ID RAB7A_CANFA Reviewed; 207 AA.
AC P18067;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 1.
DT 01-MAY-2013, entry version 112.
DE RecName: Full=Ras-related protein Rab-7a;
GN Name=RAB7A; Synonyms=RAB7;
OS Canis familiaris (Dog) (Canis lupus familiaris).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae;
OC Canis.
OX NCBI_TaxID=9615;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2115402; DOI=10.1016/0092-8674(90)90369-P;
RA Chavrier P., Parton R.G., Hauri H.P., Simons K., Zerial M.;
RT "Localization of low molecular weight GTP binding proteins to exocytic
RT and endocytic compartments.";
RL Cell 62:317-329(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Cocker spaniel; TISSUE=Kidney;
RX PubMed=2123294;
RA Chavrier P., Vingron M., Sander C., Simons K., Zerial M.;
RT "Molecular cloning of YPT1/SEC4-related cDNAs from an epithelial cell
RT line.";
RL Mol. Cell. Biol. 10:6578-6585(1990).
RN [3]
RP FUNCTION.
RX PubMed=8522602; DOI=10.1083/jcb.131.6.1435;
RA Feng Y., Press B., Wandinger-Ness A.;
RT "Rab 7: an important regulator of late endocytic membrane traffic.";
RL J. Cell Biol. 131:1435-1452(1995).
RN [4]
RP FUNCTION.
RX PubMed=9490721; DOI=10.1083/jcb.140.5.1075;
RA Press B., Feng Y., Hoflack B., Wandinger-Ness A.;
RT "Mutant Rab7 causes the accumulation of cathepsin D and cation-
RT independent mannose 6-phosphate receptor in an early endocytic
RT compartment.";
RL J. Cell Biol. 140:1075-1089(1998).
RN [5]
RP FUNCTION.
RX PubMed=16282324; DOI=10.1074/jbc.M504175200;
RA Ceresa B.P., Bahr S.J.;
RT "rab7 activity affects epidermal growth factor:epidermal growth factor
RT receptor degradation by regulating endocytic trafficking from the late
RT endosome.";
RL J. Biol. Chem. 281:1099-1106(2006).
CC -!- FUNCTION: Key regulator in endo-lysosomal trafficking. Governs
CC early-to-late endosomal maturation, microtubule minus-end as well
CC as plus-end directed endosomal migration and positioning, and
CC endosome-lysosome transport through different protein-protein
CC interaction cascades. Plays a central role, not only in endosomal
CC traffic, but also in many other cellular and physiological events,
CC such as growth-factor-mediated cell signaling, nutrient-
CC transportor mediated nutrient uptake, neurotrophin transport in
CC the axons of neurons and lipid metabolism. Also involved in
CC regulation of some specialized endosomal membrane trafficking,
CC such as maturation of melanosomes, pathogen-induced phagosomes (or
CC vacuoles) and autophagosomes. Plays a role in the maturation and
CC acidification of phagosomes that engulf pathogens, such as
CC S.aureus and Mycobacteria. Plays a role in the fusion of
CC phagosomes with lysosomes (By similarity). Plays important roles
CC in microbial pathogen infection and survival, as well as in
CC participating in the life cycle of viruses. Microbial pathogens
CC possess survival strategies governed by RAB7A, sometimes by
CC employing RAB7A function (e.g. Salmonella) and sometimes by
CC excluding RAB7A function (e.g. Mycobacterium). In concert with
CC RAC1, plays a role in regulating the formation of RBs (ruffled
CC borders) in osteoclasts. Controls the endosomal trafficking and
CC neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic
CC trafficking of the EGF-EGFR complex by regulating its lysosomal
CC degradation.
CC -!- SUBUNIT: Interacts with NTRK1/TRKA, RILP, PSMA7, RNF115 and FYCO1.
CC Interacts with the PIK3C3/VPS34-PIK3R4 complex. The GTP-bound form
CC interacts with OSBPL1A and RAC1 (By similarity). Interacts with
CC CLN3 (By similarity).
CC -!- SUBCELLULAR LOCATION: Late endosome. Lysosome (By similarity).
