006911; P:phagocytosis, engulfment; IMP:FlyBase.
DR GO; GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
DR Gene3D; 3.30.160.60; -; 2.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ-like.
DR InterPro; IPR011333; BTB/POZ_fold.
DR InterPro; IPR013069; BTB_POZ.
DR InterPro; IPR007087; Znf_C2H2.
DR InterPro; IPR015880; Znf_C2H2-like.
DR InterPro; IPR013087; Znf_C2H2/integrase_DNA-bd.
DR Pfam; PF00651; BTB; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF54695; BTB/POZ_fold; 1.
DR PROSITE; PS50097; BTB; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 2: Evidence at transcript level;
KW Complete proteome; Developmental protein; DNA-binding; Metal-binding;
KW Nucleus; Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1 601 Transcription factor Ken.
FT /FTId=PRO_0000046925.
FT DOMAIN 33 101 BTB.
FT ZN_FING 500 522 C2H2-type 1.
FT ZN_FING 528 551 C2H2-type 2.
FT ZN_FING 567 590 C2H2-type 3.
FT COMPBIAS 245 258 His-rich.
FT COMPBIAS 257 278 Asn-rich.
SQ SEQUENCE 601 AA; 67033 MW; C9AAA437DAD48FE8 CRC64;
MKEFQRMLML QYSKHGECIL KEIGAAFRGE HPADLTIVCE NKVKLHAHKL VLAAASPLIR
NLLEDTHLSD CSTTVYFPDV NATYFKFLLD FLYSGQTCIT SRDVNYLHDL LLLLQIKSDS
WKTTDSAYLS SKCGGLRDRA DRRKQQYTSP QNLEPDQTLK YEVDSVDESR NAADFSSAFN
SNDNCESAAE CERSGGHNNK EEDEDDCTHK DNKSDKDTDE IVNLSNAPPS GTSGSNSNIS
TSSNHQQQQH HHHHHHNHNN NNNNNNNNSS SSTINPVNLS LDLRTKSENS ASRTLGSGSD
HSGIDLAVTA SESTKRKGLF FDSHKDVMKP LSDGSDINSS PENYVVTPHR KRRPGFHNTQ
SDNQPFTSYP HSLLEELRLA KSTTSPISGF GSEKNMLAHL EDGALNGDTL TPDRKHLLEA
QRNRAQSPEI PMHLGPQFVY QWQSNQNAAM SAMPNLQSRL SSLSHISLNL DHPEGRSGSA
SGSGANLAGS NTHASSVREY RCEYCGKQFG MSWNLKTHLR VHTGEKPFAC RLCVAMFKQK
AHLLKHLCSV HRNVITTTNG ADTENRYSCC FCSMCFESVQ ELVRHLSGHH NNLLLTKNLR
E
//
