ID SPLA_DICDI Reviewed; 2410 AA.
AC P18160; Q54R51;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 3.
DT 01-MAY-2013, entry version 111.
DE RecName: Full=Dual specificity protein kinase splA;
DE EC=2.7.10.2;
DE AltName: Full=Non-receptor tyrosine kinase spore lysis A;
DE AltName: Full=Tyrosine-protein kinase 1;
GN Name=splA; Synonyms=DpyK1, pyK1, pykA; ORFNames=DDB_G0283385;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 827-2410, AUTOPHOSPHORYLATION,
RP AND DEVELOPMENTAL STAGE.
RC STRAIN=JH10;
RX PubMed=8898241;
RA Nuckolls G.H., Osherov N., Loomis W.F., Spudich J.A.;
RT "The Dictyostelium dual-specificity kinase splA is essential for spore
RT differentiation.";
RL Development 122:3295-3305(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 2074-2410.
RX PubMed=1972546;
RA Tan J.L., Spudich J.A.;
RT "Developmentally regulated protein-tyrosine kinase genes in
RT Dictyostelium discoideum.";
RL Mol. Cell. Biol. 10:3578-3583(1990).
CC -!- FUNCTION: Essential for spore differentiation.
CC -!- CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a
CC [protein]-L-tyrosine phosphate.
CC -!- DEVELOPMENTAL STAGE: Expressed in vegetative cells and throughout
CC development with a peak during the mound stage of morphogenesis.
CC -!- PTM: Tyrosine kinase domain is capable of autophosphorylation, in
CC vitro; however it is also autophosphorylated on serine and
CC threonine residues.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. TKL Tyr
CC protein kinase family.
CC -!- SIMILARITY: Contains 3 B30.2/SPRY domains.
CC -!- SIMILARITY: Contains 1 protein kinase domain.
CC -!- SIMILARITY: Contains 1 SAM (sterile alpha motif) domain.
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DR EMBL; AAFI02000055; EAL65677.1; -; Genomic_DNA.
DR EMBL; U32174; AAB41125.1; -; Genomic_DNA.
DR EMBL; M33785; AAA33202.1; -; mRNA.
DR PIR; T18276; T18276.
DR RefSeq; XP_639040.1; XM_633948.1.
DR ProteinModelPortal; P18160; -.
DR EnsemblProtists; DDB0252636; DDB0252636; DDB_G0283385.
DR GeneID; 8624065; -.
DR KEGG; ddi:DDB_G0283385; -.
DR dictyBase; DDB_G0283385; splA.
DR eggNOG; COG0515; -.
DR BRENDA; 2.7.12.1; 1939.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IEA:EC.
DR GO; GO:0004712; F:protein serine/threonine/tyrosine kinase activity; IDA:dictyBase.
DR GO; GO:0004713; F:protein tyrosine kinase activity; IDA:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IMP:dictyBase.
DR Gene3D; 1.10.150.50; -; 1.
DR InterPro; IPR001870; B30.2/SPRY.
DR InterPro; IPR008985; ConA-like_lec_gl_sf.
DR InterPro; IPR011009; Kinase-like_dom.
DR InterPro; IPR000719; Prot_kinase_cat_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR001660; SAM.
DR InterPro; IPR013761; SAM/pointed.
DR InterPro; IPR011510; SAM_2.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR InterPro; IPR018355; SPla/RYanodine_receptor_subgr.
DR InterPro; IPR003877; SPRY_rcpt.
DR InterPro; IPR008266; Tyr_kinase_AS.
DR Pfam; PF07714; Pkinase_Tyr; 1.
DR Pfam; PF07647; SAM_2; 1.
DR Pfam; PF00622; SPRY; 3.
DR SMART; SM00454; SAM; 1.
DR SMART; SM00449; SPRY; 3.
DR SUPFAM; SSF49899; ConA_like_lec_gl; 3.
DR SUPFAM; SSF56112; Kinase_like; 1.
DR SUPFAM; SSF47769; SAM_homology; 1.
DR PROSITE; PS50188; B302_SPRY; 3.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00109; PROTEIN_KINASE_TYR; 1.
