ID O86931_SALTM Unreviewed; 263 AA.
AC O86931;
DT 01-NOV-1998, integrated into UniProtKB/TrEMBL.
DT 01-NOV-1998, sequence version 1.
DT 24-JAN-2024, entry version 66.
DE RecName: Full=Aminoglycoside (3'') (9) adenylyltransferase {ECO:0000256|ARBA:ARBA00035252, ECO:0000256|PIRNR:PIRNR000819};
DE EC=2.7.7.47 {ECO:0000256|ARBA:ARBA00035126, ECO:0000256|PIRNR:PIRNR000819};
GN Name=aadA1 {ECO:0000313|EMBL:CAA08844.1};
OS Salmonella typhimurium.
OG Plasmid incFI {ECO:0000313|EMBL:CAA08844.1}.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=90371 {ECO:0000313|EMBL:CAA08844.1};
RN [1] {ECO:0000313|EMBL:CAA08844.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Albanian {ECO:0000313|EMBL:CAA08844.1};
RC PLASMID=incFI {ECO:0000313|EMBL:CAA08844.1};
RX PubMed=2827107; DOI=10.1093/nar/15.23.10055;
RA Ouellette M., Roy P.H.;
RT "Homology of ORFs from Tn2603 and from R46 to site-specific recombinases.";
RL Nucleic Acids Res. 15:10055-10055(1987).
RN [2] {ECO:0000313|EMBL:CAA08844.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Albanian {ECO:0000313|EMBL:CAA08844.1};
RC PLASMID=incFI {ECO:0000313|EMBL:CAA08844.1};
RX PubMed=1662753; DOI=10.1111/j.1365-2958.1991.tb00817.x;
RA Hall R.M., Brookes D.E., Stokes H.W.;
RT "Site-specific insertion of genes into integrons: role of the 59-base
RT element and determination of the recombination cross-over point.";
RL Mol. Microbiol. 5:1941-1959(1991).
RN [3] {ECO:0000313|EMBL:CAA08844.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Albanian {ECO:0000313|EMBL:CAA08844.1};
RC PLASMID=incFI {ECO:0000313|EMBL:CAA08844.1};
RX PubMed=9835490;
RA Tosini F., Visca P., Luzzi I., Dionisi A., Pezzella C., Petrucca A.,
RA Carattoli A.;
RT "Class 1 integron-borne multiple-antibiotic resistance carried by IncFI and
RT IncL/M plasmids in Salmonella enterica serotype typhimurium.";
RL Antimicrob. Agents Chemother. 42:3053-3058(1998).
RN [4] {ECO:0000313|EMBL:CAA08844.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Albanian {ECO:0000313|EMBL:CAA08844.1};
RC PLASMID=incFI {ECO:0000313|EMBL:CAA08844.1};
RA Tosini F., Visca P., Luzzi I., Dionisi A.M., Pezzella C., Petrucca A.,
RA Carattoli A.;
RT "Class 1 Integron-borne multiple antibiotic resistance carried by incFI and
RT incL/M plasmids in Salmonella enterica serotype Typhimurium.";
RL Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:CAC81327.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=incFI {ECO:0000313|EMBL:CAC81327.1};
RX PubMed=10477306;
RA Liebert C.A., Hall R.M., Summers A.O.;
RT "Transposon Tn21, flagship of the floating genome.";
RL Microbiol. Mol. Biol. Rev. 63:507-522(1999).
RN [6] {ECO:0000313|EMBL:CAC81327.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=incFI {ECO:0000313|EMBL:CAC81327.1};
RX PubMed=12002644; DOI=10.1089/10766290252913692;
RA Villa L., Visca P., Tosini F., Pezzella C., Carattoli A.;
RT "Composite integron array generated by insertion of an ORF341-type integron
RT within a Tn21-like element.";
RL Microb. Drug Resist. 8:1-8(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + spectinomycin = 9-O-adenylylspectinomycin + diphosphate;
CC Xref=Rhea:RHEA:63228, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:146260, ChEBI:CHEBI:146261;
CC Evidence={ECO:0000256|ARBA:ARBA00001672,
CC ECO:0000256|PIRNR:PIRNR000819};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + streptomycin = 3''-O-adenylylstreptomycin + diphosphate;
CC Xref=Rhea:RHEA:20245, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58007, ChEBI:CHEBI:58605; EC=2.7.7.47;
CC Evidence={ECO:0000256|ARBA:ARBA00035070,
CC ECO:0000256|PIRNR:PIRNR000819};
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DR EMBL; AJ009819; CAA08844.1; -; Genomic_DNA.
DR EMBL; AJ310778; CAC81327.1; -; Genomic_DNA.
DR RefSeq; WP_032489308.1; NG_052227.1.
DR AlphaFoldDB; O86931; -.
DR GO; GO:0070566; F:adenylyltransferase activity; IEA:InterPro.
DR GO; GO:0009012; F:aminoglycoside 3''-adenylyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR CDD; cd05403; NT_KNTase_like; 1.
DR Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR InterPro; IPR024172; AadA/Aad9.
DR InterPro; IPR025184; AadA_C.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR002934; Polymerase_NTP_transf_dom.
DR Pfam; PF13427; AadA_C; 1.
DR Pfam; PF01909; NTP_transf_2; 1.
DR PIRSF; PIRSF000819; Streptomycin_3-adenylyltransf; 1.
DR SUPFAM; SSF81301; Nucleotidyltransferase; 1.
PE 4: Predicted;
KW Antibiotic resistance {ECO:0000256|ARBA:ARBA00023251,
KW ECO:0000256|PIRNR:PIRNR000819};
KW ATP-binding {ECO:0000256|PIRNR:PIRNR000819};
KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR000819};
KW Nucleotidyltransferase {ECO:0000256|PIRNR:PIRNR000819};
KW Plasmid {ECO:0000313|EMBL:CAA08844.1};
KW Transferase {ECO:0000256|PIRNR:PIRNR000819, ECO:0000313|EMBL:CAA08844.1}.
FT DOMAIN 28..76
FT /note="Polymerase nucleotidyl transferase"
FT /evidence="ECO:0000259|Pfam:PF01909"
FT DOMAIN 152..254
FT /note="Adenylyltransferase AadA C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13427"
SQ SEQUENCE 263 AA; 29332 MW; 93CAF9DA7D4038A5 CRC64;
MREAVIAEVS TQLSEVVGVI ERHLEPTLLA VHLYGSAVDG GLKPHSDIDL LVTVTVRLDE
TTRRALINDL LETSASPGES EILRAVEVTI VVHDDIIPWR YPAKRELQFG EWQRNDILAG
IFEPATIDID LAILLTKARE HSVALVGPAA EELFDPVPEQ DLFEALNETL TLWNSPPDWA
GDERNVVLTL SRLWYSAVTG KIAPKDVAAD WAMERLPAQY QPVILEARQA YLGQEEDRLA
SRADQLEEFV HYVKGEITKV VGK
//
