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Database: UniProt
Entry: P0A7K6
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ID   RL19_ECOLI              Reviewed;         115 AA.
AC   P0A7K6; P02420;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   01-OCT-2014, entry version 97.
DE   RecName: Full=50S ribosomal protein L19;
GN   Name=rplS; OrderedLocusNames=b2606, JW2587;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND OPERON STRUCTURE.
RC   STRAIN=K12;
RX   PubMed=6357787;
RA   Bystroem A.S., Hjalmarsson K.J., Wikstroem P.M., Bjoerk G.R.;
RT   "The nucleotide sequence of an Escherichia coli operon containing
RT   genes for the tRNA(m1G)methyltransferase, the ribosomal proteins S16
RT   and L19 and a 21-K polypeptide.";
RL   EMBO J. 2:899-905(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA   Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K.,
RA   Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N.,
RA   Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H.,
RA   Oshima T., Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S.,
RA   Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C.,
RA   Yamagata S., Horiuchi T.;
RT   "Construction of a contiguous 874-kb sequence of the Escherichia coli-
RT   K12 genome corresponding to 50.0-68.8 min on the linkage map and
RT   analysis of its sequence features.";
RL   DNA Res. 4:91-113(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   PROTEIN SEQUENCE OF 2-115.
RX   PubMed=339951; DOI=10.1021/bi00596a021;
RA   Brosius J., Arfsten U.;
RT   "Primary structure of protein L19 from the large subunit of
RT   Escherichia coli ribosomes.";
RL   Biochemistry 17:508-516(1978).
RN   [6]
RP   CROSS-LINKING TO L14.
RX   PubMed=2665813; DOI=10.1021/bi00435a071;
RA   Walleczek J., Martin T., Redl B., Stoeffler-Meilicke M., Stoeffler G.;
RT   "Comparative cross-linking study on the 50S ribosomal subunit from
RT   Escherichia coli.";
RL   Biochemistry 28:4099-4105(1989).
RN   [7]
RP   MASS SPECTROMETRY.
RC   STRAIN=K12 / ATCC 25404 / DSM 5698 / NCIMB 11290;
RX   PubMed=10094780; DOI=10.1006/abio.1998.3077;
RA   Arnold R.J., Reilly J.P.;
RT   "Observation of Escherichia coli ribosomal proteins and their
RT   posttranslational modifications by mass spectrometry.";
RL   Anal. Biochem. 269:105-112(1999).
RN   [8]
RP   STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS), AND INTERSUBUNIT
RP   BRIDGE FORMATION.
RC   STRAIN=MRE-600;
RX   PubMed=12809609; DOI=10.1016/S0092-8674(03)00427-6;
RA   Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M.,
RA   Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S.,
RA   Frank J.;
RT   "Study of the structural dynamics of the E. coli 70S ribosome using
RT   real-space refinement.";
RL   Cell 113:789-801(2003).
RN   [9]
RP   X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME
RP   STRUCTURES.
RC   STRAIN=MRE-600;
RX   PubMed=16272117; DOI=10.1126/science.1117230;
RA   Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W.,
RA   Vila-Sanjurjo A., Holton J.M., Cate J.H.D.;
RT   "Structures of the bacterial ribosome at 3.5 A resolution.";
RL   Science 310:827-834(2005).
CC   -!- FUNCTION: This protein is located at the 30S-50S ribosomal subunit
CC       interface. In the 70S ribosome (PubMed:12809609) it has been
CC       modeled to make two contacts with the 16S rRNA of the 30S subunit
CC       forming part of bridges B6 and B8. In the 3.5 A resolved
CC       structures (PubMed:16272117) L14 and L19 interact and together
CC       make contact with the 16S rRNA. The protein conformation is quite
CC       different between the 50S and 70S structures, which may be
CC       necessary for translocation. {ECO:0000269|PubMed:12809609,
CC       ECO:0000269|PubMed:16272117}.
CC   -!- SUBUNIT: Part of the 50S ribosomal subunit. Contacts L14
CC       (PubMed:2665813 and PubMed:16272117). Forms two bridges to the 30S
CC       subunit in the 70S ribosome, contacting the 16S rRNA.
