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Database: UniProt
Entry: P03018
LinkDB: P03018
Original site: P03018 
ID   UVRD_ECOLI              Reviewed;         720 AA.
AC   P03018; P76758; Q2M8B9; Q47709;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   26-NOV-2014, entry version 144.
DE   RecName: Full=DNA helicase II;
DE            EC=3.6.4.12;
GN   Name=uvrD; Synonyms=mutU, pdeB, rad, recL;
GN   OrderedLocusNames=b3813, JW3786;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6379604; DOI=10.1093/nar/12.14.5789;
RA   Finch P.W., Emmerson P.T.;
RT   "The nucleotide sequence of the uvrD gene of E. coli.";
RL   Nucleic Acids Res. 12:5789-5799(1984).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX   PubMed=2943729;
RA   Yamamoto Y., Ogawa T., Shinagawa H., Nakayama T., Matsuo H., Ogawa H.;
RT   "Determination of the initiation sites of transcription and
RT   translation of the uvrD gene of Escherichia coli.";
RL   J. Biochem. 99:1579-1590(1986).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=1379743; DOI=10.1126/science.1379743;
RA   Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT   "Analysis of the Escherichia coli genome: DNA sequence of the region
RT   from 84.5 to 86.5 minutes.";
RL   Science 257:771-778(1992).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-258.
RX   PubMed=6324092; DOI=10.1093/nar/11.24.8625;
RA   Easton A.M., Kushner S.R.;
RT   "Transcription of the uvrD gene of Escherichia coli is controlled by
RT   the lexA repressor and by attenuation.";
RL   Nucleic Acids Res. 11:8625-8640(1983).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17.
RC   STRAIN=K12;
RX   PubMed=2254268;
RA   Colloms S.D., Sykora P., Szatmari G., Sherratt D.J.;
RT   "Recombination at ColE1 cer requires the Escherichia coli xerC gene
RT   product, a member of the lambda integrase family of site-specific
RT   recombinases.";
RL   J. Bacteriol. 172:6973-6980(1990).
RN   [8]
RP   VARIANT UVRD252.
RX   PubMed=8419285;
RA   Washburn B.K., Kushner S.R.;
RT   "Characterization of DNA helicase II from a uvrD252 mutant of
RT   Escherichia coli.";
RL   J. Bacteriol. 175:341-350(1993).
CC   -!- FUNCTION: Has both ATPase and helicase activities. Unwinds DNA
CC       duplexes with 3' to 5' polarity with respect to the bound strand
CC       and initiates unwinding most effectively when a single-stranded
CC       region is present. Involved in the post-incision events of
CC       nucleotide excision repair and methyl-directed mismatch repair.
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC   -!- INTERACTION:
CC       P0A9P0:lpdA; NbExp=2; IntAct=EBI-559573, EBI-542856;
CC       P25665:metE; NbExp=2; IntAct=EBI-559573, EBI-551247;
CC       P23367:mutL; NbExp=7; IntAct=EBI-559573, EBI-554913;
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Contains 1 uvrD-like helicase ATP-binding domain.
CC       {ECO:0000255|PROSITE-ProRule:PRU00560}.
CC   -!- SIMILARITY: Contains 1 uvrD-like helicase C-terminal domain.
CC       {ECO:0000255|PROSITE-ProRule:PRU00617}.
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DR   EMBL; X00738; CAA25321.1; -; Genomic_DNA.
DR   EMBL; D00069; BAA00048.1; -; Genomic_DNA.
DR   EMBL; X04037; CAA27671.1; -; Genomic_DNA.
DR   EMBL; M87049; AAA67609.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76816.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77487.1; -; Genomic_DNA.
DR   EMBL; X00225; CAA25043.1; -; Genomic_DNA.
DR   EMBL; M38257; AAA24765.1; -; Genomic_DNA.
DR   PIR; F65185; HJECD2.
DR   RefSeq; NP_418258.1; NC_000913.3.
