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Database: UniProt
Entry: P20261
LinkDB: P20261
Original site: P20261 
ID   LIP1_CANRU              Reviewed;         549 AA.
AC   P20261;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 3.
DT   26-NOV-2014, entry version 82.
DE   RecName: Full=Lipase 1;
DE            EC=3.1.1.3;
DE   Flags: Precursor;
GN   Name=LIP1;
OS   Candida rugosa (Yeast) (Candida cylindracea).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; mitosporic Saccharomycetales;
OC   Candida.
OX   NCBI_TaxID=5481;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 14830 / CBS 6330 / DSM 2031 / MS-5 / NRRL Y-17506;
RX   PubMed=1610906; DOI=10.1016/0167-4781(92)90085-E;
RA   Longhi S., Fusetti F., Grandori R., Lotti M., Vanoni M.,
RA   Alberghina L.;
RT   "Cloning and nucleotide sequences of two lipase genes from Candida
RT   cylindracea.";
RL   Biochim. Biophys. Acta 1131:227-232(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 12-549, AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=ATCC 14830 / CBS 6330 / DSM 2031 / MS-5 / NRRL Y-17506;
RX   PubMed=2506450; DOI=10.1038/341164a0;
RA   Kawaguchi Y., Honda H., Taniguchi-Morimura J., Iwasaki S.;
RT   "The codon CUG is read as serine in an asporogenic yeast Candida
RT   cylindracea.";
RL   Nature 341:164-166(1989).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.06 ANGSTROMS).
RX   PubMed=8509417;
RA   Grochulski P., Li Y., Schrag J.D., Bouthillier F., Smith P.,
RA   Harrison D., Rubin B., Cygler M.;
RT   "Insights into interfacial activation from an open structure of
RT   Candida rugosa lipase.";
RL   J. Biol. Chem. 268:12843-12847(1993).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS).
RX   PubMed=8142346; DOI=10.1021/bi00178a005;
RA   Grochulski P., Bouthillier F., Kazlauskas R.J., Serreqi A.N.,
RA   Schrag J.D., Ziomek E., Cygler M.;
RT   "Analogs of reaction intermediates identify a unique substrate binding
RT   site in Candida rugosa lipase.";
RL   Biochemistry 33:3494-3500(1994).
RN   [5]
RP   REVIEW.
RX   PubMed=9778794;
RX   DOI=10.1002/(SICI)1097-0061(19980915)14:12<1069::AID-YEA303>3.3.CO;2-B;
RA   Benjamin S., Pandey A.;
RT   "Candida rugosa lipases: molecular biology and versatility in
RT   biotechnology.";
RL   Yeast 14:1069-1087(1998).
CC   -!- CATALYTIC ACTIVITY: Triacylglycerol + H(2)O = diacylglycerol + a
CC       carboxylate.
CC   -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC       {ECO:0000305}.
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DR   EMBL; X64703; CAA45957.1; -; Genomic_DNA.
DR   EMBL; X16712; CAA34684.1; -; mRNA.
DR   PIR; S05684; S05684.
DR   PIR; S23448; S23448.
DR   PDB; 1CRL; X-ray; 2.06 A; A=16-549.
DR   PDB; 1LPM; X-ray; 2.18 A; A=1-549.
DR   PDB; 1LPN; X-ray; 2.18 A; A=1-549.
DR   PDB; 1LPO; X-ray; 2.18 A; A=1-549.
DR   PDB; 1LPP; X-ray; 2.18 A; A=1-549.
DR   PDB; 1LPS; X-ray; 2.18 A; A=1-549.
DR   PDB; 1TRH; X-ray; 2.10 A; A=16-549.
DR   PDB; 3RAR; X-ray; 2.19 A; A=16-549.
DR   PDBsum; 1CRL; -.
DR   PDBsum; 1LPM; -.
DR   PDBsum; 1LPN; -.
DR   PDBsum; 1LPO; -.
DR   PDBsum; 1LPP; -.
DR   PDBsum; 1LPS; -.
DR   PDBsum; 1TRH; -.
DR   PDBsum; 3RAR; -.
DR   ProteinModelPortal; P20261; -.
DR   SMR; P20261; 16-549.
DR   BRENDA; 3.1.1.3; 1139.
DR   EvolutionaryTrace; P20261; -.
DR   GO; GO:0004806; F:triglyceride lipase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002018; CarbesteraseB.
DR   InterPro; IPR019826; Carboxylesterase_B_AS.
DR   InterPro; IPR019819; Carboxylesterase_B_CS.
DR   Pfam; PF00135; COesterase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR   PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW   Hydrolase; Lipid degradation; Lipid metabolism; Signal.
FT   SIGNAL        1     15
FT   CHAIN        16    549       Lipase 1.
FT                                /FTId=PRO_0000008619.
FT   ACT_SITE    224    224       Acyl-ester intermediate.
FT   ACT_SITE    356    356       Charge relay system.
FT   ACT_SITE    464    464       Charge relay system.
FT   CARBOHYD    329    329       N-linked (GlcNAc...).
FT   CARBOHYD    366    366       N-linked (GlcNAc...).
FT   DISULFID     75    112
FT   DISULFID    283    292
FT   VARIANT     398    398       G -> Q.
FT   STRAND       18     20       {ECO:0000244|PDB:1CRL}.
FT   STRAND       26     28       {ECO:0000244|PDB:1CRL}.
FT   STRAND       33     42       {ECO:0000244|PDB:1CRL}.
