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Database: UniProt
Entry: O77459
LinkDB: O77459
Original site: O77459 
ID   KEN_DROME               Reviewed;         601 AA.
AC   O77459;
DT   02-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   26-NOV-2014, entry version 117.
DE   RecName: Full=Transcription factor Ken;
DE   AltName: Full=Protein Ken and Barbie;
GN   Name=ken; ORFNames=CG5575;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
OC   Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha;
OC   Ephydroidea; Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP   AND DISRUPTION PHENOTYPE.
RX   PubMed=10349629; DOI=10.1016/S0925-4773(98)00183-X;
RA   Kuehnlein R.P., Chen C.-K., Schuh R.;
RT   "A transcription unit at the ken and barbie gene locus encodes a novel
RT   Drosophila zinc finger protein.";
RL   Mech. Dev. 79:161-164(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX   PubMed=10191082; DOI=10.1006/geno.1999.5746;
RA   Lukacsovich T., Asztalos Z., Juni N., Awano W., Yamamoto D.;
RT   "The Drosophila melanogaster 60A chromosomal division is extremely
RT   dense with functional genes: their sequences, genomic organization,
RT   and expression.";
RL   Genomics 57:43-56(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, AND
RP   DEVELOPMENTAL STAGE.
RX   PubMed=14518006; DOI=10.1002/arch.10105;
RA   Lukacsovich T., Yuge K., Awano W., Asztalos Z., Kondo S., Juni N.,
RA   Yamamoto D.;
RT   "The ken and barbie gene encoding a putative transcription factor with
RT   a BTB domain and three zinc finger motifs functions in terminalia
RT   development of Drosophila.";
RL   Arch. Insect Biochem. Physiol. 54:77-94(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X.,
RA   Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D.,
RA   Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G.,
RA   Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D.,
RA   Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M.,
RA   Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S.,
RA   Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P.,
RA   Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I.,
RA   Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P.,
RA   de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M.,
RA   Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P.,
RA   Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W.,
RA   Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K.,
RA   Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J.,
RA   Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C.,
RA   Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A.,
RA   Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z.,
RA   Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X.,
RA   Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D.,
RA   Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A.,
RA   Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L.,
RA   Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M.,
RA   Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G.,
RA   Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H.,
RA   Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E.,
RA   Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X.,
RA   Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J.,
RA   Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A.,
RA   Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L.,
RA   Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X.,
RA   Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q.,
RA   Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M.,
RA   Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a
RT   systematic review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Head;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M.,
RA   George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H.,
RA   Rubin G.M., Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [7]
RP   FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX   PubMed=16401426; DOI=10.1016/j.cub.2005.11.033;
RA   Arbouzova N.I., Bach E.A., Zeidler M.P.;
RT   "Ken & barbie selectively regulates the expression of a subset of
RT   Jak/STAT pathway target genes.";
RL   Curr. Biol. 16:80-88(2006).
CC   -!- FUNCTION: Transcription factor required for terminalia
CC       development. Negative regulator of the JAK/STAT pathway: represses
CC       JAK/STAT-dependent expression of ventral veins lacking (vvl) in
CC       the posterior spiracles. {ECO:0000269|PubMed:14518006,
CC       ECO:0000269|PubMed:16401426}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed from stage 5 in two rather faint
CC       stripes at positions of 64% (anterior domain; AD) and 17%
CC       (posterior domain; PD) egg length. During early gastrulation, at
CC       stage 6, these two stripes become more evident and detectable at
CC       the region posterior to the cephalic furrow and in the hindgut
CC       primordium. The AD disappears as gastrulation proceeds, while the
CC       PD remains. At stage 15, the AD appears again in the foregut, and
CC       PD expression in the hindgut and anal pad. In imaginal disks, it
CC       is ubiquitously expressed in both males and females in genital and
CC       eye-antennal disks. Not expressed in the brain. In genital disks,
CC       it is expressed along the margin of the anterior bulbus in males,
CC       while in females it is expressed in the posterior compartment
CC       along the anterior-posterior border, with medial expansion in the
CC       most posterior region. {ECO:0000269|PubMed:10349629,
CC       ECO:0000269|PubMed:14518006, ECO:0000269|PubMed:16401426}.
CC   -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC       Highly expressed in embryos and pupae, and at lower level in
CC       larvae. In adults, it is expressed at higher level in females.
CC       {ECO:0000269|PubMed:10349629, ECO:0000269|PubMed:14518006,
CC       ECO:0000269|PubMed:16401426}.
CC   -!- DISRUPTION PHENOTYPE: Flies show low mating success and reduced
CC       copulation duration. Men and female genitalia often remain inside
CC       the body, and genitalia and analia are missing in some homozygous
CC       flies. These flies are named after the famous dolls who also lack
CC       these 'features'. {ECO:0000269|PubMed:10349629}.
CC   -!- SIMILARITY: Contains 1 BTB (POZ) domain. {ECO:0000255|PROSITE-
CC       ProRule:PRU00037}.
CC   -!- SIMILARITY: Contains 3 C2H2-type zinc fingers.
CC       {ECO:0000255|PROSITE-ProRule:PRU00042}.
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DR   EMBL; AJ012576; CAA10062.1; -; mRNA.
DR   EMBL; AB010260; BAA32683.1; -; mRNA.
DR   EMBL; AB010261; BAA32687.1; -; Genomic_DNA.
DR   EMBL; AE013599; AAF47084.1; -; Genomic_DNA.
