ID MLE_DICDI Reviewed; 150 AA.
AC P09402; Q54Z15;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 2.
DT 24-JAN-2024, entry version 128.
DE RecName: Full=Myosin, essential light chain;
DE AltName: Full=EMLC;
DE AltName: Full=Myosin light chain alkali;
GN Name=mlcE; Synonyms=mlcB; ORFNames=DDB_G0277859;
OS Dictyostelium discoideum (Social amoeba).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2451126; DOI=10.1128/mcb.8.2.794-801.1988;
RA Chisholm R.L., Rushforth A.M., Pollenz R.S., Kuczmarski E.R., Tafuri S.R.;
RT "Dictyostelium discoideum myosin: isolation and characterization of cDNAs
RT encoding the essential light chain.";
RL Mol. Cell. Biol. 8:794-801(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SEQUENCE REVISION.
RC STRAIN=AX3;
RX PubMed=1751970; DOI=10.1002/cm.970200202;
RA Pollenz R.S., Chisholm R.L.;
RT "Dictyostelium discoideum essential myosin light chain: gene structure and
RT characterization.";
RL Cell Motil. Cytoskeleton 20:83-94(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains (two
CC regulatory light chains and two essential light chains).
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M19337; AAA33228.1; ALT_SEQ; mRNA.
DR EMBL; X54161; CAA38100.1; -; Genomic_DNA.
DR EMBL; AAFI02000023; EAL68102.1; -; Genomic_DNA.
DR PIR; A28127; A28127.
DR RefSeq; XP_642037.1; XM_636945.1.
DR AlphaFoldDB; P09402; -.
DR SMR; P09402; -.
DR STRING; 44689.P09402; -.
DR SWISS-2DPAGE; P09402; -.
DR PaxDb; 44689-DDB0214813; -.
DR EnsemblProtists; EAL68102; EAL68102; DDB_G0277859.
DR GeneID; 8621248; -.
DR KEGG; ddi:DDB_G0277859; -.
DR dictyBase; DDB_G0277859; mlcE.
DR eggNOG; KOG0027; Eukaryota.
DR HOGENOM; CLU_061288_2_2_1; -.
DR InParanoid; P09402; -.
DR OMA; QECFSIF; -.
DR PhylomeDB; P09402; -.
DR Reactome; R-DDI-5627123; RHO GTPases activate PAKs.
DR PRO; PR:P09402; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0042641; C:actomyosin; IDA:dictyBase.
DR GO; GO:0031012; C:extracellular matrix; HDA:dictyBase.
DR GO; GO:0016460; C:myosin II complex; IDA:dictyBase.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0140660; F:cytoskeletal motor activator activity; IDA:dictyBase.
DR GO; GO:0000146; F:microfilament motor activity; IDA:dictyBase.
DR GO; GO:0032036; F:myosin heavy chain binding; IDA:dictyBase.
DR GO; GO:0016477; P:cell migration; IMP:dictyBase.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:dictyBase.
DR GO; GO:0031034; P:myosin filament assembly; IDA:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR CDD; cd00051; EFh; 2.
DR Gene3D; 1.10.238.10; EF-hand; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR PANTHER; PTHR23048:SF0; FI08416P-RELATED; 1.
DR PANTHER; PTHR23048; MYOSIN LIGHT CHAIN 1, 3; 1.
DR Pfam; PF13405; EF-hand_6; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; EF-hand; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 2: Evidence at transcript level;
KW Calcium; Metal-binding; Motor protein; Myosin; Reference proteome; Repeat.
FT CHAIN 1..150
FT /note="Myosin, essential light chain"
FT /id="PRO_0000198721"
FT DOMAIN 3..38
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 75..110
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 16
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 18
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 20
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 22
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 27
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ SEQUENCE 150 AA; 16246 MW; 5B6A47C2402FA2CC CRC64;
MSASADQIQE CFSIFDKDND GKVSVEDIGA CLRSLGKSPT MADIEALKTE IGAKEFDINT
LKSIYKKPNI KTPQEQQKEM LDAFKALDKE GHGTIQGAEL RQLLTTLGDY LSTAEVDELF
KEISVDSTTG AVSYASLVNT IVSGYPLGGF
//
