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Database: UniProt
Entry: P02547
LinkDB: P02547
Original site: P02547 
ID   NFL_PIG                 Reviewed;         549 AA.
AC   P02547;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2007, sequence version 3.
DT   29-OCT-2014, entry version 94.
DE   RecName: Full=Neurofilament light polypeptide;
DE            Short=NF-L;
DE   AltName: Full=68 kDa neurofilament protein;
DE   AltName: Full=Neurofilament triplet L protein;
GN   Name=NEFL;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae;
OC   Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   PROTEIN SEQUENCE OF 2-549.
RX   PubMed=3920075; DOI=10.1016/0014-5793(85)80357-4;
RA   Geisler N., Plessmann U., Weber K.;
RT   "The complete amino acid sequence of the major mammalian neurofilament
RT   protein (NF-L).";
RL   FEBS Lett. 182:475-478(1985).
RN   [2]
RP   PROTEIN SEQUENCE OF 2-83 AND 279-549.
RX   PubMed=10872323;
RA   Geisler N., Kaufmann E., Fischer S., Plessmann U., Weber K.;
RT   "Neurofilament architecture combines structural principles of
RT   intermediate filaments with carboxy-terminal extensions increasing in
RT   size between triplet proteins.";
RL   EMBO J. 2:1295-1302(1983).
CC   -!- FUNCTION: Neurofilaments usually contain three intermediate
CC       filament proteins: L, M, and H which are involved in the
CC       maintenance of neuronal caliber.
CC   -!- SUBUNIT: Interacts with ARHGEF28. Interacts with TRIM2.
CC       {ECO:0000250}.
CC   -!- DOMAIN: The extra mass and high charge density that distinguish
CC       the neurofilament proteins from all other intermediate filament
CC       proteins are due to the tailpiece extensions. This region may form
CC       a charged scaffolding structure suitable for interaction with
CC       other neuronal components or ions.
CC   -!- PTM: O-glycosylated. {ECO:0000250}.
CC   -!- PTM: Phosphorylated in the head and rod regions by the PKC kinase
CC       PKN1, leading to the inhibition of polymerization. {ECO:0000250}.
CC   -!- PTM: Ubiquitinated in the presence of TRIM2 and UBE2D1.
CC       {ECO:0000250}.
CC   -!- MISCELLANEOUS: NF-L is the most abundant of the three
CC       neurofilament proteins and, like the other nonepithelial
CC       intermediate filament proteins, it can form homopolymeric 10-nm
CC       filaments.
CC   -!- SIMILARITY: Belongs to the intermediate filament family.
CC       {ECO:0000305}.
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DR   PIR; A91337; QFPGL.
DR   DisProt; DP00151; -.
DR   ProteinModelPortal; P02547; -.
DR   SMR; P02547; 87-123, 323-395.
DR   STRING; 9823.ENSSSCP00000010302; -.
DR   PaxDb; P02547; -.
DR   eggNOG; NOG145720; -.
DR   HOGENOM; HOG000230977; -.
DR   HOVERGEN; HBG013015; -.
DR   InParanoid; P02547; -.
DR   GO; GO:0030424; C:axon; ISS:UniProtKB.
DR   GO; GO:0005883; C:neurofilament; ISS:UniProtKB.
DR   GO; GO:0042802; F:identical protein binding; ISS:UniProtKB.
DR   GO; GO:0008022; F:protein C-terminus binding; ISS:UniProtKB.
DR   GO; GO:0005200; F:structural constituent of cytoskeleton; ISS:UniProtKB.
DR   GO; GO:0008089; P:anterograde axon cargo transport; ISS:UniProtKB.
DR   GO; GO:0019896; P:axon transport of mitochondrion; ISS:UniProtKB.
DR   GO; GO:0045109; P:intermediate filament organization; ISS:UniProtKB.
DR   GO; GO:0033693; P:neurofilament bundle assembly; ISS:UniProtKB.