DR   EMBL; AF181647; AAD55433.1; -; mRNA.
DR   PIR; T00119; T00119.
DR   RefSeq; NP_523833.1; NM_079109.3.
DR   UniGene; Dm.2792; -.
DR   ProteinModelPortal; O77459; -.
DR   SMR; O77459; 498-589.
DR   BioGrid; 63378; 3.
DR   DIP; DIP-22018N; -.
DR   IntAct; O77459; 1.
DR   MINT; MINT-981522; -.
DR   STRING; 7227.FBpp0072038; -.
DR   PaxDb; O77459; -.
DR   PRIDE; O77459; -.
DR   EnsemblMetazoa; FBtr0072129; FBpp0072038; FBgn0011236.
DR   GeneID; 37785; -.
DR   KEGG; dme:Dmel_CG5575; -.
DR   CTD; 37785; -.
DR   FlyBase; FBgn0011236; ken.
DR   eggNOG; NOG330125; -.
DR   GeneTree; ENSGT00740000115517; -.
DR   InParanoid; O77459; -.
DR   OMA; SPENYVV; -.
DR   OrthoDB; EOG771273; -.
DR   PhylomeDB; O77459; -.
DR   GenomeRNAi; 37785; -.
DR   NextBio; 805393; -.
DR   PRO; PR:O77459; -.
DR   Bgee; O77459; -.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IMP:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:sequence-specific DNA binding transcription factor activity; IMP:UniProtKB.
DR   GO; GO:0007620; P:copulation; NAS:FlyBase.
DR   GO; GO:0045497; P:female analia development; IMP:UniProtKB.
DR   GO; GO:0030540; P:female genitalia development; IMP:UniProtKB.
DR   GO; GO:0007320; P:insemination; IMP:FlyBase.
DR   GO; GO:0045496; P:male analia development; IMP:UniProtKB.
DR   GO; GO:0030539; P:male genitalia development; IMP:UniProtKB.
DR   GO; GO:0046426; P:negative regulation of JAK-STAT cascade; IMP:UniProtKB.
DR   GO; GO:0006909; P:phagocytosis; IMP:FlyBase.
DR   GO; GO:0046425; P:regulation of JAK-STAT cascade; IMP:FlyBase.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.160.60; -; 2.
DR   Gene3D; 3.30.710.10; -; 1.
DR   InterPro; IPR000210; BTB/POZ-like.
DR   InterPro; IPR011333; BTB/POZ_fold.
DR   InterPro; IPR013069; BTB_POZ.
DR   InterPro; IPR007087; Znf_C2H2.
DR   InterPro; IPR015880; Znf_C2H2-like.
DR   InterPro; IPR013087; Znf_C2H2/integrase_DNA-bd.
DR   Pfam; PF00651; BTB; 1.
DR   SMART; SM00225; BTB; 1.
DR   SMART; SM00355; ZnF_C2H2; 3.
DR   SUPFAM; SSF54695; SSF54695; 1.
DR   PROSITE; PS50097; BTB; 1.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE   2: Evidence at transcript level;
KW   Complete proteome; Developmental protein; DNA-binding; Metal-binding;
KW   Nucleus; Reference proteome; Repeat; Repressor; Transcription;
KW   Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN         1    601       Transcription factor Ken.
FT                                /FTId=PRO_0000046925.
FT   DOMAIN       33    101       BTB. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00037}.
FT   ZN_FING     500    522       C2H2-type 1. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00042}.
FT   ZN_FING     528    551       C2H2-type 2. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00042}.
FT   ZN_FING     567    590       C2H2-type 3. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00042}.
FT   COMPBIAS    245    258       His-rich.
FT   COMPBIAS    257    278       Asn-rich.
SQ   SEQUENCE   601 AA;  67033 MW;  C9AAA437DAD48FE8 CRC64;
     MKEFQRMLML QYSKHGECIL KEIGAAFRGE HPADLTIVCE NKVKLHAHKL VLAAASPLIR
     NLLEDTHLSD CSTTVYFPDV NATYFKFLLD FLYSGQTCIT SRDVNYLHDL LLLLQIKSDS
     WKTTDSAYLS SKCGGLRDRA DRRKQQYTSP QNLEPDQTLK YEVDSVDESR NAADFSSAFN
     SNDNCESAAE CERSGGHNNK EEDEDDCTHK DNKSDKDTDE IVNLSNAPPS GTSGSNSNIS
     TSSNHQQQQH HHHHHHNHNN NNNNNNNNSS SSTINPVNLS LDLRTKSENS ASRTLGSGSD
     HSGIDLAVTA SESTKRKGLF FDSHKDVMKP LSDGSDINSS PENYVVTPHR KRRPGFHNTQ
     SDNQPFTSYP HSLLEELRLA KSTTSPISGF GSEKNMLAHL EDGALNGDTL TPDRKHLLEA
     QRNRAQSPEI PMHLGPQFVY QWQSNQNAAM SAMPNLQSRL SSLSHISLNL DHPEGRSGSA
     SGSGANLAGS NTHASSVREY RCEYCGKQFG MSWNLKTHLR VHTGEKPFAC RLCVAMFKQK
     AHLLKHLCSV HRNVITTTNG ADTENRYSCC FCSMCFESVQ ELVRHLSGHH NNLLLTKNLR
     E
//
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