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Database: UniProt
Entry: P03018
LinkDB: P03018
Original site: P03018 
ID   UVRD_ECOLI              Reviewed;         720 AA.
AC   P03018; P76758; Q2M8B9; Q47709;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   01-OCT-2014, entry version 142.
DE   RecName: Full=DNA helicase II;
DE            EC=3.6.4.12;
GN   Name=uvrD; Synonyms=mutU, pdeB, rad, recL;
GN   OrderedLocusNames=b3813, JW3786;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6379604; DOI=10.1093/nar/12.14.5789;
RA   Finch P.W., Emmerson P.T.;
RT   "The nucleotide sequence of the uvrD gene of E. coli.";
RL   Nucleic Acids Res. 12:5789-5799(1984).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX   PubMed=2943729;
RA   Yamamoto Y., Ogawa T., Shinagawa H., Nakayama T., Matsuo H., Ogawa H.;
RT   "Determination of the initiation sites of transcription and
RT   translation of the uvrD gene of Escherichia coli.";
RL   J. Biochem. 99:1579-1590(1986).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=1379743; DOI=10.1126/science.1379743;
RA   Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT   "Analysis of the Escherichia coli genome: DNA sequence of the region
RT   from 84.5 to 86.5 minutes.";
RL   Science 257:771-778(1992).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-258.
RX   PubMed=6324092; DOI=10.1093/nar/11.24.8625;
RA   Easton A.M., Kushner S.R.;
RT   "Transcription of the uvrD gene of Escherichia coli is controlled by
RT   the lexA repressor and by attenuation.";
RL   Nucleic Acids Res. 11:8625-8640(1983).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17.
RC   STRAIN=K12;
RX   PubMed=2254268;
RA   Colloms S.D., Sykora P., Szatmari G., Sherratt D.J.;
RT   "Recombination at ColE1 cer requires the Escherichia coli xerC gene
RT   product, a member of the lambda integrase family of site-specific
RT   recombinases.";
RL   J. Bacteriol. 172:6973-6980(1990).
RN   [8]
RP   VARIANT UVRD252.
RX   PubMed=8419285;
RA   Washburn B.K., Kushner S.R.;
RT   "Characterization of DNA helicase II from a uvrD252 mutant of
RT   Escherichia coli.";
RL   J. Bacteriol. 175:341-350(1993).
CC   -!- FUNCTION: Has both ATPase and helicase activities. Unwinds DNA
CC       duplexes with 3' to 5' polarity with respect to the bound strand
CC       and initiates unwinding most effectively when a single-stranded
CC       region is present. Involved in the post-incision events of
CC       nucleotide excision repair and methyl-directed mismatch repair.
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC   -!- INTERACTION:
CC       P0A9P0:lpdA; NbExp=2; IntAct=EBI-559573, EBI-542856;
CC       P25665:metE; NbExp=2; IntAct=EBI-559573, EBI-551247;
CC       P23367:mutL; NbExp=7; IntAct=EBI-559573, EBI-554913;
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Contains 1 uvrD-like helicase ATP-binding domain.
CC       {ECO:0000255|PROSITE-ProRule:PRU00560}.
CC   -!- SIMILARITY: Contains 1 uvrD-like helicase C-terminal domain.
CC       {ECO:0000255|PROSITE-ProRule:PRU00617}.
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DR   EMBL; X00738; CAA25321.1; -; Genomic_DNA.
DR   EMBL; D00069; BAA00048.1; -; Genomic_DNA.
DR   EMBL; X04037; CAA27671.1; -; Genomic_DNA.
DR   EMBL; M87049; AAA67609.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76816.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77487.1; -; Genomic_DNA.
DR   EMBL; X00225; CAA25043.1; -; Genomic_DNA.
DR   EMBL; M38257; AAA24765.1; -; Genomic_DNA.
DR   PIR; F65185; HJECD2.
DR   RefSeq; NP_418258.1; NC_000913.3.
DR   RefSeq; YP_491628.1; NC_007779.1.
DR   PDB; 2IS1; X-ray; 2.90 A; A/B=1-680.
DR   PDB; 2IS2; X-ray; 3.00 A; A/B=1-680.
