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Database: UniProt
Entry: P08735
LinkDB: P08735
Original site: P08735 
ID   G3PC1_MAIZE             Reviewed;         337 AA.
AC   P08735;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 2.
DT   29-OCT-2014, entry version 108.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase 1, cytosolic;
DE            EC=1.2.1.12;
GN   Name=GAPC1; Synonyms=GAPC, GPC1;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae;
OC   PACMAD clade; Panicoideae; Andropogoneae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Wisconsin 22;
RX   PubMed=2810356; DOI=10.1016/0022-2836(89)90147-2;
RA   Martinez P., Martin W.F., Cerff R.;
RT   "Structure, evolution and anaerobic regulation of a nuclear gene
RT   encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase from
RT   maize.";
RL   J. Mol. Biol. 208:551-565(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=3131533; DOI=10.1007/BF02101150;
RA   Brinkmann H., Martinez P., Quigley F., Martin W.F., Cerff R.;
RT   "Endosymbiotic origin and codon bias of the nuclear gene for
RT   chloroplast glyceraldehyde-3-phosphate dehydrogenase from maize.";
RL   J. Mol. Evol. 26:320-328(1987).
CC   -!- FUNCTION: Key enzyme in glycolysis that catalyzes the first step
CC       of the pathway by converting D-glyceraldehyde 3-phosphate (G3P)
CC       into 3-phospho-D-glyceroyl phosphate. Essential for the
CC       maintenance of cellular ATP levels and carbohydrate metabolism (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC       {ECO:0000255|PROSITE-ProRule:PRU10009}.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 1/5.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- MISCELLANEOUS: Plants contain two types of GAPDH: cytosolic forms
CC       which participate in glycolysis and chloroplast forms which
CC       participate in photosynthesis. All the forms are encoded by
CC       distinct genes.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family. {ECO:0000305}.
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DR   EMBL; X07156; CAA30151.1; -; mRNA.
DR   EMBL; X15596; CAA33620.1; -; Genomic_DNA.
DR   PIR; S00354; DEZMGC.
DR   RefSeq; NP_001105413.1; NM_001111943.1.
DR   UniGene; Zm.3765; -.
DR   ProteinModelPortal; P08735; -.
DR   SMR; P08735; 3-333.
DR   PRIDE; P08735; -.
DR   ProMEX; P08735; -.
DR   GeneID; 542367; -.
DR   KEGG; zma:542367; -.
DR   Gramene; P08735; -.
DR   MaizeGDB; 13873; -.
DR   HOGENOM; HOG000071678; -.
DR   KO; K00134; -.
DR   UniPathway; UPA00109; UER00184.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IEA:UniProtKB-EC.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0006006; P:glucose metabolic process; IEA:InterPro.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.720; -; 1.
DR   InterPro; IPR020831; GlycerAld/Erythrose_P_DH.
DR   InterPro; IPR020830; GlycerAld_3-P_DH_AS.
DR   InterPro; IPR020829; GlycerAld_3-P_DH_cat.
DR   InterPro; IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DH_1.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   PANTHER; PTHR10836; PTHR10836; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   SMART; SM00846; Gp_dh_N; 1.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT   CHAIN         1    337       Glyceraldehyde-3-phosphate dehydrogenase
FT                                1, cytosolic.
FT                                /FTId=PRO_0000145605.
FT   NP_BIND      13     14       NAD. {ECO:0000250}.
FT   REGION        1    151       Binding to NAD.
FT   REGION      152    337       Catalytic.
FT   REGION      153    155       Glyceraldehyde 3-phosphate binding.
FT                                {ECO:0000250}.
FT   REGION      213    214       Glyceraldehyde 3-phosphate binding.
FT                                {ECO:0000250}.
FT   ACT_SITE    154    154       Nucleophile. {ECO:0000255|PROSITE-
FT                                ProRule:PRU10009}.
FT   BINDING      35     35       NAD. {ECO:0000250}.
FT   BINDING      82     82       NAD; via carbonyl oxygen. {ECO:0000250}.
FT   BINDING     184    184       Glyceraldehyde 3-phosphate.
FT                                {ECO:0000250}.
FT   BINDING     236    236       Glyceraldehyde 3-phosphate.
FT                                {ECO:0000250}.
FT   BINDING     318    318       NAD. {ECO:0000250}.
FT   SITE        181    181       Activates thiol group during catalysis.
FT                                {ECO:0000250}.
FT   CONFLICT    336    336       T -> S (in Ref. 2; CAA30151).
FT                                {ECO:0000305}.
SQ   SEQUENCE   337 AA;  36523 MW;  E18F580F09FDC07B CRC64;
     MGKIKIGING FGRIGRLVAR VALQSEDVEL VAVNDPFITT DYMTYMFKYD TVHGHWKHSD
     ITLKDSKTLL FGDKPVTVFG IRNPEEIPWG EAGAEYVVES TGVFTDKDKA AAHLKGGAKK
     VVISAPSKDA PMFVVGVNED KYTSDVNIVS NASCTTNCLA PLAKVIHDNF GIVEGLMTTV
     HAITATQKTV DGPSAKDWRG GRAASFNIIP SSTGAAKAVG KVLPDLNGKL TGMSFRVPTV
     DVSVVDLTVR IEKGASYEDI KKAIKAASEG PLKGIMGYVE EDLVSTDFLG DSRSSIFDAK
     AGIALNDHFV KLVSWYDNEW GYSNRVVDLI RHMFKTQ
//
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