DR PROSITE; PS50105; SAM_DOMAIN; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Complete proteome; Kinase; Nucleotide-binding;
KW Phosphoprotein; Reference proteome; Repeat; Transferase;
KW Tyrosine-protein kinase.
FT CHAIN 1 2410 Dual specificity protein kinase splA.
FT /FTId=PRO_0000088121.
FT DOMAIN 822 1004 B30.2/SPRY 1.
FT DOMAIN 1020 1209 B30.2/SPRY 2.
FT DOMAIN 1481 1703 B30.2/SPRY 3.
FT DOMAIN 1734 1798 SAM.
FT DOMAIN 2115 2387 Protein kinase.
FT NP_BIND 2121 2129 ATP (By similarity).
FT COMPBIAS 23 52 Poly-Asn.
FT COMPBIAS 162 320 Ser-rich.
FT COMPBIAS 213 219 Poly-Asn.
FT COMPBIAS 235 245 Poly-Asn.
FT COMPBIAS 321 352 Poly-Gln.
FT COMPBIAS 447 514 Poly-Gln.
FT COMPBIAS 515 648 Pro-rich.
FT COMPBIAS 601 610 Poly-Asn.
FT COMPBIAS 631 759 Ser-rich.
FT COMPBIAS 706 720 Poly-Gln.
FT COMPBIAS 777 813 Poly-Asn.
FT COMPBIAS 1229 1246 Poly-Asn.
FT COMPBIAS 1275 1306 Poly-Asn.
FT COMPBIAS 1309 1317 Poly-Asn.
FT COMPBIAS 1320 1358 Poly-Asn.
FT COMPBIAS 1422 1426 Poly-Asn.
FT COMPBIAS 1852 1855 Poly-Ser.
FT COMPBIAS 2021 2036 Poly-Asn.
FT COMPBIAS 2041 2046 Poly-Gln.
FT COMPBIAS 2050 2059 Poly-Gln.
FT COMPBIAS 2088 2103 Pro-rich.
FT ACT_SITE 2243 2243 Proton acceptor (By similarity).
FT BINDING 2142 2142 ATP (By similarity).
FT CONFLICT 2074 2074 D -> R (in Ref. 3; AAA33202).
FT CONFLICT 2261 2261 V -> L (in Ref. 3; AAA33202).
FT CONFLICT 2282 2282 M -> K (in Ref. 2; AAB41125 and 3;
FT AAA33202).
SQ SEQUENCE 2410 AA; 264919 MW; 768EC59E9E05C229 CRC64;
MNSKNDLFIG FFFFFYNYYY YYNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNIYIIVIIG
LDPQQNHPSL KIPPPPSPTS PYVRRHARQH SRSNSSNSPG ELTGGVEIIQ QQNSINTQTS
PPTSTSPNTV PPPPPTNTTT SSTTITRNSN NINNSNGGII NSSSGSNINN SSSSGVINTN
INNSGGNISP TSNSLPSSNN NILYTSSGSN SGNNNNNNNT INISSGSSGI NNSSNSNINN
NNNNNSSSSS GIHASGSLTA IPTNTNSNSS IFHSSNSVNR INKSNYSADS LVLPPNRISQ
VPQSQTQPQL QISPSTSFSS QQQQQQQLQQ QLQLQQQLQQ QIQQQQQIQQ QQNLHFTYSK
TFTSGQPNTL VNLSSPPPQS KHLHVSSDHS FFNEVLTPTP VIHNSTNQNN QLLFGDSDPL
SFLEYNNFIN RYQPALKNNQ PVSSYRQQQQ IQHQIQLQQI QQQQQQQQQI QQQQLQQQQQ
IQQQQIQQQQ QQIQQQQQQQ QQQQQQQQQL QQIQPPPTIQ PPPQQTTPTL RGNRSSGNLS
