ID UNC86_CAEEL Reviewed; 357 AA.
AC P13528; D7SFI2;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 12-OCT-2022, sequence version 2.
DT 27-MAR-2024, entry version 182.
DE RecName: Full=Transcription factor unc-86;
DE AltName: Full=Uncoordinated protein 86;
GN Name=unc-86; ORFNames=C30A5.7;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS A AND B).
RX PubMed=2903797; DOI=10.1016/0092-8674(88)90132-8;
RA Finney M., Ruvkun G., Horvitz H.R.;
RT "The C. elegans cell lineage and differentiation gene unc-86 encodes a
RT protein with a homeodomain and extended similarity to transcription
RT factors.";
RL Cell 55:757-769(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=Bristol N2;
RX PubMed=7237544; DOI=10.1016/0092-8674(81)90501-8;
RA Chalfie M., Horvitz H.R., Sulston J.E.;
RT "Mutations that lead to reiterations in the cell lineages of C. elegans.";
RL Cell 24:59-69(1981).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=2257628; DOI=10.1016/0092-8674(90)90493-x;
RA Finney M., Ruvkun G.;
RT "The unc-86 gene product couples cell lineage and cell identity in C.
RT elegans.";
RL Cell 63:895-905(1990).
RN [6]
RP FUNCTION.
RX PubMed=1361171; DOI=10.1002/j.1460-2075.1992.tb05604.x;
RA Xue D., Finney M., Ruvkun G., Chalfie M.;
RT "Regulation of the mec-3 gene by the C.elegans homeoproteins UNC-86 and
RT MEC-3.";
RL EMBO J. 11:4969-4979(1992).
RN [7]
RP FUNCTION, AND SUBUNIT.
RC STRAIN=Bristol N2;
RX PubMed=9735371; DOI=10.1242/dev.125.20.4107;
RA Duggan A., Ma C., Chalfie M.;
RT "Regulation of touch receptor differentiation by the Caenorhabditis elegans
RT mec-3 and unc-86 genes.";
RL Development 125:4107-4119(1998).
RN [8]
RP FUNCTION, SUBUNIT, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF PRO-160 AND
RP LEU-210.
RX PubMed=10899123; DOI=10.1093/emboj/19.14.3694;
RA Rohrig S., Rockelein I., Donhauser R., Baumeister R.;
RT "Protein interaction surface of the POU transcription factor UNC-86
RT selectively used in touch neurons.";
RL EMBO J. 19:3694-3703(2000).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=Bristol N2;
RX PubMed=12135927; DOI=10.1242/dev.129.16.3901;
RA Sze J.Y., Zhang S., Li J., Ruvkun G.;
RT "The C. elegans POU-domain transcription factor UNC-86 regulates the tph-1
RT tryptophan hydroxylase gene and neurite outgrowth in specific serotonergic
RT neurons.";
RL Development 129:3901-3911(2002).
RN [10]
RP FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Bristol N2;
RX PubMed=11959845; DOI=10.1101/gad.976002;
RA Shaham S., Bargmann C.I.;
RT "Control of neuronal subtype identity by the C. elegans ARID protein CFI-
RT 1.";
RL Genes Dev. 16:972-983(2002).
RN [11]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=12883006; DOI=10.1073/pnas.1530752100;
RA Sze J.Y., Ruvkun G.;
RT "Activity of the Caenorhabditis elegans UNC-86 POU transcription factor
RT modulates olfactory sensitivity.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:9560-9565(2003).
RN [12]
RP FUNCTION.
RC STRAIN=Bristol N2;
RX PubMed=16139210; DOI=10.1016/j.cub.2005.07.057;
RA Kage E., Hayashi Y., Takeuchi H., Hirotsu T., Kunitomo H., Inoue T.,
RA Arai H., Iino Y., Kubo T.;
RT "MBR-1, a novel helix-turn-helix transcription factor, is required for
RT pruning excessive neurites in Caenorhabditis elegans.";
RL Curr. Biol. 15:1554-1559(2005).
