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Database: UniProt
Entry: P0CR35
LinkDB: P0CR35
Original site: P0CR35 
ID   MKAR_CRYNB              Reviewed;         361 AA.
AC   P0CR35; Q55NM3; Q5KC11;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   19-FEB-2014, entry version 18.
DE   RecName: Full=Very-long-chain 3-oxoacyl-CoA reductase;
DE            EC=1.1.1.330;
DE   AltName: Full=3-ketoacyl-CoA reductase;
DE            Short=3-ketoreductase;
DE            Short=KAR;
DE   AltName: Full=Microsomal beta-keto-reductase;
GN   OrderedLocusNames=CNBH0660;
OS   Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS   (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina;
OC   Tremellomycetes; Tremellales; Tremellaceae; Filobasidiella;
OC   Filobasidiella/Cryptococcus neoformans species complex.
OX   NCBI_TaxID=283643;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B-3501A;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J.,
RA   D'Souza C.A., Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J.,
RA   Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I.,
RA   Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E.,
RA   Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L.,
RA   Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A.,
RA   Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R.,
RA   Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W.,
RA   Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen
RT   Cryptococcus neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Component of the microsomal membrane bound fatty acid
CC       elongation system, which produces the 26-carbon very long-chain
CC       fatty acids (VLCFA) from palmitate. Catalyzes the reduction of the
CC       3-ketoacyl-CoA intermediate that is formed in each cycle of fatty
CC       acid elongation. VLCFAs serve as precursors for ceramide and
CC       sphingolipids (By similarity).
CC   -!- CATALYTIC ACTIVITY: A very-long-chain (3R)-3-hydroxyacyl-CoA +
CC       NADP(+) = a very-long-chain 3-oxoacyl-CoA + NADPH.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane (By
CC       similarity); Single-pass membrane protein (Potential).
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family.
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DR   EMBL; AAEY01000041; EAL19372.1; -; Genomic_DNA.
DR   RefSeq; XP_774019.1; XM_768926.1.
DR   ProteinModelPortal; P0CR35; -.
DR   GeneID; 4937584; -.
DR   KEGG; cnb:CNBH0660; -.
DR   KO; K10251; -.
DR   OrthoDB; EOG7NPG46; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0045703; F:ketoreductase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0030497; P:fatty acid elongation; IEA:UniProtKB-HAMAP.
DR   Gene3D; 3.40.50.720; -; 1.
DR   HAMAP; MF_03107; 3_ketoreductase; 1.
DR   InterPro; IPR027533; 3_ketoreductase_fungal.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR002347; Glc/ribitol_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   Pfam; PF00106; adh_short; 1.
DR   PIRSF; PIRSF000126; 11-beta-HSD1; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Endoplasmic reticulum; Fatty acid biosynthesis;
KW   Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism; Membrane;
KW   NADP; Oxidoreductase; Transmembrane; Transmembrane helix.
FT   CHAIN         1    361       Very-long-chain 3-oxoacyl-CoA reductase.
FT                                /FTId=PRO_0000410279.
FT   TRANSMEM     32     52       Helical; (Potential).
FT   ACT_SITE    236    236       Proton acceptor (By similarity).
FT   BINDING     223    223       Substrate (By similarity).
SQ   SEQUENCE   361 AA;  39197 MW;  4397C7DD0082B61A CRC64;
     MVADTVHVGQ HLAGHPSVHL FGHEIVLDVS IPALILSTVG AAFLLRYTLS IFRLFLELTV
     LPGKDIKSFQ SRKGETWAVV TGCTSGIGLE FARQLAAKKF NIILVGRRQS ALTDLSKEIE
     SKYDVHTKSV TVDVSTPGSA RDDALTQLEL LAQNLDVGIL INNVGASHSM PVAFHETERS
     EMSRIIETNV TWTYLVTRSI LPSMVARSKQ KGAPKSLVIT IGSLSGRIPS PLLASYSGTK
     AALATWTKAL AEEVKPQGVI VELVQAAFVV SNMSKIRKSS PFVPTPAPFV RSTLNSIGLP
     RGAQGRPHER TPFWSHAILD YVVGFAGYVS EMAGIKVILG MHKDIRKRAL KKAARDEKKA
     E
//
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