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Database: UniProt
Entry: P00171
LinkDB: P00171
Original site: P00171 
ID   CYB5_BOVIN              Reviewed;         134 AA.
AC   P00171; Q27947; Q28837; Q32PH5;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   19-FEB-2014, entry version 135.
DE   RecName: Full=Cytochrome b5;
GN   Name=CYB5A; Synonyms=CYB5;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=2915932; DOI=10.1093/nar/17.2.799;
RA   Cristiano R.J., Steggles A.W.;
RT   "The complete nucleotide sequence of bovine liver cytochrome b5
RT   mRNA.";
RL   Nucleic Acids Res. 17:799-799(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Liver;
RX   PubMed=8406485; DOI=10.1006/geno.1993.1331;
RA   Cristiano R.J., Giordano S.J., Steggles A.W.;
RT   "The isolation and characterization of the bovine cytochrome b5 gene,
RT   and a transcribed pseudogene.";
RL   Genomics 17:348-354(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   PROTEIN SEQUENCE OF 2-98.
RC   TISSUE=Erythrocyte;
RX   PubMed=4030743;
RA   Abe K., Kimura S., Kizawa R., Anan F.K., Sugita Y.;
RT   "Amino acid sequences of cytochrome b5 from human, porcine, and bovine
RT   erythrocytes and comparison with liver microsomal cytochrome b5.";
RL   J. Biochem. 97:1659-1668(1985).
RN   [5]
RP   PROTEIN SEQUENCE OF 2-11 AND 131-134.
RX   PubMed=4810060; DOI=10.1021/bi00700a005;
RA   Ozols J.;
RT   "Cytochrome b5 from microsomal membranes of equine, bovine, and
RT   porcine livers. Isolation and properties of preparations containing
RT   the membranous segment.";
RL   Biochemistry 13:426-434(1974).
RN   [6]
RP   PROTEIN SEQUENCE OF 2-6 AND 15-18.
RX   PubMed=2752049; DOI=10.1016/0167-4838(89)90143-X;
RA   Ozols J.;
RT   "Structure of cytochrome b5 and its topology in the microsomal
RT   membrane.";
RL   Biochim. Biophys. Acta 997:121-130(1989).
RN   [7]
RP   PROTEIN SEQUENCE OF 6-98.
RX   PubMed=5391285;
RA   Ozols J., Strittmatter P.;
RT   "Correction of the amino acid sequence of calf liver microsomal
RT   cytochrome b5.";
RL   J. Biol. Chem. 244:6617-6618(1969).
RN   [8]
RP   PROTEIN SEQUENCE OF 6-96.
RX   PubMed=5272324; DOI=10.1073/pnas.67.1.442;
RA   Tsugita A., Kobayashi M., Tani S., Kyo S., Rashid M.A., Yoshida Y.,
RA   Kajihara T., Hagihara B.;
RT   "Comparative study of the primary structures of cytochrome b5 from
RT   four species.";
RL   Proc. Natl. Acad. Sci. U.S.A. 67:442-447(1970).
RN   [9]
RP   PROTEIN SEQUENCE OF 92-134.
RX   PubMed=670203;
RA   Fleming P.J., Dailey H.A., Corcoran D., Strittmatter P.;
RT   "The primary structure of the nonpolar segment of bovine cytochrome
RT   b5.";
RL   J. Biol. Chem. 253:5369-5372(1978).
RN   [10]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF OXIDIZED FORM.
RA   Mathews F.S., Argos P., Levine M.;
RT   "The structure of cytochrome b-5 at 2.0-A resolution.";
RL   Cold Spring Harb. Symp. Quant. Biol. 37:387-395(1971).
RN   [11]
RP   X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF REDUCED FORM.
RX   PubMed=1167544;
RA   Argos P., Mathews F.S.;
RT   "The structure of ferrocytochrome b5 at 2.8-A resolution.";
RL   J. Biol. Chem. 250:747-751(1975).
RN   [12]
RP   X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
RX   PubMed=15299727; DOI=10.1107/S0907444995007827;
RA   Durley R.C.E., Mathews F.S.;
RT   "Refinement and structural analysis of bovine cytochrome b5 at 1.5-A
RT   resolution.";
RL   Acta Crystallogr. D 52:65-76(1996).
RN   [13]
RP   X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 8-89, AND MUTAGENESIS.
RX   PubMed=10842340;
RX   DOI=10.1002/(SICI)1097-0134(20000801)40:2<249::AID-PROT70>3.0.CO;2-H;
RA   Wu J., Gan J.-H., Xia Z.-X., Wang Y.-H., Wang W.-H., Xue L.-L.,
RA   Xie Y., Huang Z.-X.;
RT   "Crystal structure of recombinant trypsin-solubilized fragment of
RT   cytochrome b5 and the structural comparison with Val61His mutant.";
RL   Proteins 40:249-257(2000).
RN   [14]
RP   X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 8-89, AND MUTAGENESIS.
