ID OPS1_DROME Reviewed; 373 AA.
AC P06002; A0AVV9; Q4QPQ2; Q9TX56; Q9VDS8;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 01-MAY-2013, entry version 130.
DE RecName: Full=Opsin Rh1;
DE AltName: Full=Neither inactivation nor afterpotential E protein;
DE AltName: Full=Outer R1-R6 photoreceptor cells opsin;
GN Name=ninaE; Synonyms=Rh1; ORFNames=CG4550;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
OC Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha;
OC Ephydroidea; Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2985266; DOI=10.1016/0092-8674(85)90343-5;
RA O'Tousa J.E., Baehr W., Martin R.L., Hirsh J., Pak W.L.,
RA Applebury M.L.;
RT "The Drosophila ninaE gene encodes an opsin.";
RL Cell 40:839-850(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2580638; DOI=10.1016/0092-8674(85)90344-7;
RA Zuker C.S., Cowman A.F., Rubin G.M.;
RT "Isolation and structure of a rhodopsin gene from D. melanogaster.";
RL Cell 40:851-858(1985).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X.,
RA Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D.,
RA Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G.,
RA Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D.,
RA Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M.,
RA Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S.,
RA Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P.,
RA Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I.,
RA Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P.,
RA de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M.,
RA Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P.,
RA Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W.,
RA Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K.,
RA Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J.,
RA Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C.,
RA Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A.,
RA Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z.,
RA Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X.,
RA Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D.,
RA Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A.,
RA Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L.,
RA Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M.,
RA Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G.,
RA Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H.,
RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E.,
RA Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X.,
RA Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J.,
RA Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A.,
RA Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L.,
RA Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X.,
RA Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q.,
RA Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M.,
RA Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a
RT systematic review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W.,
RA Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E.,
RA George R.A., Gonzalez M., Guarin H., Kapadia B., Kronmiller B.,
RA Li P.W., Liao G., Miranda A., Mungall C.J., Nunoo J., Pacleb J.M.,
RA Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C.,
RA Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE OF 26-346.
RX PubMed=8006992; DOI=10.1007/BF00176087;
RA Carulli J.P., Chen D.M., Stark W.S., Hartl D.L.;
RT "Phylogeny and physiology of Drosophila opsins.";
RL J. Mol. Evol. 38:250-262(1994).
RN [7]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-196, AND MASS
RP SPECTROMETRY.
RC STRAIN=Oregon-R; TISSUE=Head;
RX PubMed=17893096; DOI=10.1093/glycob/cwm097;
RA Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L.,
RA Panin V.;
RT "Identification of N-glycosylated proteins from the central nervous
RT system of Drosophila melanogaster.";
RL Glycobiology 17:1388-1403(2007).
CC -!- FUNCTION: Visual pigments are the light-absorbing molecules that
CC mediate vision. They consist of an apoprotein, opsin, covalently
CC linked to cis-retinal.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Absorption:
CC Abs(max)=480 nm;
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- PTM: Phosphorylated on some or all of the serine and threonine
CC residues present in the C-terminal region.
CC -!- MISCELLANEOUS: Each Drosophila eye is composed of 800 facets or
CC ommatidia. Each ommatidium contains 8 photoreceptor cells (R1-R8),
CC the R1 to R6 cells are outer cells, while R7 and R8 are inner
CC cells.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC Opsin subfamily.
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DR EMBL; K02315; AAA28733.1; -; Genomic_DNA.
DR EMBL; K02320; AAA28735.1; ALT_SEQ; Genomic_DNA.
DR EMBL; K02316; AAA28735.1; JOINED; Genomic_DNA.
DR EMBL; K02317; AAA28735.1; JOINED; Genomic_DNA.
DR EMBL; K02318; AAA28735.1; JOINED; Genomic_DNA.
DR EMBL; K02319; AAA28735.1; JOINED; Genomic_DNA.
DR EMBL; AE014297; AAF55712.1; -; Genomic_DNA.
DR EMBL; BT010221; AAQ23539.1; -; mRNA.
DR EMBL; BT023714; AAY85114.1; -; mRNA.
DR EMBL; BT029277; ABK30914.1; -; mRNA.
DR PIR; A90864; OOFF.
DR RefSeq; NP_524407.1; NM_079683.2.
DR UniGene; Dm.6744; -.
DR ProteinModelPortal; P06002; -.
DR SMR; P06002; 12-369.
DR DIP; DIP-17500N; -.
DR IntAct; P06002; 1.
DR MINT; MINT-326982; -.
DR PaxDb; P06002; -.
DR PRIDE; P06002; -.
DR EnsemblMetazoa; FBtr0083857; FBpp0083266; FBgn0002940.
DR GeneID; 42367; -.
DR KEGG; dme:Dmel_CG4550; -.
DR CTD; 42367; -.
DR FlyBase; FBgn0002940; ninaE.
DR eggNOG; NOG255465; -.
DR GeneTree; ENSGT00700000104057; -.
