ID G3PC1_MAIZE Reviewed; 337 AA.
AC P08735;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 2.
DT 03-APR-2013, entry version 102.
DE RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase 1, cytosolic;
DE EC=1.2.1.12;
GN Name=GAPC1; Synonyms=GAPC, GPC1;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae;
OC PACMAD clade; Panicoideae; Andropogoneae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Wisconsin 22;
RX PubMed=2810356; DOI=10.1016/0022-2836(89)90147-2;
RA Martinez P., Martin W.F., Cerff R.;
RT "Structure, evolution and anaerobic regulation of a nuclear gene
RT encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase from
RT maize.";
RL J. Mol. Biol. 208:551-565(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=3131533; DOI=10.1007/BF02101150;
RA Brinkmann H., Martinez P., Quigley F., Martin W.F., Cerff R.;
RT "Endosymbiotic origin and codon bias of the nuclear gene for
RT chloroplast glyceraldehyde-3-phosphate dehydrogenase from maize.";
RL J. Mol. Evol. 26:320-328(1987).
CC -!- FUNCTION: Key enzyme in glycolysis that catalyzes the first step
CC of the pathway by converting D-glyceraldehyde 3-phosphate (G3P)
CC into 3-phospho-D-glyceroyl phosphate. Essential for the
CC maintenance of cellular ATP levels and carbohydrate metabolism (By
CC similarity).
CC -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC glyceraldehyde 3-phosphate: step 1/5.
CC -!- SUBUNIT: Homotetramer (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- MISCELLANEOUS: Plants contain two types of GAPDH: cytosolic forms
CC which participate in glycolysis and chloroplast forms which
CC participate in photosynthesis. All the forms are encoded by
CC distinct genes.
CC -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC dehydrogenase family.
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DR EMBL; X07156; CAA30151.1; -; mRNA.
DR EMBL; X15596; CAA33620.1; -; Genomic_DNA.
DR PIR; S00354; DEZMGC.
DR RefSeq; NP_001105413.1; NM_001111943.1.
DR UniGene; Zm.3765; -.
DR ProteinModelPortal; P08735; -.
DR SMR; P08735; 3-333.
DR PRIDE; P08735; -.
DR ProMEX; P08735; -.
DR GeneID; 542367; -.
DR KEGG; zma:542367; -.
DR Gramene; P08735; -.
DR MaizeGDB; 13873; -.
DR HOGENOM; HOG000071678; -.
DR KO; K00134; -.
DR UniPathway; UPA00109; UER00184.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IEA:EC.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.720; -; 1.
DR InterPro; IPR020831; GlycerAld/Erythrose_P_DH.
DR InterPro; IPR020830; GlycerAld_3-P_DH_AS.
DR InterPro; IPR020829; GlycerAld_3-P_DH_cat.
DR InterPro; IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
DR InterPro; IPR006424; Glyceraldehyde-3-P_DH_1.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR PANTHER; PTHR10836; PTHR10836; 1.
DR Pfam; PF02800; Gp_dh_C; 1.
DR Pfam; PF00044; Gp_dh_N; 1.
DR PIRSF; PIRSF000149; GAP_DH; 1.
DR PRINTS; PR00078; G3PDHDRGNASE.
DR SMART; SM00846; Gp_dh_N; 1.
DR TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR PROSITE; PS00071; GAPDH; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT CHAIN 1 337 Glyceraldehyde-3-phosphate dehydrogenase
FT 1, cytosolic.
FT /FTId=PRO_0000145605.
FT NP_BIND 13 14 NAD (By similarity).
FT REGION 1 151 Binding to NAD.
FT REGION 152 337 Catalytic.
FT REGION 153 155 Glyceraldehyde 3-phosphate binding (By
FT similarity).
FT REGION 213 214 Glyceraldehyde 3-phosphate binding (By
FT similarity).
FT ACT_SITE 154 154 Nucleophile (By similarity).
FT BINDING 35 35 NAD (By similarity).
FT BINDING 82 82 NAD; via carbonyl oxygen (By similarity).
FT BINDING 184 184 Glyceraldehyde 3-phosphate (By
FT similarity).
FT BINDING 236 236 Glyceraldehyde 3-phosphate (By
FT similarity).
FT BINDING 318 318 NAD (By similarity).
FT SITE 181 181 Activates thiol group during catalysis
FT (By similarity).
FT CONFLICT 336 336 T -> S (in Ref. 2; CAA30151).
SQ SEQUENCE 337 AA; 36523 MW; E18F580F09FDC07B CRC64;
MGKIKIGING FGRIGRLVAR VALQSEDVEL VAVNDPFITT DYMTYMFKYD TVHGHWKHSD
ITLKDSKTLL FGDKPVTVFG IRNPEEIPWG EAGAEYVVES TGVFTDKDKA AAHLKGGAKK
VVISAPSKDA PMFVVGVNED KYTSDVNIVS NASCTTNCLA PLAKVIHDNF GIVEGLMTTV
HAITATQKTV DGPSAKDWRG GRAASFNIIP SSTGAAKAVG KVLPDLNGKL TGMSFRVPTV
DVSVVDLTVR IEKGASYEDI KKAIKAASEG PLKGIMGYVE EDLVSTDFLG DSRSSIFDAK
AGIALNDHFV KLVSWYDNEW GYSNRVVDLI RHMFKTQ
//