DR   PDB; 2IS4; X-ray; 2.60 A; A/B=1-680.
DR   PDB; 2IS6; X-ray; 2.20 A; A/B=1-680.
DR   PDB; 3LFU; X-ray; 1.80 A; A=1-647.
DR   PDBsum; 2IS1; -.
DR   PDBsum; 2IS2; -.
DR   PDBsum; 2IS4; -.
DR   PDBsum; 2IS6; -.
DR   PDBsum; 3LFU; -.
DR   DisProt; DP00684; -.
DR   ProteinModelPortal; P03018; -.
DR   SMR; P03018; 1-656.
DR   DIP; DIP-11103N; -.
DR   IntAct; P03018; 37.
DR   MINT; MINT-1223204; -.
DR   STRING; 511145.b3813; -.
DR   PaxDb; P03018; -.
DR   PRIDE; P03018; -.
DR   EnsemblBacteria; AAC76816; AAC76816; b3813.
DR   EnsemblBacteria; BAE77487; BAE77487; BAE77487.
DR   GeneID; 12934346; -.
DR   GeneID; 948347; -.
DR   KEGG; ecj:Y75_p3364; -.
DR   KEGG; eco:b3813; -.
DR   PATRIC; 32123127; VBIEscCol129921_3929.
DR   EchoBASE; EB1057; -.
DR   EcoGene; EG11064; uvrD.
DR   eggNOG; COG0210; -.
DR   HOGENOM; HOG000033016; -.
DR   KO; K03657; -.
DR   OMA; RLQIAFQ; -.
DR   OrthoDB; EOG64N9TW; -.
DR   PhylomeDB; P03018; -.
DR   BioCyc; EcoCyc:EG11064-MONOMER; -.
DR   BioCyc; ECOL316407:JW3786-MONOMER; -.
DR   BioCyc; MetaCyc:EG11064-MONOMER; -.
DR   EvolutionaryTrace; P03018; -.
DR   PRO; PR:P03018; -.
DR   Genevestigator; P03018; -.
DR   GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IDA:CACAO.
DR   GO; GO:0015616; F:DNA translocase activity; IDA:EcoCyc.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0017116; F:single-stranded DNA-dependent ATP-dependent DNA helicase activity; IDA:EcoCyc.
DR   GO; GO:0006200; P:ATP catabolic process; IDA:GOC.
DR   GO; GO:0032508; P:DNA duplex unwinding; IDA:EcoCyc.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IMP:EcoCyc.
DR   GO; GO:0006289; P:nucleotide-excision repair; IDA:EcoCyc.
DR   GO; GO:0051260; P:protein homooligomerization; IDA:EcoCyc.
DR   GO; GO:0031297; P:replication fork processing; IMP:EcoCyc.
DR   GO; GO:0070581; P:rolling circle DNA replication; IMP:EcoCyc.
DR   GO; GO:0009432; P:SOS response; IGI:EcoCyc.
DR   Gene3D; 1.10.10.160; -; 1.
DR   Gene3D; 3.40.50.300; -; 4.
DR   InterPro; IPR013986; DExx_box_DNA_helicase_dom.
DR   InterPro; IPR005753; DNA_helicase_ATP-dep_UvrD.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01075; uvrD; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Complete proteome;
KW   Direct protein sequencing; DNA damage; DNA repair; DNA replication;
KW   DNA-binding; Helicase; Hydrolase; Nucleotide-binding;
KW   Reference proteome; SOS response.
FT   CHAIN         1    720       DNA helicase II.
FT                                /FTId=PRO_0000102072.
FT   DOMAIN        8    286       UvrD-like helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00560}.
FT   DOMAIN      287    564       UvrD-like helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00617}.
FT   NP_BIND      32     37       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00560}.
FT   BINDING     284    284       ATP. {ECO:0000250}.
FT   VARIANT      30     30       G -> D (in uvrD252, which is UV
FT                                sensitive).
FT   CONFLICT    224    224       D -> N (in Ref. 1; CAA25321).
FT                                {ECO:0000305}.
FT   CONFLICT    291    293       SAA -> NAR (in Ref. 1; CAA25321).