RN [13]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=Bristol N2;
RX PubMed=21656875; DOI=10.1002/dvdy.22667;
RA Olsson-Carter K., Slack F.J.;
RT "The POU transcription factor UNC-86 controls the timing and ventral
RT guidance of Caenorhabditis elegans axon growth.";
RL Dev. Dyn. 240:1815-1825(2011).
RN [14]
RP FUNCTION.
RC STRAIN=Bristol N2;
RX PubMed=23932402; DOI=10.1016/j.cub.2013.06.058;
RA Schroeder N.E., Androwski R.J., Rashid A., Lee H., Lee J., Barr M.M.;
RT "Dauer-specific dendrite arborization in C. elegans is regulated by KPC-
RT 1/Furin.";
RL Curr. Biol. 23:1527-1535(2013).
RN [15]
RP FUNCTION, DNA-BINDING, AND DISRUPTION PHENOTYPE.
RX PubMed=24353061; DOI=10.1242/dev.099721;
RA Zhang F., Bhattacharya A., Nelson J.C., Abe N., Gordon P.,
RA Lloret-Fernandez C., Maicas M., Flames N., Mann R.S., Colon-Ramos D.A.,
RA Hobert O.;
RT "The LIM and POU homeobox genes ttx-3 and unc-86 act as terminal selectors
RT in distinct cholinergic and serotonergic neuron types.";
RL Development 141:422-435(2014).
RN [16]
RP FUNCTION, AND MUTAGENESIS OF LEU-210.
RX PubMed=26096732; DOI=10.1016/j.devcel.2015.04.023;
RA Gordon P.M., Hobert O.;
RT "A competition mechanism for a homeotic neuron identity transformation in
RT C. elegans.";
RL Dev. Cell 34:206-219(2015).
CC -!- FUNCTION: Transcription factor required for correct cell fate
CC determination and differentiation in diverse neuronal cell lineages
CC where it plays a role in specifying the fate of daughter cells during
CC cell divisions (PubMed:7237544, PubMed:2257628, PubMed:26096732,
CC PubMed:10899123). Involved in sensory neuron production and function
CC (PubMed:1361171, PubMed:9735371, PubMed:10899123, PubMed:26096732).
CC Binds both alone and with mec-3 to the mec-3 promoter to initiate and
CC maintain mec-3 expression which is required for sensory neuron
CC differentiation (PubMed:1361171, PubMed:9735371). In addition, binds
CC both alone and with mec-3 to the promoters of mec-4 and mec-7 which act
CC to regulate sensory neuron function (PubMed:9735371, PubMed:10899123).
CC Involved in determining the identity of the serotonergic NSM neurons
CC and the cholinergic IL2 sensory and URA motor neurons
CC (PubMed:24353061). Promotes expression of the cfi-1 transcription
CC factor in the URA and IL2 neurons which in turn activates normal URA
CC and IL2 gene expression (PubMed:24353061, PubMed:11959845). Required to
CC determine the identity of BDU sensory neurons in concert with
CC transcription factor unc-86, regulating expression of a number of
CC genes, including transcription factors ceh-14 and ahr-1, neuropeptides
CC flp-10, nlp-1 and nlp-15, and tyramine receptor-encoding ser-2
CC (PubMed:26096732). Regulates expression of a number of genes in NSM
CC neurons including bas-1, cat-1, dop-3, mgl-3, nlp-13, scd-2 and ptps-1
CC (PubMed:24353061). In the IL2 neurons, required for expression of cho-
CC 1, gcy-19, klp-6, lag-2, unc-5 and unc-17 (PubMed:24353061). Promotes
CC expression of pkd-2 in the male-specific CEM head neurons
CC (PubMed:11959845). Required for dauer-specific branching of IL2Q
CC neurons and nictation behavior (PubMed:23932402). Controls both the
CC timing and direction of axon outgrowth in HSN neurons
CC (PubMed:21656875). Plays a role in serotonin production by regulating
CC expression of the tryptophan hydrolase tph-1 which catalyzes serotonin
CC synthesis, in the AIM, NSM, HSN and RIH neurons (PubMed:12135927).
CC Involved in regulation of lin-11 expression in the AIZ interneurons,
CC the major interneurons of the olfactory pathway, and is required for
CC odortaxis behavior (PubMed:12883006). Involved in neurite pruning
CC between AIM neurons during larval development by regulating the
CC expression of transcription factor mbr-1 (PubMed:16139210). Required
CC for correct localization of unc-40 (PubMed:24353061).
