ID MKAR_CRYNB Reviewed; 361 AA.
AC P0CR35; Q55NM3; Q5KC11;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 29-MAY-2013, entry version 13.
DE RecName: Full=Very-long-chain 3-oxoacyl-CoA reductase;
DE EC=1.1.1.330;
DE AltName: Full=3-ketoacyl-CoA reductase;
DE Short=3-ketoreductase;
DE Short=KAR;
DE AltName: Full=Microsomal beta-keto-reductase;
GN OrderedLocusNames=CNBH0660;
OS Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS (Filobasidiella neoformans).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina;
OC Tremellomycetes; Tremellales; Tremellaceae; Filobasidiella;
OC Filobasidiella/Cryptococcus neoformans species complex.
OX NCBI_TaxID=283643;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B-3501A;
RX PubMed=15653466; DOI=10.1126/science.1103773;
RA Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J.,
RA D'Souza C.A., Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J.,
RA Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I.,
RA Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E.,
RA Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L.,
RA Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A.,
RA Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R.,
RA Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W.,
RA Fraser C.M., Hyman R.W.;
RT "The genome of the basidiomycetous yeast and human pathogen
RT Cryptococcus neoformans.";
RL Science 307:1321-1324(2005).
CC -!- FUNCTION: Component of the microsomal membrane bound fatty acid
CC elongation system, which produces the 26-carbon very long-chain
CC fatty acids (VLCFA) from palmitate. Catalyzes the reduction of the
CC 3-ketoacyl-CoA intermediate that is formed in each cycle of fatty
CC acid elongation. VLCFAs serve as precursors for ceramide and
CC sphingolipids (By similarity).
CC -!- CATALYTIC ACTIVITY: A very-long-chain (3R)-3-hydroxyacyl-CoA +
CC NADP(+) = a very-long-chain 3-oxoacyl-CoA + NADPH.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane (By
CC similarity); Single-pass membrane protein (Potential).
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC (SDR) family.
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DR EMBL; AAEY01000041; EAL19372.1; -; Genomic_DNA.
DR RefSeq; XP_774019.1; XM_768926.1.
DR ProteinModelPortal; P0CR35; -.
DR GeneID; 4937584; -.
DR KEGG; cnb:CNBH0660; -.
DR KO; K10251; -.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0045703; F:ketoreductase activity; IEA:InterPro.
DR GO; GO:0030497; P:fatty acid elongation; IEA:InterPro.
DR Gene3D; 3.40.50.720; -; 1.
DR HAMAP; MF_03107; 3-ketoreductase; 1; -.
DR InterPro; IPR027533; 3_ketoreductase_fungal.
DR InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR InterPro; IPR002347; Glc/ribitol_DH.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR Pfam; PF00106; adh_short; 1.
DR PIRSF; PIRSF000126; 11-beta-HSD1; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 3: Inferred from homology;
KW Complete proteome; Endoplasmic reticulum; Fatty acid biosynthesis;
KW Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism; Membrane;
KW NADP; Oxidoreductase; Transmembrane; Transmembrane helix.
FT CHAIN 1 361 Very-long-chain 3-oxoacyl-CoA reductase.
FT /FTId=PRO_0000410279.
FT TRANSMEM 32 52 Helical; (Potential).
FT ACT_SITE 236 236 Proton acceptor (By similarity).
FT BINDING 223 223 Substrate (By similarity).
SQ SEQUENCE 361 AA; 39197 MW; 4397C7DD0082B61A CRC64;
MVADTVHVGQ HLAGHPSVHL FGHEIVLDVS IPALILSTVG AAFLLRYTLS IFRLFLELTV
LPGKDIKSFQ SRKGETWAVV TGCTSGIGLE FARQLAAKKF NIILVGRRQS ALTDLSKEIE
SKYDVHTKSV TVDVSTPGSA RDDALTQLEL LAQNLDVGIL INNVGASHSM PVAFHETERS
EMSRIIETNV TWTYLVTRSI LPSMVARSKQ KGAPKSLVIT IGSLSGRIPS PLLASYSGTK
AALATWTKAL AEEVKPQGVI VELVQAAFVV SNMSKIRKSS PFVPTPAPFV RSTLNSIGLP
RGAQGRPHER TPFWSHAILD YVVGFAGYVS EMAGIKVILG MHKDIRKRAL KKAARDEKKA
E
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