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Database: UniProt
Entry: P21867
LinkDB: P21867
Original site: P21867 
ID   RAFR_ECOLX              Reviewed;         336 AA.
AC   P21867;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   01-OCT-2014, entry version 75.
DE   RecName: Full=HTH-type transcriptional regulator RafR;
DE   AltName: Full=Raffinose operon repressor;
GN   Name=rafR;
OS   Escherichia coli.
OG   Plasmid pRSD2.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-6.
RX   PubMed=2180920;
RA   Aslanidis C., Schmitt R.;
RT   "Regulatory elements of the raffinose operon: nucleotide sequences of
RT   operator and repressor genes.";
RL   J. Bacteriol. 172:2178-2180(1990).
CC   -!- FUNCTION: The raffinose (raf) operon is negatively controlled by
CC       the specific binding of raf repressor to raf operator (rafO) DNA.
CC       Binds raffinose as an inducer.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Contains 1 HTH lacI-type DNA-binding domain.
CC       {ECO:0000255|PROSITE-ProRule:PRU00111}.
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DR   EMBL; M29849; AAA24500.1; -; Genomic_DNA.
DR   PIR; A35160; A35160.
DR   ProteinModelPortal; P21867; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom.
DR   InterPro; IPR001761; Peripla_BP/Lac1_sug-bd_dom.
DR   InterPro; IPR028082; Peripla_BP_I.
DR   InterPro; IPR000843; Tscrpt_reg_HTH_LacI.
DR   Pfam; PF00356; LacI; 1.
DR   Pfam; PF00532; Peripla_BP_1; 1.
DR   SMART; SM00354; HTH_LACI; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   SUPFAM; SSF53822; SSF53822; 1.
DR   PROSITE; PS50932; HTH_LACI_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; DNA-binding; Plasmid; Repressor;
KW   Transcription; Transcription regulation.
FT   INIT_MET      1      1       Removed. {ECO:0000269|PubMed:2180920}.
FT   CHAIN         2    336       HTH-type transcriptional regulator RafR.
FT                                /FTId=PRO_0000107982.
FT   DOMAIN        2     55       HTH lacI-type. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00111}.
FT   DNA_BIND      3     22       H-T-H motif. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00111}.
FT   MUTAGEN      50     50       A->E: Loss of activity.
SQ   SEQUENCE   336 AA;  36774 MW;  60D313A0EDA71564 CRC64;
     MSLKAIATTL GISVTTVSRA LGGFSDVAAS TRERVEAEAR RRGYRPNTQA RRLKTGKTDA
     IGLVYPENDV PFNSGVFMDM VSCISRELAY HDIDLLLIAD DEHADCHSYM RLVESRRIDA
     LIIAHTLDDD PRITHLHKAG IPFLALGRVP QGLPCAWFDF DNHAGTWQAT QKLIALGHKS
     IALLSENTSH SYVIARRQGW LDALHEHGLK DPLLRLVSPT RRAGYLAVME LMSLPAPPTA
     IITDNDLSGD GAAMALQLRG RLSGKEAVSL VVYDGLPQDS IIELDVAAVI QSTRSLVGRQ
     ISDMVYQIIN GASPESLQIT WTPIFYPGST VHSPSF
//
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