CC Cytoplasmic vesicle, phagosome (By similarity). Cytoplasmic
CC vesicle, phagosome membrane; Lipid-anchor; Cytoplasmic side (By
CC similarity). Melanosome (By similarity). Note=Co-localizes with
CC OSBPL1A at the late endosome. Found in the ruffled border (a late
CC endosomal-like compartment in the plasma membrane) of bone-
CC resorbing osteoclasts. Recruited to phagosomes containing S.aureus
CC or Mycobacterium (By similarity).
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
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DR EMBL; M35522; AAA30890.1; -; mRNA.
DR PIR; B30413; B30413.
DR RefSeq; NP_001003316.1; NM_001003316.1.
DR UniGene; Cfa.45048; -.
DR ProteinModelPortal; P18067; -.
DR SMR; P18067; 7-190.
DR STRING; 9615.ENSCAFP00000034211; -.
DR PaxDb; P18067; -.
DR PRIDE; P18067; -.
DR Ensembl; ENSCAFT00000038482; ENSCAFP00000034211; ENSCAFG00000004157.
DR GeneID; 404007; -.
DR KEGG; cfa:404007; -.
DR CTD; 7879; -.
DR eggNOG; COG1100; -.
DR GeneTree; ENSGT00700000104345; -.
DR HOGENOM; HOG000233968; -.
DR HOVERGEN; HBG009351; -.
DR InParanoid; P18067; -.
DR KO; K07897; -.
DR OMA; SPRDPEH; -.
DR OrthoDB; EOG4QFWF4; -.
DR BindingDB; P18067; -.
DR NextBio; 20817494; -.
DR GO; GO:0005794; C:Golgi apparatus; IEA:Compara.
DR GO; GO:0005770; C:late endosome; ISS:UniProtKB.
DR GO; GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
DR GO; GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
DR GO; GO:0045335; C:phagocytic vesicle; ISS:UniProtKB.
DR GO; GO:0030670; C:phagocytic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0019003; F:GDP binding; IEA:Compara.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:Compara.
DR GO; GO:0045022; P:early endosome to late endosome transport; IMP:UniProtKB.
DR GO; GO:0008333; P:endosome to lysosome transport; IEA:Compara.
DR GO; GO:0007174; P:epidermal growth factor catabolic process; IMP:UniProtKB.
DR GO; GO:0090383; P:phagosome acidification; ISS:UniProtKB.
DR GO; GO:0090385; P:phagosome-lysosome fusion; ISS:UniProtKB.
DR GO; GO:0006622; P:protein targeting to lysosome; IEA:Compara.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR003579; Small_GTPase_Rab_type.
DR Pfam; PF00071; Ras; 1.
DR PRINTS; PR00449; RASTRNSFRMNG.
DR SMART; SM00175; RAB; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 2: Evidence at transcript level;
KW Complete proteome; Cytoplasmic vesicle; Endosome; GTP-binding;
KW Lipoprotein; Lysosome; Membrane; Methylation; Nucleotide-binding;
KW Phosphoprotein; Prenylation; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1 207 Ras-related protein Rab-7a.
FT /FTId=PRO_0000121120.
FT NP_BIND 15 22 GTP (By similarity).
FT NP_BIND 63 67 GTP (By similarity).
FT NP_BIND 125 128 GTP (By similarity).
FT MOTIF 37 45 Effector region (By similarity).
FT MOD_RES 72 72 Phosphoserine (By similarity).
FT MOD_RES 207 207 Cysteine methyl ester (By similarity).
FT LIPID 205 205 S-geranylgeranyl cysteine (By
FT similarity).
FT LIPID 207 207 S-geranylgeranyl cysteine (By
FT similarity).
SQ SEQUENCE 207 AA; 23520 MW; E3AF33B16A67296D CRC64;
MTSRKKVLLK VIILGDSGVG KTSLMNQYVN KKFSNQYKAT IGADFLTKEV MVDDRLVTMQ
IWDTAGQERF QSLGVAFYRG ADCCVLVFDV TAPNTFKTLD SWRDEFLIQA SPRDPENFPF
VVLGNKIDLE NRQVATKRAQ AWCYSKNNIP YFETSAKEAI NVEQAFQTIA RNALKQETEV
ELYNEFPEPI KLDKNDRAKT SAESCSC
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