CC   -!- INTERACTION:
CC       P0A761:nanE; NbExp=1; IntAct=EBI-543891, EBI-561432;
CC   -!- INDUCTION: Part of the rpsP-rimM-trmD-rplS operon.
CC   -!- MASS SPECTROMETRY: Mass=13001.7; Method=MALDI; Range=2-115;
CC       Evidence={ECO:0000269|PubMed:10094780};
CC   -!- SIMILARITY: Belongs to the ribosomal protein L19P family.
CC       {ECO:0000305}.
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DR   EMBL; X01818; CAA25960.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75655.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA16491.1; -; Genomic_DNA.
DR   PIR; S07951; R5EC19.
DR   RefSeq; NP_417097.1; NC_000913.3.
DR   RefSeq; YP_490829.1; NC_007779.1.
DR   PDB; 1P85; EM; 12.30 A; N=2-115.
DR   PDB; 1P86; EM; 11.50 A; N=2-115.
DR   PDB; 1VS6; X-ray; 3.46 A; P=1-115.
DR   PDB; 1VS8; X-ray; 3.46 A; P=1-115.
DR   PDB; 1VT2; X-ray; 3.30 A; P=1-115.
DR   PDB; 2AW4; X-ray; 3.46 A; P=2-115.
DR   PDB; 2AWB; X-ray; 3.46 A; P=2-115.
DR   PDB; 2GYA; EM; 15.00 A; N=2-115.
DR   PDB; 2GYC; EM; 15.00 A; N=2-115.
DR   PDB; 2I2T; X-ray; 3.22 A; P=2-115.
DR   PDB; 2I2V; X-ray; 3.22 A; P=2-115.
DR   PDB; 2J28; EM; 8.00 A; P=2-115.
DR   PDB; 2QAM; X-ray; 3.21 A; P=2-115.
DR   PDB; 2QAO; X-ray; 3.21 A; P=2-115.
DR   PDB; 2QBA; X-ray; 3.54 A; P=2-115.
DR   PDB; 2QBC; X-ray; 3.54 A; P=2-115.
DR   PDB; 2QBE; X-ray; 3.30 A; P=2-115.
DR   PDB; 2QBG; X-ray; 3.30 A; P=2-115.
DR   PDB; 2QBI; X-ray; 4.00 A; P=2-115.
DR   PDB; 2QBK; X-ray; 4.00 A; P=2-115.
DR   PDB; 2QOV; X-ray; 3.93 A; P=2-115.
DR   PDB; 2QOX; X-ray; 3.93 A; P=2-115.
DR   PDB; 2QOZ; X-ray; 3.50 A; P=2-115.
DR   PDB; 2QP1; X-ray; 3.50 A; P=2-115.
DR   PDB; 2RDO; EM; 9.10 A; P=2-115.
DR   PDB; 2VHM; X-ray; 3.74 A; P=2-115.
DR   PDB; 2VHN; X-ray; 3.74 A; P=2-115.
DR   PDB; 2WWQ; EM; 5.80 A; P=2-115.
DR   PDB; 2Z4L; X-ray; 4.45 A; P=2-115.
DR   PDB; 2Z4N; X-ray; 4.45 A; P=2-115.
DR   PDB; 3BBX; EM; 10.00 A; P=2-115.
DR   PDB; 3DF2; X-ray; 3.50 A; P=2-114.
DR   PDB; 3DF4; X-ray; 3.50 A; P=2-114.
DR   PDB; 3E1B; EM; -; I=1-115.
DR   PDB; 3E1D; EM; -; I=1-115.
DR   PDB; 3FIK; EM; 6.70 A; P=2-115.
DR   PDB; 3I1N; X-ray; 3.19 A; P=1-115.
DR   PDB; 3I1P; X-ray; 3.19 A; P=1-115.
DR   PDB; 3I1R; X-ray; 3.81 A; P=1-115.
DR   PDB; 3I1T; X-ray; 3.81 A; P=1-115.
