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Database: UniProt
Entry: P23007
LinkDB: P23007
Original site: P23007 
ID   CISY_CHICK              Reviewed;         433 AA.
AC   P23007;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   01-OCT-2014, entry version 101.
DE   RecName: Full=Citrate synthase, mitochondrial;
DE            EC=2.3.3.1;
DE   AltName: Full=Citrate (Si)-synthase;
GN   Name=CS;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Testudines + Archosauria group; Archosauria; Dinosauria; Saurischia;
OC   Theropoda; Coelurosauria; Aves; Neognathae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), AND CATALYTIC ACTIVITY.
RC   TISSUE=Heart muscle;
RX   PubMed=2337600; DOI=10.1021/bi00461a002;
RA   Karpusas M., Branchaud B., Remington S.J.;
RT   "Proposed mechanism for the condensation reaction of citrate synthase:
RT   1.9-A structure of the ternary complex with oxaloacetate and
RT   carboxymethyl coenzyme A.";
RL   Biochemistry 29:2213-2219(1990).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF OPEN CONFORMATION.
RX   PubMed=2043641; DOI=10.1021/bi00238a029;
RA   Liao D.-I., Karpusas M., Remington S.J.;
RT   "Crystal structure of an open conformation of citrate synthase from
RT   chicken heart at 2.8-A resolution.";
RL   Biochemistry 30:6031-6036(1991).
CC   -!- CATALYTIC ACTIVITY: Acetyl-CoA + H(2)O + oxaloacetate = citrate +
CC       CoA. {ECO:0000255|PROSITE-ProRule:PRU10117,
CC       ECO:0000269|PubMed:2337600}.
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle;
CC       isocitrate from oxaloacetate: step 1/2.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells
CC       capable of oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
CC   -!- CAUTION: This is an X-ray determined sequence which was
CC       established using the sequence of pig citrate synthase and
CC       modifying it based on the observed electron density.
CC       {ECO:0000305}.
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DR   PDB; 1AL6; X-ray; 1.85 A; A=1-433.
DR   PDB; 1AMZ; X-ray; 1.80 A; A=3-433.
DR   PDB; 1CSC; X-ray; 1.70 A; A=1-433.
DR   PDB; 1CSH; X-ray; 1.65 A; A=3-433.
DR   PDB; 1CSI; X-ray; 1.70 A; A=3-433.
DR   PDB; 1CSR; X-ray; 1.70 A; A=3-433.
DR   PDB; 1CSS; X-ray; 1.70 A; A=3-433.
DR   PDB; 2CSC; X-ray; 1.70 A; A=1-433.
DR   PDB; 3CSC; X-ray; 1.90 A; A=1-433.
DR   PDB; 4CSC; X-ray; 1.90 A; A=1-433.
DR   PDB; 5CSC; X-ray; 2.80 A; A/B=1-433.
DR   PDB; 5CTS; X-ray; 1.90 A; A=1-433.
DR   PDB; 6CSC; X-ray; 2.25 A; A/B=1-433.
DR   PDB; 6CTS; X-ray; 2.50 A; A=1-433.
DR   PDBsum; 1AL6; -.
DR   PDBsum; 1AMZ; -.
DR   PDBsum; 1CSC; -.
DR   PDBsum; 1CSH; -.
DR   PDBsum; 1CSI; -.
DR   PDBsum; 1CSR; -.
DR   PDBsum; 1CSS; -.
DR   PDBsum; 2CSC; -.
DR   PDBsum; 3CSC; -.
DR   PDBsum; 4CSC; -.
DR   PDBsum; 5CSC; -.
DR   PDBsum; 5CTS; -.
DR   PDBsum; 6CSC; -.
DR   PDBsum; 6CTS; -.
DR   PRIDE; P23007; -.
DR   HOVERGEN; HBG005336; -.
DR   PhylomeDB; P23007; -.
DR   Reactome; REACT_115536; The tricarboxylic acid cycle.
DR   UniPathway; UPA00223; UER00717.
DR   EvolutionaryTrace; P23007; -.
DR   GO; GO:0005759; C:mitochondrial matrix; ISS:UniProtKB.
DR   GO; GO:0005739; C:mitochondrion; ISS:AgBase.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; ISS:AgBase.
DR   GO; GO:0005975; P:carbohydrate metabolic process; ISS:UniProtKB.
DR   GO; GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0044281; P:small molecule metabolic process; TAS:Reactome.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; TAS:Reactome.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR002020; Citrate_synthase-like.
DR   InterPro; IPR016141; Citrate_synthase-like_core.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR010109; Citrate_synthase_euk.
DR   PANTHER; PTHR11739; PTHR11739; 1.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   TIGRFAMs; TIGR01793; cit_synth_euk; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Mitochondrion; Reference proteome;
KW   Transferase; Tricarboxylic acid cycle.
FT   CHAIN         1    433       Citrate synthase, mitochondrial.
FT                                /FTId=PRO_0000169983.
FT   ACT_SITE    274    274
FT   ACT_SITE    320    320
FT   ACT_SITE    375    375
FT   HELIX         6     28
FT   STRAND       31     34
FT   HELIX        38     42
FT   TURN         43     47
FT   STRAND       49     52
FT   STRAND       55     59
FT   TURN         60     62
FT   STRAND       63     66
FT   HELIX        71     77
FT   STRAND       85     87
FT   HELIX        89     98
FT   HELIX       104    117
FT   HELIX       122    130
FT   HELIX       137    147
FT   HELIX       148    151
FT   HELIX       153    159
FT   HELIX       164    166
FT   HELIX       167    194
FT   TURN        195    197
FT   HELIX       209    217
FT   HELIX       222    234
FT   STRAND      240    242
FT   HELIX       243    252
FT   TURN        253    255
FT   HELIX       258    269
FT   TURN        272    276
FT   HELIX       277    291
FT   HELIX       298    310
FT   HELIX       328    340
FT   HELIX       345    364
FT   HELIX       375    384
FT   HELIX       390    392
FT   HELIX       393    414
FT   STRAND      423    425
FT   HELIX       427    433
SQ   SEQUENCE   433 AA;  47378 MW;  6942294BA95A9E06 CRC64;
     ASSTNLKDVL AALIPKEQAR IKTFRQQHGG TALGQITVDM SYGGMRGMKG LVYETSVLDP
     DEGIRFRGFS IPECQKLLPK GGXGGEPLPE GLFWLLVTGQ IPTGAQVSWL SKEWAKRAAL
     PSHVVTMLDN FPTNLHPMSQ LSAAITALNS ESNFARAYAE GILRTKYWEM VYESAMDLIA
     KLPCVAAKIY RNLYRAGSSI GAIDSKLDWS HNFTNMLGYT DAQFTELMRL YLTIHSDHEG
     GNVSAHTSHL VGSALSDPYL SFAAAMNGLA GPLHGLANQE VLGWLAQLQK AXXXAGADAS
     LRDYIWNTLN SGRVVPGYGH AVLRKTDPRY TCQREFALKH LPGDPMFKLV AQLYKIVPNV
     LLEQGAAANP WPNVDAHSGV LLQYYGMTEM NYYTVLFGVS RALGVLAQLI WSRALGFPLE
     RPKSMSTDGL IAL
//
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