RX   PubMed=12136141; DOI=10.1107/S0907444902010016;
RA   Gan J.-H., Wu J., Wang Z.-Q., Wang Y.-H., Huang Z.-X., Xia Z.-X.;
RT   "Structures of V45E and V45Y mutants and structure comparison of a
RT   variety of cytochrome b5 mutants.";
RL   Acta Crystallogr. D 58:1298-1306(2002).
RN   [15]
RP   X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 8-89, CIRCULAR DICHROISM
RP   ANALYSIS, AND MUTAGENESIS.
RX   PubMed=12199707; DOI=10.1046/j.1432-1033.2002.03120.x;
RA   Yao P., Wu J., Wang Y.-H., Sun B.-Y., Xia Z.-X., Huang Z.-X.;
RT   "X-ray crystallography, CD and kinetic studies revealed the essence of
RT   the abnormal behaviors of the cytochrome b5 Phe35->Tyr mutant.";
RL   Eur. J. Biochem. 269:4287-4296(2002).
RN   [16]
RP   STRUCTURE BY NMR.
RX   PubMed=8613986; DOI=10.1006/jmbi.1996.0241;
RA   Muskett F.W., Kelly G.P., Whitford D.;
RT   "The solution structure of bovine ferricytochrome b5 determined using
RT   heteronuclear NMR methods.";
RL   J. Mol. Biol. 258:172-189(1996).
RN   [17]
RP   STRUCTURE BY NMR OF 8-89, AND MUTAGENESIS.
RX   PubMed=11248680; DOI=10.1046/j.1432-1327.2001.02033.x;
RA   Wu Y., Wang Y., Qian C., Lu J., Li E., Wang W., Lu J., Xie Y.,
RA   Wang J., Zhu D., Huang Z., Tang W.;
RT   "Solution structure of cytochrome b5 mutant (E44/48/56A/D60A) and its
RT   interaction with cytochrome c.";
RL   Eur. J. Biochem. 268:1620-1630(2001).
RN   [18]
RP   STRUCTURE BY NMR OF 8-89, AND MUTAGENESIS.
RX   PubMed=11714912; DOI=10.1110/ps.ps.12401;
RA   Qian C., Yao Y., Ye K., Wang J., Tang W., Wang Y., Wang W., Lu J.,
RA   Xie Y., Huang Z.;
RT   "Effects of charged amino-acid mutation on the solution structure of
RT   cytochrome b5 and binding between cytochrome b5 and cytochrome c.";
RL   Protein Sci. 10:2451-2459(2001).
RN   [19]
RP   STRUCTURE BY NMR OF 8-89, AND MUTAGENESIS.
RX   PubMed=12893266; DOI=10.1016/S0006-291X(03)01225-7;
RA   Cao C., Zhang Q., Xue L.-L., Ma J., Wang Y.-H., Wu H., Huang Z.-X.;
RT   "The solution structure of the oxidized bovine microsomal cytochrome
RT   b5 mutant V61H.";
RL   Biochem. Biophys. Res. Commun. 307:600-609(2003).
CC   -!- FUNCTION: Cytochrome b5 is a membrane bound hemoprotein which
CC       function as an electron carrier for several membrane bound
CC       oxygenases.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC       membrane protein; Cytoplasmic side. Microsome membrane; Single-
CC       pass membrane protein; Cytoplasmic side.
CC   -!- SIMILARITY: Belongs to the cytochrome b5 family.
CC   -!- SIMILARITY: Contains 1 cytochrome b5 heme-binding domain.
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DR   EMBL; X13617; CAA31949.1; -; mRNA.
DR   EMBL; M63328; AAC14455.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; M63326; AAC14455.1; JOINED; Genomic_DNA.
DR   EMBL; M63327; AAC14455.1; JOINED; Genomic_DNA.
DR   EMBL; L22966; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC108113; AAI08114.1; -; mRNA.
DR   PIR; A47215; CBBO5.
DR   RefSeq; NP_776458.1; NM_174033.3.
DR   UniGene; Bt.65097; -.
DR   PDB; 1CYO; X-ray; 1.50 A; A=6-98.
DR   PDB; 1EHB; X-ray; 1.90 A; A=8-89.
DR   PDB; 1ES1; X-ray; 2.10 A; A=8-89.
DR   PDB; 1F03; NMR; -; A=8-89.
DR   PDB; 1F04; NMR; -; A=8-89.
DR   PDB; 1HKO; NMR; -; A=2-105.
DR   PDB; 1I5U; NMR; -; A=8-89.
DR   PDB; 1J0Q; NMR; -; A=8-89.
DR   PDB; 1LQX; X-ray; 1.80 A; A=8-89.
DR   PDB; 1LR6; X-ray; 1.90 A; A=8-89.
DR   PDB; 1M20; X-ray; 1.80 A; A=8-89.
DR   PDB; 1M2I; X-ray; 1.80 A; A=8-89.
DR   PDB; 1M2M; X-ray; 1.80 A; A=8-89.