DR InParanoid; P06002; -.
DR KO; K13802; -.
DR OMA; NTIWGAC; -.
DR OrthoDB; EOG451C6G; -.
DR PhylomeDB; P06002; -.
DR ChiTaRS; ninaE; drosophila.
DR GenomeRNAi; 42367; -.
DR NextBio; 828455; -.
DR Bgee; P06002; -.
DR GermOnline; CG4550; Drosophila melanogaster.
DR GO; GO:0016023; C:cytoplasmic membrane-bounded vesicle; IDA:FlyBase.
DR GO; GO:0016027; C:inaD signaling complex; IPI:FlyBase.
DR GO; GO:0016021; C:integral to membrane; ISS:FlyBase.
DR GO; GO:0005771; C:multivesicular body; IDA:FlyBase.
DR GO; GO:0005791; C:rough endoplasmic reticulum; IDA:FlyBase.
DR GO; GO:0005767; C:secondary lysosome; IDA:FlyBase.
DR GO; GO:0016029; C:subrhabdomeral cisterna; IDA:FlyBase.
DR GO; GO:0008020; F:G-protein coupled photoreceptor activity; ISS:FlyBase.
DR GO; GO:0008344; P:adult locomotory behavior; IMP:FlyBase.
DR GO; GO:0046673; P:negative regulation of compound eye retinal cell programmed cell death; IMP:FlyBase.
DR GO; GO:0008594; P:photoreceptor cell morphogenesis; IMP:FlyBase.
DR GO; GO:0007602; P:phototransduction; IMP:FlyBase.
DR GO; GO:0018298; P:protein-chromophore linkage; IEA:UniProtKB-KW.
DR GO; GO:0009642; P:response to light intensity; IMP:FlyBase.
DR GO; GO:0042052; P:rhabdomere development; IMP:FlyBase.
DR GO; GO:0043052; P:thermotaxis; IMP:FlyBase.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR001760; Opsin.
DR InterPro; IPR001735; Opsin_RH1/RH2.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00238; OPSIN.
DR PRINTS; PR00576; OPSINRH1RH2.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR PROSITE; PS00238; OPSIN; 1.
PE 1: Evidence at protein level;
KW Chromophore; Complete proteome; Disulfide bond;
KW G-protein coupled receptor; Glycoprotein; Membrane; Phosphoprotein;
KW Photoreceptor protein; Receptor; Reference proteome; Retinal protein;
KW Sensory transduction; Transducer; Transmembrane; Transmembrane helix;
KW Vision.
FT CHAIN 1 373 Opsin Rh1.
FT /FTId=PRO_0000197622.
FT TOPO_DOM 1 49 Extracellular.
FT TRANSMEM 50 74 Helical; Name=1; (Potential).
FT TOPO_DOM 75 86 Cytoplasmic.
FT TRANSMEM 87 112 Helical; Name=2; (Potential).
FT TOPO_DOM 113 126 Extracellular.
FT TRANSMEM 127 146 Helical; Name=3; (Potential).
FT TOPO_DOM 147 165 Cytoplasmic.
FT TRANSMEM 166 189 Helical; Name=4; (Potential).
FT TOPO_DOM 190 213 Extracellular.
FT TRANSMEM 214 241 Helical; Name=5; (Potential).
FT TOPO_DOM 242 276 Cytoplasmic.
FT TRANSMEM 277 300 Helical; Name=6; (Potential).
FT TOPO_DOM 301 307 Extracellular.
FT TRANSMEM 308 332 Helical; Name=7; (Potential).
FT TOPO_DOM 333 373 Cytoplasmic.
FT MOD_RES 319 319 N6-(retinylidene)lysine.
FT CARBOHYD 20 20 N-linked (GlcNAc...) (Probable).
FT CARBOHYD 196 196 N-linked (GlcNAc...).
FT DISULFID 123 200 Potential.
SQ SEQUENCE 373 AA; 41495 MW; FFF36C90DDD68294 CRC64;
MESFAVAAAQ LGPHFAPLSN GSVVDKVTPD MAHLISPYWN QFPAMDPIWA KILTAYMIMI
GMISWCGNGV VIYIFATTKS LRTPANLLVI NLAISDFGIM ITNTPMMGIN LYFETWVLGP
MMCDIYAGLG SAFGCSSIWS MCMISLDRYQ VIVKGMAGRP MTIPLALGKI AYIWFMSSIW
CLAPAFGWSR YVPEGNLTSC GIDYLERDWN PRSYLIFYSI FVYYIPLFLI CYSYWFIIAA
VSAHEKAMRE QAKKMNVKSL RSSEDAEKSA EGKLAKVALV TITLWFMAWT PYLVINCMGL
FKFEGLTPLN TIWGACFAKS AACYNPIVYG ISHPKYRLAL KEKCPCCVFG KVDDGKSSDA
QSQATASEAE SKA
//