FT   HELIX        49     51       {ECO:0000244|PDB:1CRL}.
FT   HELIX        84     86       {ECO:0000244|PDB:1TRH}.
FT   HELIX        88     97       {ECO:0000244|PDB:1CRL}.
FT   HELIX       100    105       {ECO:0000244|PDB:1CRL}.
FT   STRAND      106    108       {ECO:0000244|PDB:1TRH}.
FT   STRAND      114    119       {ECO:0000244|PDB:1CRL}.
FT   STRAND      129    135       {ECO:0000244|PDB:1CRL}.
FT   TURN        139    141       {ECO:0000244|PDB:1CRL}.
FT   HELIX       145    147       {ECO:0000244|PDB:1LPM}.
FT   HELIX       151    159       {ECO:0000244|PDB:1CRL}.
FT   STRAND      165    169       {ECO:0000244|PDB:1CRL}.
FT   HELIX       174    178       {ECO:0000244|PDB:1CRL}.
FT   HELIX       182    187       {ECO:0000244|PDB:1CRL}.
FT   HELIX       192    207       {ECO:0000244|PDB:1CRL}.
FT   HELIX       208    211       {ECO:0000244|PDB:1CRL}.
FT   STRAND      213    223       {ECO:0000244|PDB:1CRL}.
FT   HELIX       225    235       {ECO:0000244|PDB:1CRL}.
FT   HELIX       236    239       {ECO:0000244|PDB:1CRL}.
FT   STRAND      246    248       {ECO:0000244|PDB:1CRL}.
FT   STRAND      250    256       {ECO:0000244|PDB:1CRL}.
FT   HELIX       268    281       {ECO:0000244|PDB:1CRL}.
FT   HELIX       289    295       {ECO:0000244|PDB:1CRL}.
FT   HELIX       298    306       {ECO:0000244|PDB:1CRL}.
FT   TURN        314    317       {ECO:0000244|PDB:1CRL}.
FT   STRAND      327    330       {ECO:0000244|PDB:1CRL}.
FT   HELIX       334    339       {ECO:0000244|PDB:1CRL}.
FT   STRAND      348    353       {ECO:0000244|PDB:1CRL}.
FT   HELIX       358    361       {ECO:0000244|PDB:1CRL}.
FT   HELIX       362    364       {ECO:0000244|PDB:1CRL}.
FT   HELIX       370    380       {ECO:0000244|PDB:1CRL}.
FT   HELIX       386    395       {ECO:0000244|PDB:1CRL}.
FT   HELIX       400    402       {ECO:0000244|PDB:1CRL}.
FT   STRAND      403    405       {ECO:0000244|PDB:1CRL}.
FT   TURN        409    412       {ECO:0000244|PDB:1CRL}.
FT   STRAND      414    417       {ECO:0000244|PDB:1CRL}.
FT   HELIX       418    429       {ECO:0000244|PDB:1CRL}.
FT   HELIX       431    440       {ECO:0000244|PDB:1CRL}.
FT   STRAND      446    451       {ECO:0000244|PDB:1CRL}.
FT   TURN        453    456       {ECO:0000244|PDB:1CRL}.
FT   TURN        458    460       {ECO:0000244|PDB:1CRL}.
FT   STRAND      461    463       {ECO:0000244|PDB:1CRL}.
FT   HELIX       466    472       {ECO:0000244|PDB:1CRL}.
FT   HELIX       479    482       {ECO:0000244|PDB:1CRL}.
FT   HELIX       484    492       {ECO:0000244|PDB:1CRL}.
FT   HELIX       495    498       {ECO:0000244|PDB:1CRL}.
FT   STRAND      512    514       {ECO:0000244|PDB:1TRH}.
FT   STRAND      517    520       {ECO:0000244|PDB:1CRL}.
FT   STRAND      525    528       {ECO:0000244|PDB:1CRL}.
FT   HELIX       534    541       {ECO:0000244|PDB:1CRL}.
FT   HELIX       544    547       {ECO:0000244|PDB:1CRL}.
SQ   SEQUENCE   549 AA;  58550 MW;  27A40BD318757CE0 CRC64;
     MELALALSLI ASVAAAPTAT LANGDTITGL NAIINEAFLG IPFAEPPVGN LRFKDPVPYS
     GSLDGQKFTS YGPSCMQQNP EGTYEENLPK AALDLVMQSK VFEAVSPSSE DCLTINVVRP
     PGTKAGANLP VMLWIFGGGF EVGGTSTFPP AQMITKSIAM GKPIIHVSVN YRVSSWGFLA
     GDEIKAEGSA NAGLKDQRLG MQWVADNIAA FGGDPTKVTI FGESAGSMSV MCHILWNDGD
     NTYKGKPLFR AGIMQSGAMV PSDAVDGIYG NEIFDLLASN AGCGSASDKL ACLRGVSSDT
     LEDATNNTPG FLAYSSLRLS YLPRPDGVNI TDDMYALVRE GKYANIPVII GDQNDEGTFF
     GTSSLNVTTD AQAREYFKQS FVHASDAEID TLMTAYPGDI TQGSPFDTGI LNALTPQFKR
     ISAVLGDLGF TLARRYFLNH YTGGTKYSFL SKQLSGLPVL GTFHSNDIVF QDYLLGSGSL
     IYNNAFIAFA TDLDPNTAGL LVKWPEYTSS SQSGNNLMMI NALGLYTGKD NFRTAGYDAL
     FSNPPSFFV
//
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