DR   PDB; 3I20; X-ray; 3.71 A; P=1-115.
DR   PDB; 3I22; X-ray; 3.71 A; P=1-115.
DR   PDB; 3IZT; EM; -; Q=1-115.
DR   PDB; 3IZU; EM; -; Q=1-115.
DR   PDB; 3J01; EM; -; P=2-115.
DR   PDB; 3J0T; EM; 12.10 A; R=2-115.
DR   PDB; 3J0W; EM; 14.70 A; R=2-115.
DR   PDB; 3J0Y; EM; 13.50 A; R=2-115.
DR   PDB; 3J11; EM; 13.10 A; R=2-115.
DR   PDB; 3J12; EM; 11.50 A; R=2-115.
DR   PDB; 3J14; EM; 11.50 A; R=2-115.
DR   PDB; 3J19; EM; 8.30 A; P=2-115.
DR   PDB; 3J37; EM; 9.80 A; T=2-115.
DR   PDB; 3J4X; EM; 12.00 A; P=1-115.
DR   PDB; 3J50; EM; 20.00 A; P=1-115.
DR   PDB; 3J51; EM; 17.00 A; P=1-115.
DR   PDB; 3J52; EM; 12.00 A; P=1-115.
DR   PDB; 3J54; EM; 13.00 A; P=1-115.
DR   PDB; 3J56; EM; 15.00 A; P=1-115.
DR   PDB; 3J58; EM; 17.00 A; P=1-115.
DR   PDB; 3J5A; EM; 12.00 A; P=1-115.
DR   PDB; 3J5C; EM; 17.00 A; P=1-115.
DR   PDB; 3J5E; EM; 17.00 A; P=1-115.
DR   PDB; 3J5G; EM; 20.00 A; P=1-115.
DR   PDB; 3J5I; EM; 15.00 A; P=1-115.
DR   PDB; 3J5K; EM; 9.00 A; P=1-115.
DR   PDB; 3J5L; EM; 6.60 A; P=2-115.
DR   PDB; 3J5O; EM; 6.80 A; P=1-115.
DR   PDB; 3J5U; EM; 7.60 A; R=2-115.
DR   PDB; 3J5W; EM; 7.60 A; S=2-115.
DR   PDB; 3KCR; EM; -; P=1-115.
DR   PDB; 3OAS; X-ray; 3.25 A; P=2-115.
DR   PDB; 3OAT; X-ray; 3.25 A; P=2-115.
DR   PDB; 3OFC; X-ray; 3.19 A; P=2-115.
DR   PDB; 3OFD; X-ray; 3.19 A; P=2-115.
DR   PDB; 3OFQ; X-ray; 3.10 A; P=2-115.
DR   PDB; 3OFR; X-ray; 3.10 A; P=2-115.
DR   PDB; 3OFZ; X-ray; 3.29 A; P=2-115.
DR   PDB; 3OG0; X-ray; 3.29 A; P=2-115.
DR   PDB; 3ORB; X-ray; 3.30 A; P=1-115.
DR   PDB; 3R8S; X-ray; 3.00 A; P=2-115.
DR   PDB; 3R8T; X-ray; 3.00 A; P=2-115.
DR   PDB; 3SGF; X-ray; 3.20 A; T=1-115.
DR   PDB; 3UOS; X-ray; 3.70 A; T=1-115.
DR   PDB; 4CSU; EM; 5.50 A; P=2-115.
DR   PDB; 4GAR; X-ray; 3.30 A; P=1-115.
DR   PDB; 4GAU; X-ray; 3.30 A; P=1-115.
DR   PDB; 4KIX; X-ray; 2.90 A; P=1-115.
DR   PDB; 4KIZ; X-ray; 2.90 A; P=1-115.
DR   PDB; 4KJ1; X-ray; 2.90 A; P=1-115.
DR   PDB; 4KJ3; X-ray; 2.90 A; P=1-115.
DR   PDB; 4KJ5; X-ray; 2.90 A; P=1-115.
DR   PDB; 4KJ7; X-ray; 2.90 A; P=1-115.