GLNSFSLKQS TDSLTPPPNS QQSTVSSNST PIAATPISPL TAPTSPPPPP PPPTNFNSKF
NNNNNNNINN SSNNNTTVPP SPPPIIVLPK SPSSRSPRPA SAPVPSAPFL VNNRVINTSS
SSNISTNNTD LNLSSSSSSS PPLNISTASP SKSEDSPPTV SPSYKQQQQQ QQQQQQQQQQ
LNNSSNSSYS PKPRSPSVSS PPPSSISPNS SPIGSPNISF HHHQQHQIPP PPPVLSNTNN
NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTNHTNKKEG DSSWFNMSFK FFKKKLVPSN
EYRWDLRKSN SLTLNIEDKS RCSYRLPTSG SKGIAKSTQP FSSSFTYFEL FITNGNGDKI
CFGLTTNDHP IEVYPGNYQG SYGYSGDGKC YFGTNEGRVY GPSFSSGDVV GCGYDSSSKT
LYFTKNGVYL GVAAQKVNLI GLYPTVGLQN PGESVVINFF GPFSYRGAPE KPSKQSTIKD
SGGSSIIPSE DLIPKEEFEV CRWSEKKNYH GKHVVVRNRT AFLPLDSPKD TIGGVRATQP
FGEGFCYFEV IIDQLDKGQL SIGLANLEYP TFYHVGWMPR SYGYHNDDGR KFRWREEPGV
NEGESYGSSY KKGDIIGCGL SFTSREIFFT KNGMYLGTAF SNVYGVFYPS VAFNEPGISI
TGVFGPPFKF SQVTLMLKNV NSTSILVPNG NNNNNSNNNN NNNNNNIIGN GKITTTTTTS
TSPSSINNNE DISSNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNSNSSNT NNNNINNTTN
NNNSNSNNNN NNNNSNSNSN SNNNNINNNN NNNNNNNNIY LTKKPSIGST DESSTGSLGG
NNSSGNNNSS SGSIGNNSSI IKQRSPPHSI NGPLMLPPSS TNNNNNIYSS YNSTTAGSST
TILPTLNHPI FGNTTSNNNS SSTLSVGGNN NLLGRHCQSL PITASTNHTL SSSLGVSFSS
PSSSPKTSPR KIVNSSEDLG FVQTFQDQDG QPPSAWRRCG KSIKTKDDIT LTIIKKKTSV
AMADRPFSSN SSSTICYFEV YLEGHDKKGS ITVGLSHSTY PFIKHIGREP KSYGFSSEGE
KYGGSEIGEP YGPFFFFDGD SIASSCVIGC GINTSTRDIF FTKNGHYLGV AFSRVTSDPL
YPSISFRGVV GGLCVATFPG GHFRFNIEDL PGISPSVWTE ALGPDRQGSG FKNWAPNDVA
IWLESFNYGQ YRKNFRDNNI SGRHLEGITH AMLKNDLGIE PYGHREDIIN RLNRMIQIWN
DKSPDSYPKI AIDSSDKIRW PASGGSSGGI NISGGVVIGS SSGSDDGITE ISSSSKNIRP
YKSYTQKEIE DRNRRSTISG GEKKNKYYID NQMDPHQIGS MDSDGLLPDF GQGPPDEKNS
SKTLSNEQIR YLQQRKDEPP IAISSTGNGG SVSSTGGSSG FLTFPSSNSL THPPQRDKPT
QEFTHLPPIT SNYKGITNTG QPHKSFDQPL ELFPRHSAFS NNGNNGNNNN NNNNNNIKAN
QQQQQQSSYQ QSQTQQQQQH ITSTSTSTTN KWIDPFGGWE TQSSLSHPPS RPPPPPPPPP
QLPVRSEYEI DFNELEFGQT IGKGFFGEVK RGYWRETDVA IKIIYRDQFK TKSSLVMFQN
EVGILSKLRH PNVVQFLGAC TAGGEDHHCI VTEWMGGGSL RQFLTDHFNL LEQNPHIRLK
LALDIAKGMN YLHGWTPPIL HRDLSSRNIL LDHNIDPKNP VVSSRQDIKC KISDFGLSRL
KMEQASQMTQ SVGCIPYMAP EVFKGDSNSE KSDVYSYGMV LFELLTSDEP QQDMKPMKMA
HLAAYESYRP PIPLTTSSKW KEILTQCWDS NPDSRPTFKQ IIVHLKEMED QGVSSFASVP
VQTIDTGVYA
//