DR   PDB; 1M59; X-ray; 1.90 A; A=8-89.
DR   PDB; 1NX7; NMR; -; A=8-88.
DR   PDB; 1SH4; NMR; -; A=8-88.
DR   PDB; 1U9M; X-ray; 2.00 A; A/B/C/D/E/F=8-88.
DR   PDB; 1U9U; X-ray; 1.86 A; A=8-88.
DR   PDB; 3OZZ; X-ray; 1.70 A; B=8-89.
DR   PDB; 4HIN; X-ray; 2.40 A; A/B/C/D=8-89.
DR   PDBsum; 1CYO; -.
DR   PDBsum; 1EHB; -.
DR   PDBsum; 1ES1; -.
DR   PDBsum; 1F03; -.
DR   PDBsum; 1F04; -.
DR   PDBsum; 1HKO; -.
DR   PDBsum; 1I5U; -.
DR   PDBsum; 1J0Q; -.
DR   PDBsum; 1LQX; -.
DR   PDBsum; 1LR6; -.
DR   PDBsum; 1M20; -.
DR   PDBsum; 1M2I; -.
DR   PDBsum; 1M2M; -.
DR   PDBsum; 1M59; -.
DR   PDBsum; 1NX7; -.
DR   PDBsum; 1SH4; -.
DR   PDBsum; 1U9M; -.
DR   PDBsum; 1U9U; -.
DR   PDBsum; 3OZZ; -.
DR   PDBsum; 4HIN; -.
DR   ProteinModelPortal; P00171; -.
DR   SMR; P00171; 2-105.
DR   PaxDb; P00171; -.
DR   PRIDE; P00171; -.
DR   Ensembl; ENSBTAT00000015944; ENSBTAP00000015944; ENSBTAG00000012012.
DR   GeneID; 281110; -.
DR   KEGG; bta:281110; -.
DR   CTD; 1528; -.
DR   eggNOG; COG5274; -.
DR   GeneTree; ENSGT00510000046507; -.
DR   HOGENOM; HOG000039853; -.
DR   HOVERGEN; HBG002653; -.
DR   InParanoid; P00171; -.
DR   OMA; MAEQSDK; -.
DR   OrthoDB; EOG78H3W2; -.
DR   TreeFam; TF314537; -.
DR   EvolutionaryTrace; P00171; -.
DR   NextBio; 20805181; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005739; C:mitochondrion; IEA:Ensembl.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation-reduction process; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.120.10; -; 1.
DR   InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR   InterPro; IPR018506; Cyt_B5_heme-BS.
DR   Pfam; PF00173; Cyt-b5; 1.
DR   PRINTS; PR00363; CYTOCHROMEB5.
DR   SUPFAM; SSF55856; SSF55856; 1.
DR   PROSITE; PS00191; CYTOCHROME_B5_1; 1.
DR   PROSITE; PS50255; CYTOCHROME_B5_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Acetylation; Complete proteome;
KW   Direct protein sequencing; Electron transport; Endoplasmic reticulum;
KW   Heme; Iron; Membrane; Metal-binding; Microsome; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    134       Cytochrome b5.
FT                                /FTId=PRO_0000166008.
FT   TRANSMEM    109    131       Helical; (Potential).
FT   DOMAIN        9     85       Cytochrome b5 heme-binding.
FT   METAL        44     44       Iron (heme axial ligand).
FT   METAL        68     68       Iron (heme axial ligand).
FT   MOD_RES       2      2       N-acetylalanine.
FT   MOD_RES       7      7       N6-acetyllysine (By similarity).
FT   MOD_RES      10     10       N6-acetyllysine (By similarity).
FT   MOD_RES      19     19       N6-acetyllysine (By similarity).
FT   CONFLICT      2      5       AEES -> ZSZZBA (in Ref. 5; AA sequence).
FT   CONFLICT     16     18       EIQ -> QIE (in Ref. 7; AA sequence).
FT   CONFLICT     18     18       Q -> E (in Ref. 8; AA sequence).
FT   CONFLICT     62     62       N -> D (in Ref. 7; AA sequence and 8; AA
FT                                sequence).
FT   CONFLICT    134    134       N -> D (in Ref. 5; AA sequence).
FT   STRAND        9     12
FT   HELIX        14     17
FT   STRAND       21     24
FT   STRAND       25     30
FT   STRAND       33     36
FT   TURN         38     43
FT   HELIX        49     54
FT   STRAND       56     58
FT   HELIX        60     65
FT   HELIX        70     76
FT   TURN         77     79
FT   STRAND       80     84
FT   HELIX        86     91
SQ   SEQUENCE   134 AA;  15329 MW;  7B7B605158D97525 CRC64;
     MAEESSKAVK YYTLEEIQKH NNSKSTWLIL HYKVYDLTKF LEEHPGGEEV LREQAGGDAT
     ENFEDVGHST DARELSKTFI IGELHPDDRS KITKPSESII TTIDSNPSWW TNWLIPAISA
     LFVALIYHLY TSEN
//
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