DR   PDB; 4KJ9; X-ray; 2.90 A; P=1-115.
DR   PDB; 4KJB; X-ray; 2.90 A; P=1-115.
DR   PDBsum; 1P85; -.
DR   PDBsum; 1P86; -.
DR   PDBsum; 1VS6; -.
DR   PDBsum; 1VS8; -.
DR   PDBsum; 1VT2; -.
DR   PDBsum; 2AW4; -.
DR   PDBsum; 2AWB; -.
DR   PDBsum; 2GYA; -.
DR   PDBsum; 2GYC; -.
DR   PDBsum; 2I2T; -.
DR   PDBsum; 2I2V; -.
DR   PDBsum; 2J28; -.
DR   PDBsum; 2QAM; -.
DR   PDBsum; 2QAO; -.
DR   PDBsum; 2QBA; -.
DR   PDBsum; 2QBC; -.
DR   PDBsum; 2QBE; -.
DR   PDBsum; 2QBG; -.
DR   PDBsum; 2QBI; -.
DR   PDBsum; 2QBK; -.
DR   PDBsum; 2QOV; -.
DR   PDBsum; 2QOX; -.
DR   PDBsum; 2QOZ; -.
DR   PDBsum; 2QP1; -.
DR   PDBsum; 2RDO; -.
DR   PDBsum; 2VHM; -.
DR   PDBsum; 2VHN; -.
DR   PDBsum; 2WWQ; -.
DR   PDBsum; 2Z4L; -.
DR   PDBsum; 2Z4N; -.
DR   PDBsum; 3BBX; -.
DR   PDBsum; 3DF2; -.
DR   PDBsum; 3DF4; -.
DR   PDBsum; 3E1B; -.
DR   PDBsum; 3E1D; -.
DR   PDBsum; 3FIK; -.
DR   PDBsum; 3I1N; -.
DR   PDBsum; 3I1P; -.
DR   PDBsum; 3I1R; -.
DR   PDBsum; 3I1T; -.
DR   PDBsum; 3I20; -.
DR   PDBsum; 3I22; -.
DR   PDBsum; 3IZT; -.
DR   PDBsum; 3IZU; -.
DR   PDBsum; 3J01; -.
DR   PDBsum; 3J0T; -.
DR   PDBsum; 3J0W; -.
DR   PDBsum; 3J0Y; -.
DR   PDBsum; 3J11; -.
DR   PDBsum; 3J12; -.
DR   PDBsum; 3J14; -.
DR   PDBsum; 3J19; -.
DR   PDBsum; 3J37; -.
DR   PDBsum; 3J4X; -.
DR   PDBsum; 3J50; -.
DR   PDBsum; 3J51; -.
DR   PDBsum; 3J52; -.
DR   PDBsum; 3J54; -.
DR   PDBsum; 3J56; -.
DR   PDBsum; 3J58; -.
DR   PDBsum; 3J5A; -.
DR   PDBsum; 3J5C; -.
DR   PDBsum; 3J5E; -.
DR   PDBsum; 3J5G; -.
DR   PDBsum; 3J5I; -.
DR   PDBsum; 3J5K; -.
DR   PDBsum; 3J5L; -.
DR   PDBsum; 3J5O; -.
DR   PDBsum; 3J5U; -.
DR   PDBsum; 3J5W; -.
DR   PDBsum; 3KCR; -.
DR   PDBsum; 3OAS; -.
DR   PDBsum; 3OAT; -.
DR   PDBsum; 3OFC; -.
DR   PDBsum; 3OFD; -.
DR   PDBsum; 3OFQ; -.
DR   PDBsum; 3OFR; -.
DR   PDBsum; 3OFZ; -.
DR   PDBsum; 3OG0; -.
DR   PDBsum; 3ORB; -.
DR   PDBsum; 3R8S; -.
DR   PDBsum; 3R8T; -.
DR   PDBsum; 3SGF; -.
DR   PDBsum; 3UOS; -.
DR   PDBsum; 4CSU; -.
DR   PDBsum; 4GAR; -.
