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Database: UniProt
Entry: P07026
LinkDB: P07026
Original site: P07026 
ID   SDIA_ECOLI              Reviewed;         240 AA.
AC   P07026; P76313;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   01-OCT-2014, entry version 121.
DE   RecName: Full=Regulatory protein SdiA;
GN   Name=sdiA; OrderedLocusNames=b1916, JW1901;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3515318; DOI=10.1093/nar/14.5.2301;
RA   Sharma S., Stark T.F., Beattie W.G., Moses R.E.;
RT   "Multiple control elements for the uvrC gene unit of Escherichia
RT   coli.";
RL   Nucleic Acids Res. 14:2301-2318(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA   Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA   Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA   Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA   Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA   Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C.,
RA   Yamamoto Y., Horiuchi T.;
RT   "A 460-kb DNA sequence of the Escherichia coli K-12 genome
RT   corresponding to the 40.1-50.0 min region on the linkage map.";
RL   DNA Res. 3:379-392(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   FUNCTION.
RX   PubMed=1915297;
RA   Wang X., de Boer P.A.J., Rothfield L.I.;
RT   "A factor that positively regulates cell division by activating
RT   transcription of the major cluster of essential cell division genes of
RT   Escherichia coli.";
RL   EMBO J. 10:3363-3372(1991).
RN   [6]
RP   ROLE IN YDIV REGULATION, REPRESSION BY GLUCOSE, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=K12 / W3110 / ZK126;
RX   PubMed=18560382; DOI=10.1038/cr.2008.67;
RA   Zhou X., Meng X., Sun B.;
RT   "An EAL domain protein and cyclic AMP contribute to the interaction
RT   between the two quorum sensing systems in Escherichia coli.";
RL   Cell Res. 18:937-948(2008).
RN   [7]
RP   STRUCTURE BY NMR OF 3-171 IN COMPLEX WITH AUTOINDUCER, AND BINDING OF
RP   HOMOSERINE LACTONE DERIVATIVES.
RX   PubMed=16307757; DOI=10.1016/j.jmb.2005.10.041;
RA   Yao Y., Martinez-Yamout M.A., Dickerson T.J., Brogan A.P.,
RA   Wright P.E., Dyson H.J.;
RT   "Structure of the Escherichia coli quorum sensing protein SdiA:
RT   activation of the folding switch by acyl homoserine lactones.";
RL   J. Mol. Biol. 355:262-273(2006).
CC   -!- FUNCTION: Activates cell division by specifically increasing
CC       transcription from one of the two promoters that lie immediately
CC       upstream of the ftsQAZ gene cluster. Activates ydiV expression in
CC       response to extracellular autoinducer AI-1 (Vibrio fischeri
CC       autoinducer oxoC6). {ECO:0000269|PubMed:18560382,
CC       ECO:0000269|PubMed:1915297}.
CC   -!- INDUCTION: Repressed by glucose. {ECO:0000269|PubMed:18560382}.
CC   -!- DOMAIN: Binding to the autoinducer occurs via the N-terminal 170
CC       residues; as E.coli does not produce LuxI autoinducers
CC       endogenously it should be able to bind to a number of different
CC       AI-1 autoinducers, which would enable it to detect other bacteria.
CC       This was shown to be the case.
CC   -!- DISRUPTION PHENOTYPE: A double sdiA/ydiV deletion mutant leads to
CC       decreased cAMP levels which inhibits quorum sensing system 2.
CC       {ECO:0000269|PubMed:18560382}.
CC   -!- SIMILARITY: Contains 1 HTH luxR-type DNA-binding domain.
CC       {ECO:0000255|PROSITE-ProRule:PRU00411}.
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DR   EMBL; X03691; CAA27327.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74983.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15736.1; -; Genomic_DNA.
DR   PIR; A64955; QQECU1.
DR   RefSeq; NP_416426.1; NC_000913.3.
DR   RefSeq; YP_490173.1; NC_007779.1.
DR   PDB; 2AVX; NMR; -; A=1-171.
DR   PDB; 4LFU; X-ray; 2.26 A; A=1-240.