CC {ECO:0000269|PubMed:10899123, ECO:0000269|PubMed:11959845,
CC ECO:0000269|PubMed:12135927, ECO:0000269|PubMed:12883006,
CC ECO:0000269|PubMed:1361171, ECO:0000269|PubMed:16139210,
CC ECO:0000269|PubMed:21656875, ECO:0000269|PubMed:2257628,
CC ECO:0000269|PubMed:23932402, ECO:0000269|PubMed:24353061,
CC ECO:0000269|PubMed:7237544, ECO:0000269|PubMed:9735371}.
CC -!- SUBUNIT: Interacts with mec-3; the heterooligomer binds to the
CC promoters of mec-3, mec-4 and mec-7. {ECO:0000269|PubMed:10899123,
CC ECO:0000269|PubMed:9735371}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000255|PROSITE-ProRule:PRU00530, ECO:0000269|PubMed:2257628}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b;
CC IsoId=P13528-1; Sequence=Displayed;
CC Name=a;
CC IsoId=P13528-2; Sequence=VSP_002344;
CC -!- TISSUE SPECIFICITY: Specific to neurons and neuroblasts. Expressed in
CC CEM head neurons and in IL2, URA, URB, URX and URY neurons. Not
CC expressed in olfactory sensory neurons but expressed in AIZ
CC interneurons. {ECO:0000269|PubMed:11959845,
CC ECO:0000269|PubMed:12883006, ECO:0000269|PubMed:2257628}.
CC -!- DEVELOPMENTAL STAGE: First appears during embryogenesis and persists
CC throughout adulthood. {ECO:0000269|PubMed:2257628}.
CC -!- DISRUPTION PHENOTYPE: In a number of neuronal cell lineages, one of the
CC two daughter cells of a division fails to assume its normal fate,
CC retaining instead the fate of its parent cell. Altered gene expression
CC in a number of neuron types including reduced cat-1 expression in AIM,
CC NSM, HSN and RIH neurons, loss of cat-4 expression in HSN neurons, loss
CC of expression of dop-3 and mgl-3 and reduced expression of bas-1, nlp-
CC 13, ptpa-1 and scd-2 in NSM neurons, loss of expression of gcy-19, klp-
CC 6, lag-2 and unc-17 and reduced expression of cho-1 and unc-5 in IL2
CC neurons, and loss of tph-1 expression in AIM, NSM, HSN and RIH neurons
CC which leads to defects in serotonin production. Defective motility
CC along with defects in IL2Q dendritic arborization and nictation.
CC Abnormal neurite outgrowth in NSM neurons. Defective response to odor
CC attractants but odor repellants are avoided normally. Penetrant ventral
CC axon growth defects in HSN motor neurons with extension of processes in
CC L1, three stages earlier than wild-type. Incorrect unc-40 localization.
CC Defective egg-laying characterized by retention of substantially more
CC eggs in the uterus of second-day animals than the wild-type.
CC {ECO:0000269|PubMed:10899123, ECO:0000269|PubMed:12135927,
CC ECO:0000269|PubMed:12883006, ECO:0000269|PubMed:21656875,
CC ECO:0000269|PubMed:24353061, ECO:0000269|PubMed:7237544}.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-4
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA28158.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAA28159.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; M22363; AAA28158.1; ALT_SEQ; Genomic_DNA.
DR EMBL; M22363; AAA28159.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BX284603; CCD62655.2; -; Genomic_DNA.
DR EMBL; BX284603; CCD62656.1; -; Genomic_DNA.
DR PIR; A30042; A30042.
DR PIR; S44778; S44778.
DR RefSeq; NP_001021191.2; NM_001026020.4. [P13528-1]
DR RefSeq; NP_001309473.1; NM_001322687.1. [P13528-2]
DR AlphaFoldDB; P13528; -.
DR SMR; P13528; -.
DR BioGRID; 41362; 2.
DR IntAct; P13528; 1.
DR STRING; 6239.C30A5.7b.1; -.