DR   PDBsum; 4GAU; -.
DR   PDBsum; 4KIX; -.
DR   PDBsum; 4KIZ; -.
DR   PDBsum; 4KJ1; -.
DR   PDBsum; 4KJ3; -.
DR   PDBsum; 4KJ5; -.
DR   PDBsum; 4KJ7; -.
DR   PDBsum; 4KJ9; -.
DR   PDBsum; 4KJB; -.
DR   ProteinModelPortal; P0A7K6; -.
DR   SMR; P0A7K6; 2-115.
DR   DIP; DIP-35793N; -.
DR   IntAct; P0A7K6; 101.
DR   MINT; MINT-1245288; -.
DR   STRING; 511145.b2606; -.
DR   ChEMBL; CHEMBL2363135; -.
DR   PhosSite; P0810432; -.
DR   PaxDb; P0A7K6; -.
DR   PRIDE; P0A7K6; -.
DR   EnsemblBacteria; AAC75655; AAC75655; b2606.
DR   EnsemblBacteria; BAA16491; BAA16491; BAA16491.
DR   GeneID; 12931612; -.
DR   GeneID; 947096; -.
DR   KEGG; ecj:Y75_p2554; -.
DR   KEGG; eco:b2606; -.
DR   PATRIC; 32120611; VBIEscCol129921_2704.
DR   EchoBASE; EB0873; -.
DR   EcoGene; EG10880; rplS.
DR   eggNOG; COG0335; -.
DR   HOGENOM; HOG000016264; -.
DR   KO; K02884; -.
DR   OMA; GGRERIQ; -.
DR   OrthoDB; EOG6DZF5W; -.
DR   PhylomeDB; P0A7K6; -.
DR   BioCyc; EcoCyc:EG10880-MONOMER; -.
DR   BioCyc; ECOL316407:JW2587-MONOMER; -.
DR   EvolutionaryTrace; P0A7K6; -.
DR   PRO; PR:P0A7K6; -.
DR   Genevestigator; P0A7K6; -.
DR   GO; GO:0022625; C:cytosolic large ribosomal subunit; IDA:EcoCyc.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-HAMAP.
DR   HAMAP; MF_00402; Ribosomal_L19; 1.
DR   InterPro; IPR001857; Ribosomal_L19.
DR   InterPro; IPR018257; Ribosomal_L19_CS.
DR   InterPro; IPR008991; Translation_prot_SH3-like.
DR   PANTHER; PTHR15680; PTHR15680; 1.
DR   Pfam; PF01245; Ribosomal_L19; 1.
DR   PIRSF; PIRSF002191; Ribosomal_L19; 1.
DR   PRINTS; PR00061; RIBOSOMALL19.
DR   SUPFAM; SSF50104; SSF50104; 1.
DR   TIGRFAMs; TIGR01024; rplS_bact; 1.
DR   PROSITE; PS01015; RIBOSOMAL_L19; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Direct protein sequencing;
KW   Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW   rRNA-binding.
FT   INIT_MET      1      1       Removed. {ECO:0000269|PubMed:339951}.
FT   CHAIN         2    115       50S ribosomal protein L19.
FT                                /FTId=PRO_0000163451.
FT   HELIX         4     10
FT   STRAND       27     33
FT   TURN         34     36
FT   STRAND       38     44
FT   STRAND       46     50
FT   HELIX        54     56
FT   STRAND       58     62
FT   STRAND       66     68
FT   STRAND       70     75
FT   STRAND       76     78
FT   HELIX        79     81
FT   STRAND       83     85
FT   STRAND       86     88
FT   STRAND       93     95
FT   HELIX        98    102
FT   HELIX       105    107
SQ   SEQUENCE   115 AA;  13133 MW;  7BD9F2EFB9DDB06B CRC64;
     MSNIIKQLEQ EQMKQDVPSF RPGDTVEVKV WVVEGSKKRL QAFEGVVIAI RNRGLHSAFT
     VRKISNGEGV ERVFQTHSPV VDSISVKRRG AVRKAKLYYL RERTGKAARI KERLN
//
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