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Database: UniProt
Entry: P06223
LinkDB: P06223
Original site: P06223 
ID   RP54_KLEOX              Reviewed;         477 AA.
AC   P06223;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   01-OCT-2014, entry version 74.
DE   RecName: Full=RNA polymerase sigma-54 factor;
GN   Name=rpoN; Synonyms=ntrA;
OS   Klebsiella oxytoca.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Klebsiella.
OX   NCBI_TaxID=571;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2999700; DOI=10.1093/nar/13.21.7607;
RA   Merrick M.J., Gibbins J.R.;
RT   "The nucleotide sequence of the nitrogen-regulation gene ntrA of
RT   Klebsiella pneumoniae and comparison with conserved features in
RT   bacterial RNA polymerase sigma factors.";
RL   Nucleic Acids Res. 13:7607-7620(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 465-477.
RC   STRAIN=M5a1;
RX   PubMed=2695747; DOI=10.1111/j.1365-2958.1989.tb00162.x;
RA   Merrick M.J., Coppard J.R.;
RT   "Mutations in genes downstream of the rpoN gene (encoding sigma 54) of
RT   Klebsiella pneumoniae affect expression from sigma 54-dependent
RT   promoters.";
RL   Mol. Microbiol. 3:1765-1775(1989).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are
CC       then released. This sigma factor is responsible for the expression
CC       of the nitrogen fixation genes. The open complex (sigma-54 and
CC       core RNA polymerase) serves as the receptor for receipt of the
CC       melting signal from the remotely bound activator protein NifA for
CC       the expression of the nitrogen fixation proteins.
CC   -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR   EMBL; X03147; CAA26925.1; -; Genomic_DNA.
DR   EMBL; X16335; CAA34390.1; -; Genomic_DNA.
DR   PIR; A24115; A24115.
DR   ProteinModelPortal; P06223; -.
DR   PRIDE; P06223; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   InterPro; IPR000394; RNA_pol_sigma_54.
DR   InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR   InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR   Pfam; PF00309; Sigma54_AID; 1.
DR   Pfam; PF04963; Sigma54_CBD; 1.
DR   Pfam; PF04552; Sigma54_DBD; 1.
DR   PIRSF; PIRSF000774; RpoN; 1.
DR   PRINTS; PR00045; SIGMA54FCT.
DR   TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR   PROSITE; PS00717; SIGMA54_1; 1.
DR   PROSITE; PS00718; SIGMA54_2; 1.
DR   PROSITE; PS50044; SIGMA54_3; 1.
PE   3: Inferred from homology;
KW   DNA-binding; DNA-directed RNA polymerase; Nitrogen fixation;
KW   Nucleotidyltransferase; Sigma factor; Transcription;
KW   Transcription regulation; Transferase.
FT   CHAIN         1    477       RNA polymerase sigma-54 factor.
FT                                /FTId=PRO_0000205533.
FT   DNA_BIND    366    385       H-T-H motif. {ECO:0000250}.
FT   MOTIF       454    462       RPON box.
FT   COMPBIAS      3     39       Gln-rich.
SQ   SEQUENCE   477 AA;  53927 MW;  481F29B66B3DCF9A CRC64;
     MKQGLQLRLS QQLAMTPQLQ QAIRLLQLST LELQQELQQA LDSNPLLEQT DLHDEVETKE
     AEDRESLDTV DALEQKEMPE ELPLDASWDE IYTAGTPSGN GVDYQDDELP VYQGETTQSL
     QDYLMWQVEL TPFTDTDRAI ATSIVDAVDD TGYLTISVED IVESIGDDEI GLEEVEAVLK
     RIQRFDPVGV AAKDLRDCLL VQLSQFAKET PWIEEARLII SDHLDLLANH DFRSLMRVTR
     LKEEVLKEAV NLIQSLDPRP GQSIQTGEPE YVIPDVLVRK VNDRWVVELN SDSLPRLKIN
     QQYAAMGNST RNDADGQFIR SNLQEARWLI KSLESRNDTL LRVSRCIVEQ QQAFFEQGEE
     FMKPMVLADI AQAVEMHEST ISRVTTQKYL HSPRGIFELK YFFSSHVNTE GGGEASSTAI
     RALVKKLIAA ENPAKPLSDS KLTTMLSDQG IMVARRTVAK YRESLSIPPS NQRKQLV
//
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