ID MBHL_BRAJA Reviewed; 596 AA.
AC P12636;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 1.
DT 01-MAY-2013, entry version 89.
DE RecName: Full=Uptake hydrogenase large subunit;
DE EC=1.12.99.6;
DE AltName: Full=Hydrogenlyase;
DE AltName: Full=Membrane-bound hydrogenase large subunit;
GN Name=hupB; Synonyms=hupL; OrderedLocusNames=bll6941;
OS Bradyrhizobium japonicum (strain USDA 110).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC Bradyrhizobiaceae; Bradyrhizobium.
OX NCBI_TaxID=224911;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3054886; DOI=10.1073/pnas.85.22.8395;
RA Sayavedra-Soto L.A., Powell G.K., Evans H.J., Morris R.O.;
RT "Nucleotide sequence of the genetic loci encoding subunits of
RT Bradyrhizobium japonicum uptake hydrogenase.";
RL Proc. Natl. Acad. Sci. U.S.A. 85:8395-8399(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=USDA 110;
RX PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T.,
RA Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K.,
RA Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M.,
RA Tabata S.;
RT "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT Bradyrhizobium japonicum USDA110.";
RL DNA Res. 9:189-197(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 589-596.
RX PubMed=8163174; DOI=10.1016/0378-1119(94)90126-0;
RA Fu C., Maier R.J.;
RT "Sequence and characterization of three genes within the hydrogenase
RT gene cluster of Bradyrhizobium japonicum.";
RL Gene 141:47-52(1994).
CC -!- FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to
CC increase the production of ATP and to protect nitrogenase against
CC inhibition or damage by O(2) under carbon- or phosphate-limited
CC conditions.
CC -!- CATALYTIC ACTIVITY: H(2) + A = AH(2).
CC -!- COFACTOR: Binds 1 nickel ion per subunit (By similarity).
CC -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large
CC subunit family.
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DR EMBL; J04114; AAA26219.1; -; Genomic_DNA.
DR EMBL; BA000040; BAC52206.1; -; Genomic_DNA.
DR EMBL; L24446; AAD13471.1; -; Genomic_DNA.
DR PIR; B31341; HQZJUL.
DR RefSeq; NP_773581.1; NC_004463.1.
DR ProteinModelPortal; P12636; -.
DR STRING; 224911.bll6941; -.
DR EnsemblBacteria; BAC52206; BAC52206; BAC52206.
DR GeneID; 1048593; -.
DR KEGG; bja:bll6941; -.
DR PATRIC; 21197757; VBIBraJap65052_7112.
DR eggNOG; COG0374; -.
DR HOGENOM; HOG000278799; -.
DR KO; K06281; -.
DR OMA; HLRVEVN; -.
DR ProtClustDB; CLSK820329; -.
DR BioCyc; BJAP224911:GJEJ-6993-MONOMER; -.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR GO; GO:0033748; F:hydrogenase (acceptor) activity; IEA:EC.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR InterPro; IPR001501; Ni-dep_hyd_lsu.
DR InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR Pfam; PF00374; NiFeSe_Hases; 1.
DR PROSITE; PS00507; NI_HGENASE_L_1; 1.
DR PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE 3: Inferred from homology;
KW Cell membrane; Complete proteome; Membrane; Metal-binding; Nickel;
KW Oxidoreductase; Reference proteome.
FT CHAIN 1 596 Uptake hydrogenase large subunit.
FT /FTId=PRO_0000199710.
FT METAL 75 75 Nickel (Potential).
FT METAL 78 78 Nickel (Potential).
FT METAL 575 575 Nickel (Potential).
FT METAL 578 578 Nickel (Potential).
SQ SEQUENCE 596 AA; 65923 MW; 0409EE8972F036B7 CRC64;
MGIQTPNGFN LDNSGKRIVV DPVTRIEGHM RVEVNVDADN VIRNAVSTGT MWRGIEVILK
NRDPRDAWAF TERICGVCTG THALTSVRAV ENALGITIPE NANSIRNLMQ LALQVHDHVV
HFYHLHALDW VDVVSALSAD PRATSTLAQS ISNWPLSSPG YFKDLQTRLK KFVESGQLGP
FKNGYWGSKA YKLPPEANLM AVAHYLEALD FQKEIVKIHT IFGGKNPHPN WLVGGVPCPI
NVDGTGAVGA INMERLNLIS SIIDRLIEFN EMVYLPDVAA IGSFYKDWLY GGGLSGQSVL
AYGDVPEHAN DYSAKSLKLP RGAIINGNLS EVFPVDHANP DEIQEFVVHS WYKYPDETKG
LHPWDGVTEP NYVLGPNAKG TKTAIEQLDE GGKYSWIKAP RWKGHAMEVG PLARWVVGYA
QNKSEFKDPV DKFLRDLNLP TSALFSTLGR TAARALESVW AGRQMRYFQD KLVANIKAGD
SSTANVDKWK PESWPKEAKG VGFTEAPRGA LAHWIKIKDT KIDNYQCVVP TTWNGSPRDP
KGNIGAFEAS LMNTPMVNPE QPLEILRTIH SFDPCLACST HVMSPDGQEL AKVKVR
//