FT                                {ECO:0000305}.
FT   CONFLICT    330    330       A -> T (in Ref. 1; CAA25321).
FT                                {ECO:0000305}.
FT   CONFLICT    540    540       L -> V (in Ref. 1; CAA25321).
FT                                {ECO:0000305}.
FT   HELIX         4      7
FT   STRAND        8     10
FT   HELIX        12     18
FT   STRAND       25     29
FT   STRAND       31     33
FT   HELIX        35     48
FT   HELIX        54     56
FT   STRAND       57     63
FT   HELIX        64     78
FT   STRAND       86     89
FT   HELIX        90    100
FT   HELIX       103    105
FT   STRAND      112    114
FT   HELIX       116    129
FT   TURN        134    136
FT   HELIX       139    151
FT   TURN        156    158
FT   HELIX       166    184
FT   STRAND      186    188
FT   HELIX       189    202
FT   HELIX       204    213
FT   STRAND      216    221
FT   HELIX       222    224
FT   HELIX       227    237
FT   TURN        238    240
FT   STRAND      242    247
FT   HELIX       249    251
FT   HELIX       255    257
FT   HELIX       263    270
FT   STRAND      275    279
FT   STRAND      283    285
FT   HELIX       287    297
FT   STRAND      300    302
FT   STRAND      318    325
FT   HELIX       326    342
FT   HELIX       347    349
FT   STRAND      350    356
FT   HELIX       357    359
FT   HELIX       360    369
FT   STRAND      374    379
FT   HELIX       382    384
FT   HELIX       386    399
FT   HELIX       404    410
FT   HELIX       420    432
FT   HELIX       437    446
FT   HELIX       452    471
FT   TURN        472    474
FT   HELIX       477    487
FT   HELIX       490    495
FT   HELIX       500    519
FT   HELIX       530    540
FT   HELIX       542    544
FT   STRAND      554    558
FT   HELIX       560    562
FT   STRAND      567    572
FT   TURN        579    581
FT   HELIX       583    586
FT   STRAND      587    590
FT   HELIX       592    603
FT   STRAND      606    620
FT   STRAND      623    626
FT   HELIX       631    635
FT   HELIX       638    640
FT   STRAND      641    643
FT   STRAND      646    649
SQ   SEQUENCE   720 AA;  81990 MW;  FA68E7267C77B49A CRC64;
     MDVSYLLDSL NDKQREAVAA PRSNLLVLAG AGSGKTRVLV HRIAWLMSVE NCSPYSIMAV
     TFTNKAAAEM RHRIGQLMGT SQGGMWVGTF HGLAHRLLRA HHMDANLPQD FQILDSEDQL
     RLLKRLIKAM NLDEKQWPPR QAMWYINSQK DEGLRPHHIQ SYGNPVEQTW QKVYQAYQEA
     CDRAGLVDFA ELLLRAHELW LNKPHILQHY RERFTNILVD EFQDTNNIQY AWIRLLAGDT
     GKVMIVGDDD QSIYGWRGAQ VENIQRFLND FPGAETIRLE QNYRSTSNIL SAANALIENN
     NGRLGKKLWT DGADGEPISL YCAFNELDEA RFVVNRIKTW QDNGGALAEC AILYRSNAQS
     RVLEEALLQA SMPYRIYGGM RFFERQEIKD ALSYLRLIAN RNDDAAFERV VNTPTRGIGD
     RTLDVVRQTS RDRQLTLWQA CRELLQEKAL AGRAASALQR FMELIDALAQ ETADMPLHVQ
     TDRVIKDSGL RTMYEQEKGE KGQTRIENLE ELVTATRQFS YNEEDEDLMP LQAFLSHAAL
     EAGEGQADTW QDAVQLMTLH SAKGLEFPQV FIVGMEEGMF PSQMSLDEGG RLEEERRLAY
     VGVTRAMQKL TLTYAETRRL YGKEVYHRPS RFIGELPEEC VEEVRLRATV SRPVSHQRMG
     TPMVENDSGY KLGQRVRHAK FGEGTIVNME GSGEHSRLQV AFQGQGIKWL VAAYARLESV
//
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