DR   GO; GO:0008090; P:retrograde axon cargo transport; ISS:UniProtKB.
DR   InterPro; IPR001664; IF.
DR   InterPro; IPR006821; Intermed_filament_DNA-bd.
DR   InterPro; IPR018039; Intermediate_filament_CS.
DR   InterPro; IPR027692; NF-L.
DR   PANTHER; PTHR23239; PTHR23239; 1.
DR   PANTHER; PTHR23239:SF22; PTHR23239:SF22; 1.
DR   Pfam; PF00038; Filament; 1.
DR   Pfam; PF04732; Filament_head; 1.
DR   PROSITE; PS00226; IF; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Coiled coil; Complete proteome;
KW   Direct protein sequencing; Glycoprotein; Intermediate filament;
KW   Phosphoprotein; Reference proteome; Ubl conjugation.
FT   INIT_MET      1      1       Removed. {ECO:0000269|PubMed:10872323,
FT                                ECO:0000269|PubMed:3920075}.
FT   CHAIN         2    549       Neurofilament light polypeptide.
FT                                /FTId=PRO_0000063789.
FT   REGION        2     92       Head.
FT   REGION       93    396       Rod.
FT   REGION       93    124       Coil 1A.
FT   REGION      125    137       Linker 1.
FT   REGION      138    233       Coil 1B.
FT   REGION      234    252       Linker 12.
FT   REGION      253    271       Coil 2A.
FT   REGION      272    280       Linker 2.
FT   REGION      281    396       Coil 2B.
FT   REGION      381    391       Epitope; recognized by IF-specific
FT                                monoclonal antibody.
FT   REGION      397    549       Tail.
FT   REGION      397    443       Tail, subdomain A.
FT   REGION      444    549       Tail, subdomain B (acidic).
FT   MOD_RES       2      2       N-acetylserine. {ECO:0000250}.
FT   MOD_RES      43     43       Phosphotyrosine. {ECO:0000250}.
FT   MOD_RES      56     56       Phosphoserine. {ECO:0000250}.
FT   MOD_RES      66     66       Phosphoserine. {ECO:0000250}.
FT   MOD_RES     472    472       Phosphoserine. {ECO:0000250}.
FT   CARBOHYD     21     21       O-linked (GlcNAc). {ECO:0000250}.
FT   CARBOHYD     27     27       O-linked (GlcNAc). {ECO:0000250}.
FT   UNSURE      323    323       R or K.
SQ   SEQUENCE   549 AA;  62072 MW;  23CD481372F7024B CRC64;
     MSSFYSEPYY STSYKRRYVE TPRVHISSVR SGYSTARSAY SSYSAPVSSS LSVRRSYSSS
     SGLMPSLENL DLSQVAAISN DLKSIRTQEK AQLQDLNDRF ASFIERVHEL EQQNKVLEAQ
     LLVLRQKHSE PSRFRALYEQ EIRDLRLAAE DATNEKQALQ GEREGLEETL RNLQARYEEE
     VLSREDAEGR LMEARKGADE AALARAELEK RIDSLMDEIA FLKKVHEEEI AELQAQIQYA
     QISVEMDVSS KPDLSAALKD IRAQYEKLAA KNMQNAEEWF KSRFTVLTES AAKNTDAVRA
     AKDEVSESRR LLKAKTLEIE ACRGMNEALE KQLQELEDKQ NADISAMQDT INKLENELRT
     TKSEMARYLK EYQDLLNVKM ALDIEIAAYR KLLEGEETRL SFTSVGSLTT GYSQSSQVFG
     RSAYGGLQTS SYLMSTRSFP SYYTSHVQEE QIEVEETIEA AKAEEAKDEP PSEGEAEEEG
     KEKEEAEAEA EAEEEGAQEE EEAAEKEESE EAKEEEGGEG EQGEETKEAE EEEKKDEGAG
     EEQATKKKD
//
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