DR   RefSeq; YP_491628.1; NC_007779.1.
DR   PDB; 2IS1; X-ray; 2.90 A; A/B=1-680.
DR   PDB; 2IS2; X-ray; 3.00 A; A/B=1-680.
DR   PDB; 2IS4; X-ray; 2.60 A; A/B=1-680.
DR   PDB; 2IS6; X-ray; 2.20 A; A/B=1-680.
DR   PDB; 3LFU; X-ray; 1.80 A; A=1-647.
DR   PDBsum; 2IS1; -.
DR   PDBsum; 2IS2; -.
DR   PDBsum; 2IS4; -.
DR   PDBsum; 2IS6; -.
DR   PDBsum; 3LFU; -.
DR   DisProt; DP00684; -.
DR   ProteinModelPortal; P03018; -.
DR   SMR; P03018; 1-656.
DR   DIP; DIP-11103N; -.
DR   IntAct; P03018; 37.
DR   MINT; MINT-1223204; -.
DR   STRING; 511145.b3813; -.
DR   PaxDb; P03018; -.
DR   PRIDE; P03018; -.
DR   EnsemblBacteria; AAC76816; AAC76816; b3813.
DR   EnsemblBacteria; BAE77487; BAE77487; BAE77487.
DR   GeneID; 12934346; -.
DR   GeneID; 948347; -.
DR   KEGG; ecj:Y75_p3364; -.
DR   KEGG; eco:b3813; -.
DR   PATRIC; 32123127; VBIEscCol129921_3929.
DR   EchoBASE; EB1057; -.
DR   EcoGene; EG11064; uvrD.
DR   eggNOG; COG0210; -.
DR   HOGENOM; HOG000033016; -.
DR   InParanoid; P03018; -.
DR   KO; K03657; -.
DR   OMA; RLQIAFQ; -.
DR   OrthoDB; EOG64N9TW; -.
DR   PhylomeDB; P03018; -.
DR   BioCyc; EcoCyc:EG11064-MONOMER; -.
DR   BioCyc; ECOL316407:JW3786-MONOMER; -.
DR   BioCyc; MetaCyc:EG11064-MONOMER; -.
DR   EvolutionaryTrace; P03018; -.
DR   PRO; PR:P03018; -.
DR   Genevestigator; P03018; -.
DR   GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IDA:CACAO.
DR   GO; GO:0015616; F:DNA translocase activity; IDA:EcoCyc.
DR   GO; GO:0017116; F:single-stranded DNA-dependent ATP-dependent DNA helicase activity; IDA:EcoCyc.
DR   GO; GO:0006200; P:ATP catabolic process; IDA:GOC.
DR   GO; GO:0032508; P:DNA duplex unwinding; IDA:EcoCyc.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IMP:EcoCyc.
DR   GO; GO:0006289; P:nucleotide-excision repair; IDA:EcoCyc.
DR   GO; GO:0051260; P:protein homooligomerization; IDA:EcoCyc.
DR   GO; GO:0031297; P:replication fork processing; IMP:EcoCyc.
DR   GO; GO:0070581; P:rolling circle DNA replication; IMP:EcoCyc.
DR   GO; GO:0009432; P:SOS response; IGI:EcoCyc.
DR   Gene3D; 1.10.10.160; -; 1.
DR   Gene3D; 3.40.50.300; -; 4.
DR   InterPro; IPR013986; DExx_box_DNA_helicase_dom.
DR   InterPro; IPR005753; DNA_helicase_ATP-dep_UvrD.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01075; uvrD; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Complete proteome;
KW   Direct protein sequencing; DNA damage; DNA repair; DNA replication;
KW   DNA-binding; Helicase; Hydrolase; Nucleotide-binding;
KW   Reference proteome; SOS response.
FT   CHAIN         1    720       DNA helicase II.
FT                                /FTId=PRO_0000102072.
FT   DOMAIN        8    286       UvrD-like helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00560}.
