ID RP54_KLEOX Reviewed; 477 AA.
AC P06223;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 03-APR-2013, entry version 72.
DE RecName: Full=RNA polymerase sigma-54 factor;
GN Name=rpoN; Synonyms=ntrA;
OS Klebsiella oxytoca.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Klebsiella.
OX NCBI_TaxID=571;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2999700; DOI=10.1093/nar/13.21.7607;
RA Merrick M.J., Gibbins J.R.;
RT "The nucleotide sequence of the nitrogen-regulation gene ntrA of
RT Klebsiella pneumoniae and comparison with conserved features in
RT bacterial RNA polymerase sigma factors.";
RL Nucleic Acids Res. 13:7607-7620(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 465-477.
RC STRAIN=M5a1;
RX PubMed=2695747; DOI=10.1111/j.1365-2958.1989.tb00162.x;
RA Merrick M.J., Coppard J.R.;
RT "Mutations in genes downstream of the rpoN gene (encoding sigma 54) of
RT Klebsiella pneumoniae affect expression from sigma 54-dependent
RT promoters.";
RL Mol. Microbiol. 3:1765-1775(1989).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are
CC then released. This sigma factor is responsible for the expression
CC of the nitrogen fixation genes. The open complex (sigma-54 and
CC core RNA polymerase) serves as the receptor for receipt of the
CC melting signal from the remotely bound activator protein NifA for
CC the expression of the nitrogen fixation proteins.
CC -!- SIMILARITY: Belongs to the sigma-54 factor family.
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DR EMBL; X03147; CAA26925.1; -; Genomic_DNA.
DR EMBL; X16335; CAA34390.1; -; Genomic_DNA.
DR PIR; A24115; A24115.
DR ProteinModelPortal; P06223; -.
DR PRIDE; P06223; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-dependent transcription, initiation; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR InterPro; IPR000394; RNA_pol_sigma_54.
DR InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR Pfam; PF00309; Sigma54_AID; 1.
DR Pfam; PF04963; Sigma54_CBD; 1.
DR Pfam; PF04552; Sigma54_DBD; 1.
DR PIRSF; PIRSF000774; RpoN; 1.
DR PRINTS; PR00045; SIGMA54FCT.
DR TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR PROSITE; PS00717; SIGMA54_1; 1.
DR PROSITE; PS00718; SIGMA54_2; 1.
DR PROSITE; PS50044; SIGMA54_3; 1.
PE 3: Inferred from homology;
KW DNA-binding; DNA-directed RNA polymerase; Nitrogen fixation;
KW Nucleotidyltransferase; Sigma factor; Transcription;
KW Transcription regulation; Transferase.
FT CHAIN 1 477 RNA polymerase sigma-54 factor.
FT /FTId=PRO_0000205533.
FT DNA_BIND 366 385 H-T-H motif (By similarity).
FT MOTIF 454 462 RPON box.
FT COMPBIAS 3 39 Gln-rich.
SQ SEQUENCE 477 AA; 53927 MW; 481F29B66B3DCF9A CRC64;
MKQGLQLRLS QQLAMTPQLQ QAIRLLQLST LELQQELQQA LDSNPLLEQT DLHDEVETKE
AEDRESLDTV DALEQKEMPE ELPLDASWDE IYTAGTPSGN GVDYQDDELP VYQGETTQSL
QDYLMWQVEL TPFTDTDRAI ATSIVDAVDD TGYLTISVED IVESIGDDEI GLEEVEAVLK
RIQRFDPVGV AAKDLRDCLL VQLSQFAKET PWIEEARLII SDHLDLLANH DFRSLMRVTR
LKEEVLKEAV NLIQSLDPRP GQSIQTGEPE YVIPDVLVRK VNDRWVVELN SDSLPRLKIN
QQYAAMGNST RNDADGQFIR SNLQEARWLI KSLESRNDTL LRVSRCIVEQ QQAFFEQGEE
FMKPMVLADI AQAVEMHEST ISRVTTQKYL HSPRGIFELK YFFSSHVNTE GGGEASSTAI
RALVKKLIAA ENPAKPLSDS KLTTMLSDQG IMVARRTVAK YRESLSIPPS NQRKQLV
//
