ID RP54_AZOVI Reviewed; 502 AA.
AC P08623;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 03-APR-2013, entry version 66.
DE RecName: Full=RNA polymerase sigma-54 factor;
GN Name=rpoN; Synonyms=ntrA;
OS Azotobacter vinelandii.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Azotobacter.
OX NCBI_TaxID=354;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW;
RX PubMed=3481423; DOI=10.1007/BF00325701;
RA Merrick M.J., Gibbins J., Toukdarian A.;
RT "The nucleotide sequence of the sigma factor gene ntrA (rpoN) of
RT Azotobacter vinelandii: analysis of conserved sequences in NtrA
RT proteins.";
RL Mol. Gen. Genet. 210:323-330(1987).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are
CC then released. This sigma factor is responsible for the expression
CC of the nitrogen fixation genes. The open complex (sigma-54 and
CC core RNA polymerase) serves as the receptor for receipt of the
CC melting signal from the remotely bound activator protein NifA for
CC the expression of the nitrogen fixation proteins.
CC -!- SIMILARITY: Belongs to the sigma-54 factor family.
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DR EMBL; X05888; CAA29314.1; -; Genomic_DNA.
DR PIR; S00720; S00720.
DR ProteinModelPortal; P08623; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-dependent transcription, initiation; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR InterPro; IPR000394; RNA_pol_sigma_54.
DR InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR Pfam; PF00309; Sigma54_AID; 1.
DR Pfam; PF04963; Sigma54_CBD; 1.
DR Pfam; PF04552; Sigma54_DBD; 1.
DR PIRSF; PIRSF000774; RpoN; 1.
DR PRINTS; PR00045; SIGMA54FCT.
DR TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR PROSITE; PS00717; SIGMA54_1; 1.
DR PROSITE; PS00718; SIGMA54_2; 1.
DR PROSITE; PS50044; SIGMA54_3; 1.
PE 3: Inferred from homology;
KW DNA-binding; DNA-directed RNA polymerase; Nitrogen fixation;
KW Nucleotidyltransferase; Sigma factor; Transcription;
KW Transcription regulation; Transferase.
FT CHAIN 1 502 RNA polymerase sigma-54 factor.
FT /FTId=PRO_0000205523.
FT DNA_BIND 391 410 H-T-H motif (Potential).
FT MOTIF 479 487 RPON box.
FT COMPBIAS 11 38 Gln-rich.
SQ SEQUENCE 502 AA; 56917 MW; 699A405BE87D4F10 CRC64;
MKPSLVLKMG QQLTMTPQLQ QAIRLLQLST LDLQQEIQEA LDSNPMLERQ EDAEDYDSPD
MLGEHGDQST LDTTPGSYQE GYESGAASED GGTLEEGDWH ERIPSELPVD TAWEDIYQTS
ASNLPSTDED EWDFTTRTST GESLQSHLLW QLNLTPMSDT DRLIAVTLID SINSDGYLEA
ALEEILASLD PELGVELDEV EMVLRRIQQF EPAGIAARDL SESLLLQLRQ LPPDTPWLEE
AKRLAKDYLD LLGNRDFTQL MRRMKLKEEE LRPVIELIQS LNPRPGAQIE SSEPEYVVPD
VIVRKHNDRW LVELNQEAVP RLRINPHYAG FIRRADASAD NTFMRNQLQE ARWFIKSLQS
RNETLMKVST QIVEHQRGFL DYGEEAMKPL VLHDIAEAVG MHESTISRVT TQKYMHTPRG
IYELKYFFSS HVSTAEGGEC SSTAIRAIIK KLIAAENPKK PLSDSKIAGL LEEQGIQVAR
RTVAKYRESL SIAPSSERKR LM
//