FT   DOMAIN      287    564       UvrD-like helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00617}.
FT   NP_BIND      32     37       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00560}.
FT   BINDING     284    284       ATP. {ECO:0000250}.
FT   VARIANT      30     30       G -> D (in uvrD252, which is UV
FT                                sensitive).
FT   CONFLICT    224    224       D -> N (in Ref. 1; CAA25321).
FT                                {ECO:0000305}.
FT   CONFLICT    291    293       SAA -> NAR (in Ref. 1; CAA25321).
FT                                {ECO:0000305}.
FT   CONFLICT    330    330       A -> T (in Ref. 1; CAA25321).
FT                                {ECO:0000305}.
FT   CONFLICT    540    540       L -> V (in Ref. 1; CAA25321).
FT                                {ECO:0000305}.
FT   HELIX         4      7       {ECO:0000244|PDB:3LFU}.
FT   STRAND        8     10       {ECO:0000244|PDB:2IS1}.
FT   HELIX        12     18       {ECO:0000244|PDB:3LFU}.
FT   STRAND       25     29       {ECO:0000244|PDB:3LFU}.
FT   STRAND       31     33       {ECO:0000244|PDB:2IS2}.
FT   HELIX        35     48       {ECO:0000244|PDB:3LFU}.
FT   HELIX        54     56       {ECO:0000244|PDB:3LFU}.
FT   STRAND       57     63       {ECO:0000244|PDB:3LFU}.
FT   HELIX        64     78       {ECO:0000244|PDB:3LFU}.
FT   STRAND       86     89       {ECO:0000244|PDB:3LFU}.
FT   HELIX        90    100       {ECO:0000244|PDB:3LFU}.
FT   HELIX       103    105       {ECO:0000244|PDB:3LFU}.
FT   STRAND      112    114       {ECO:0000244|PDB:3LFU}.
FT   HELIX       116    129       {ECO:0000244|PDB:3LFU}.
FT   TURN        134    136       {ECO:0000244|PDB:3LFU}.
FT   HELIX       139    151       {ECO:0000244|PDB:3LFU}.
FT   TURN        156    158       {ECO:0000244|PDB:2IS6}.
FT   HELIX       166    184       {ECO:0000244|PDB:3LFU}.
FT   STRAND      186    188       {ECO:0000244|PDB:3LFU}.
FT   HELIX       189    202       {ECO:0000244|PDB:3LFU}.
FT   HELIX       204    213       {ECO:0000244|PDB:3LFU}.
FT   STRAND      216    221       {ECO:0000244|PDB:3LFU}.
FT   HELIX       222    224       {ECO:0000244|PDB:3LFU}.
FT   HELIX       227    237       {ECO:0000244|PDB:3LFU}.
FT   TURN        238    240       {ECO:0000244|PDB:3LFU}.
FT   STRAND      242    247       {ECO:0000244|PDB:3LFU}.
FT   HELIX       249    251       {ECO:0000244|PDB:3LFU}.
FT   HELIX       255    257       {ECO:0000244|PDB:3LFU}.
FT   HELIX       263    270       {ECO:0000244|PDB:3LFU}.
FT   STRAND      275    279       {ECO:0000244|PDB:3LFU}.
FT   STRAND      283    285       {ECO:0000244|PDB:3LFU}.
FT   HELIX       287    297       {ECO:0000244|PDB:3LFU}.
FT   STRAND      300    302       {ECO:0000244|PDB:2IS6}.
FT   STRAND      318    325       {ECO:0000244|PDB:3LFU}.
FT   HELIX       326    342       {ECO:0000244|PDB:3LFU}.
FT   HELIX       347    349       {ECO:0000244|PDB:3LFU}.
FT   STRAND      350    356       {ECO:0000244|PDB:3LFU}.
FT   HELIX       357    359       {ECO:0000244|PDB:3LFU}.
FT   HELIX       360    369       {ECO:0000244|PDB:3LFU}.
FT   STRAND      374    379       {ECO:0000244|PDB:3LFU}.