DR EPD; P13528; -.
DR PaxDb; 6239-C30A5-7b; -.
DR EnsemblMetazoa; C30A5.7a.1; C30A5.7a.1; WBGene00006818. [P13528-2]
DR EnsemblMetazoa; C30A5.7b.1; C30A5.7b.1; WBGene00006818. [P13528-1]
DR GeneID; 176157; -.
DR KEGG; cel:CELE_C30A5.7; -.
DR UCSC; C30A5.7b; c. elegans. [P13528-1]
DR AGR; WB:WBGene00006818; -.
DR WormBase; C30A5.7a; CE51471; WBGene00006818; unc-86.
DR WormBase; C30A5.7b; CE45067; WBGene00006818; unc-86.
DR eggNOG; KOG1168; Eukaryota.
DR GeneTree; ENSGT00940000169264; -.
DR InParanoid; P13528; -.
DR OMA; NPLMRPH; -.
DR OrthoDB; 4250502at2759; -.
DR PhylomeDB; P13528; -.
DR SignaLink; P13528; -.
DR PRO; PR:P13528; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00006818; Expressed in pharyngeal muscle cell (C elegans) and 3 other cell types or tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:WormBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IPI:WormBase.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0007638; P:mechanosensory behavior; IMP:WormBase.
DR GO; GO:0048666; P:neuron development; IMP:WormBase.
DR GO; GO:0048665; P:neuron fate specification; IMP:WormBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0009612; P:response to mechanical stimulus; IMP:WormBase.
DR GO; GO:0007608; P:sensory perception of smell; IEA:UniProtKB-KW.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR Gene3D; 1.10.260.40; lambda repressor-like DNA-binding domains; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR PANTHER; PTHR11636:SF70; INHIBITORY POU PROTEIN; 1.
DR PANTHER; PTHR11636; POU DOMAIN; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR SUPFAM; SSF47413; lambda repressor-like DNA-binding domains; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Homeobox; Neurogenesis; Nucleus;
KW Olfaction; Reference proteome; Sensory transduction; Transcription;
KW Transcription regulation.
FT CHAIN 1..357
FT /note="Transcription factor unc-86"
FT /id="PRO_0000100780"
FT DOMAIN 155..232
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 253..312
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT MOTIF 35..44
FT /note="POU-IV box"
FT VAR_SEQ 1..56
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_002344"
FT MUTAGEN 160
FT /note="P->S: In u168; defective mechanosensation, lack of
FT interaction with mec-3 and lack of maintained mec-3 gene
FT expression. No egg-laying or Q neuroblast lineage defects."
FT /evidence="ECO:0000269|PubMed:10899123"
FT MUTAGEN 210
FT /note="L->F: In u5; defective mechanosensation, lack of
FT interaction with mec-3 and lack of maintained mec-3 gene
FT expression. No egg-laying or Q neuroblast lineage defects.
FT Abolishes expression of ALM neuron-specific genes mec-17
FT and mec-4. Abnormal, ectopic expression of BDU-specific
FT genes ceh-14, flp-10 and zig-3 in ALM."
FT /evidence="ECO:0000269|PubMed:10899123,
FT ECO:0000269|PubMed:26096732"
SQ SEQUENCE 357 AA; 39737 MW; 1B9830CA8E04ADAC CRC64;
MQNTAPVPTT TTASKMQPFN NSLFGSFDDP ILNARAAQVA LADIDVKNVP QLTNPLMRPH
DMFSYSNYFS GIHDTSAATN IYQGLPSSSE PFDASVVVPT SSDDQMTPLQ QVMAMQQSYG
APPPFQYNMT HPFSTTSIAS SNNLARYPIA PPTSDMDTDP RQLETFAEHF KQRRIKLGVT
QADVGKALAH LKMPGVGSLS QSTICRFESL TLSHNNMVAL KPILHSWLEK AEEAMKQKDT
IGDINGILPN TDKKRKRTSI AAPEKRELEQ FFKQQPRPSG ERIASIADRL DLKKNVVRVW
FCNQRQKQKR DFRSQFRARS AAAVMGPRVM PVLNGNNSNN NLKQGQTTYN GLPGFFD
//
