ID MBHL_RHILV Reviewed; 596 AA.
AC P18636;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 1.
DT 03-APR-2013, entry version 78.
DE RecName: Full=Uptake hydrogenase large subunit;
DE EC=1.12.99.6;
DE AltName: Full=Hydrogenlyase;
DE AltName: Full=Membrane-bound hydrogenase large subunit;
GN Name=hupB; Synonyms=hupL;
OS Rhizobium leguminosarum bv. viciae.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX NCBI_TaxID=387;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=B10;
RX PubMed=2402452; DOI=10.1093/nar/18.17.5285;
RA Schneider C.G., Schmitt H.J., Schild C., Tichy H.V., Lotz W.;
RT "DNA sequence encoding the two structural genes for the uptake
RT hydrogenase of Rhizobium leguminosarum bv. viciae B10.";
RL Nucleic Acids Res. 18:5285-5285(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=128c53;
RX PubMed=2103457; DOI=10.1007/BF00036924;
RA Hidalgo E., Leyva A., Ruiz-Argueso T.;
RT "Nucleotide sequence of the hydrogenase structural genes from
RT Rhizobium leguminosarum.";
RL Plant Mol. Biol. 15:367-370(1990).
CC -!- FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to
CC increase the production of ATP and to protect nitrogenase against
CC inhibition or damage by O(2) under carbon- or phosphate-limited
CC conditions.
CC -!- CATALYTIC ACTIVITY: H(2) + A = AH(2).
CC -!- COFACTOR: Binds 1 nickel ion per subunit (By similarity).
CC -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large
CC subunit family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; Z36981; CAA85431.1; -; Genomic_DNA.
DR EMBL; X52974; CAA37149.1; -; Genomic_DNA.
DR PIR; S11969; S11969.
DR ProteinModelPortal; P18636; -.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR GO; GO:0033748; F:hydrogenase (acceptor) activity; IEA:EC.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR InterPro; IPR001501; Ni-dep_hyd_lsu.
DR InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR Pfam; PF00374; NiFeSe_Hases; 1.
DR PROSITE; PS00507; NI_HGENASE_L_1; 1.
DR PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE 3: Inferred from homology;
KW Cell membrane; Membrane; Metal-binding; Nickel; Oxidoreductase.
FT CHAIN 1 596 Uptake hydrogenase large subunit.
FT /FTId=PRO_0000199715.
FT METAL 75 75 Nickel (Potential).
FT METAL 78 78 Nickel (Potential).
FT METAL 575 575 Nickel (Potential).
FT METAL 578 578 Nickel (Potential).
FT CONFLICT 510 510 R -> A (in Ref. 2; CAA37149).
SQ SEQUENCE 596 AA; 66193 MW; 474EA9B9FD2CBF68 CRC64;
MTIQTPNGFT LDNSGKRIVV DPVTRIEGHM RVEVNVDENN IIRNAVSTGT MWRGIEVILK
NRDPRDAWAF TERICGVCTG THALTSVRAV ENALGITIPD NANSIRNLMQ LALQVHDHVV
HFYHLHALDW VDVVSALSAD PKATSALAQS ISDWPLSSPG YFKDIQTRLK KFVESGQLGP
FKNGYWGNAS YKLPPEANLM AVAHYLEALD FQKEIVKIHT IFGGKNPHPN WLVGGVPCPI
NVDGTGAVGA INMERLNMVT SIIDQLIEFN DKVYVPDIMA IGSFYKDWLY GGGLSGKNVL
AYGDVPEHAN DYSEASLKLP RGAIINGNLA EVFPVDHADP EQIQEFVTHS WYKYPDESKG
LHPWDGITEP HYELGPNAKG TKTNIEQLDE GAKYSWIKAP RWRGNAMEVG PLARWVIGYA
QNKAEFKDPV DKVLKDLGLP VTALFSTLGR TAARALESQW AGYQMRYFQN KLIANIKAGD
SNTAFVDKWK PETWPKEVKG VGFTEAPRGR LAHWIRIKDG KIDNYQCVVP TTWNGSPRDP
TGNIGAFEAS LMDTPMSNPT QPLEILRTIH SFDPCLACST HVMSPDGQEM ARVQVR
//