DR   PDB; 4LGW; X-ray; 2.70 A; A=1-240.
DR   PDBsum; 2AVX; -.
DR   PDBsum; 4LFU; -.
DR   PDBsum; 4LGW; -.
DR   ProteinModelPortal; P07026; -.
DR   SMR; P07026; 5-240.
DR   IntAct; P07026; 8.
DR   STRING; 511145.b1916; -.
DR   PaxDb; P07026; -.
DR   PRIDE; P07026; -.
DR   EnsemblBacteria; AAC74983; AAC74983; b1916.
DR   EnsemblBacteria; BAA15736; BAA15736; BAA15736.
DR   GeneID; 12930608; -.
DR   GeneID; 946421; -.
DR   KEGG; ecj:Y75_p1887; -.
DR   KEGG; eco:b1916; -.
DR   PATRIC; 32119167; VBIEscCol129921_1998.
DR   EchoBASE; EB0928; -.
DR   EcoGene; EG10935; sdiA.
DR   eggNOG; COG2771; -.
DR   HOGENOM; HOG000111053; -.
DR   KO; K07782; -.
DR   OMA; VMPPEMK; -.
DR   OrthoDB; EOG6P8TQG; -.
DR   PhylomeDB; P07026; -.
DR   BioCyc; EcoCyc:PD02198; -.
DR   BioCyc; ECOL316407:JW1901-MONOMER; -.
DR   EvolutionaryTrace; P07026; -.
DR   PRO; PR:P07026; -.
DR   Genevestigator; P07026; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0032467; P:positive regulation of cytokinesis; IMP:EcoCyc.
DR   GO; GO:2000144; P:positive regulation of DNA-templated transcription, initiation; IDA:EcoCyc.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.30.450.80; -; 1.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR005143; TF_LuxR_autoind-bd_dom.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   InterPro; IPR011991; WHTH_DNA-bd_dom.
DR   Pfam; PF03472; Autoind_bind; 1.
DR   Pfam; PF00196; GerE; 1.
DR   PRINTS; PR00038; HTHLUXR.
DR   SMART; SM00421; HTH_LUXR; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF75516; SSF75516; 1.
DR   PROSITE; PS00622; HTH_LUXR_1; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Cell cycle; Cell division; Complete proteome;
KW   DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN         1    240       Regulatory protein SdiA.
FT                                /FTId=PRO_0000184187.
FT   DOMAIN      173    238       HTH luxR-type. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00411}.
FT   DNA_BIND    197    216       H-T-H motif. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00411}.
FT   CONFLICT    120    128       TQYLMLPNR -> HSVFNAAQTG (in Ref. 1;
FT                                CAA27327). {ECO:0000305}.
FT   CONFLICT    140    140       A -> R (in Ref. 1; CAA27327).
FT                                {ECO:0000305}.
FT   HELIX         6     19
FT   HELIX        23     36
FT   STRAND       40     47
FT   STRAND       51     54
FT   STRAND       57     61
FT   HELIX        65     73
FT   HELIX        76     78
FT   HELIX        81     83
FT   HELIX        85     87
FT   STRAND       92     94
FT   HELIX        97    100
FT   HELIX       104    112
FT   STRAND      117    124
FT   STRAND      130    138
FT   HELIX       147    167
FT   TURN        171    173
FT   HELIX       182    192
FT   HELIX       197    204
FT   HELIX       208    222
FT   HELIX       227    236
SQ   SEQUENCE   240 AA;  28117 MW;  C04CCC50C06135C4 CRC64;
     MQDKDFFSWR RTMLLRFQRM ETAEEVYHEI ELQAQQLEYD YYSLCVRHPV PFTRPKVAFY
     TNYPEAWVSY YQAKNFLAID PVLNPENFSQ GHLMWNDDLF SEAQPLWEAA RAHGLRRGVT
     QYLMLPNRAL GFLSFSRCSA REIPILSDEL QLKMQLLVRE SLMALMRLND EIVMTPEMNF
     SKREKEILRW TAEGKTSAEI AMILSISENT VNFHQKNMQK KINAPNKTQV ACYAAATGLI
//
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