FT   HELIX       382    384       {ECO:0000244|PDB:3LFU}.
FT   HELIX       386    399       {ECO:0000244|PDB:3LFU}.
FT   HELIX       404    410       {ECO:0000244|PDB:3LFU}.
FT   HELIX       420    432       {ECO:0000244|PDB:3LFU}.
FT   HELIX       437    446       {ECO:0000244|PDB:3LFU}.
FT   HELIX       452    471       {ECO:0000244|PDB:3LFU}.
FT   TURN        472    474       {ECO:0000244|PDB:3LFU}.
FT   HELIX       477    487       {ECO:0000244|PDB:3LFU}.
FT   HELIX       490    495       {ECO:0000244|PDB:3LFU}.
FT   HELIX       500    519       {ECO:0000244|PDB:3LFU}.
FT   HELIX       530    540       {ECO:0000244|PDB:3LFU}.
FT   HELIX       542    544       {ECO:0000244|PDB:2IS6}.
FT   STRAND      554    558       {ECO:0000244|PDB:3LFU}.
FT   HELIX       560    562       {ECO:0000244|PDB:3LFU}.
FT   STRAND      567    572       {ECO:0000244|PDB:3LFU}.
FT   TURN        579    581       {ECO:0000244|PDB:3LFU}.
FT   HELIX       583    586       {ECO:0000244|PDB:2IS6}.
FT   STRAND      587    590       {ECO:0000244|PDB:2IS6}.
FT   HELIX       592    603       {ECO:0000244|PDB:3LFU}.
FT   STRAND      606    620       {ECO:0000244|PDB:3LFU}.
FT   STRAND      623    626       {ECO:0000244|PDB:3LFU}.
FT   HELIX       631    635       {ECO:0000244|PDB:3LFU}.
FT   HELIX       638    640       {ECO:0000244|PDB:3LFU}.
FT   STRAND      641    643       {ECO:0000244|PDB:3LFU}.
FT   STRAND      646    649       {ECO:0000244|PDB:2IS4}.
SQ   SEQUENCE   720 AA;  81990 MW;  FA68E7267C77B49A CRC64;
     MDVSYLLDSL NDKQREAVAA PRSNLLVLAG AGSGKTRVLV HRIAWLMSVE NCSPYSIMAV
     TFTNKAAAEM RHRIGQLMGT SQGGMWVGTF HGLAHRLLRA HHMDANLPQD FQILDSEDQL
     RLLKRLIKAM NLDEKQWPPR QAMWYINSQK DEGLRPHHIQ SYGNPVEQTW QKVYQAYQEA
     CDRAGLVDFA ELLLRAHELW LNKPHILQHY RERFTNILVD EFQDTNNIQY AWIRLLAGDT
     GKVMIVGDDD QSIYGWRGAQ VENIQRFLND FPGAETIRLE QNYRSTSNIL SAANALIENN
     NGRLGKKLWT DGADGEPISL YCAFNELDEA RFVVNRIKTW QDNGGALAEC AILYRSNAQS
     RVLEEALLQA SMPYRIYGGM RFFERQEIKD ALSYLRLIAN RNDDAAFERV VNTPTRGIGD
     RTLDVVRQTS RDRQLTLWQA CRELLQEKAL AGRAASALQR FMELIDALAQ ETADMPLHVQ
     TDRVIKDSGL RTMYEQEKGE KGQTRIENLE ELVTATRQFS YNEEDEDLMP LQAFLSHAAL
     EAGEGQADTW QDAVQLMTLH SAKGLEFPQV FIVGMEEGMF PSQMSLDEGG RLEEERRLAY
     VGVTRAMQKL TLTYAETRRL YGKEVYHRPS RFIGELPEEC VEEVRLRATV SRPVSHQRMG
     TPMVENDSGY KLGQRVRHAK FGEGTIVNME GSGEHSRLQV AFQGQGIKWL VAAYARLESV
//
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