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Database: UniProt
Entry: P23611
LinkDB: P23611
Original site: P23611 
ID   IRF8_MOUSE              Reviewed;         424 AA.
AC   P23611;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   03-SEP-2014, entry version 125.
DE   RecName: Full=Interferon regulatory factor 8;
DE            Short=IRF-8;
DE   AltName: Full=Interferon consensus sequence-binding protein;
DE            Short=ICSBP;
GN   Name=Irf8; Synonyms=Icsbp, Icsbp1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=2111015; DOI=10.1073/pnas.87.10.3743;
RA   Driggers P.H., Ennist D.L., Gleason S.L., Mak W.-H., Marks M.S.,
RA   Levi B.-Z., Flanagan J.R., Appella E., Ozato K.;
RT   "An interferon gamma-regulated protein that binds the interferon-
RT   inducible enhancer element of major histocompatibility complex class I
RT   genes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:3743-3747(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Colon;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M.,
RA   Davis M.J., Wilming L.G., Aidinis V., Allen J.E.,
RA   Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L.,
RA   Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M.,
RA   Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R.,
RA   Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G.,
RA   di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G.,
RA   Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M.,
RA   Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N.,
RA   Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T.,
RA   Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H.,
RA   Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K.,
RA   Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J.,
RA   Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L.,
RA   Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K.,
RA   Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P.,
RA   Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O.,
RA   Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G.,
RA   Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M.,
RA   Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B.,
RA   Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K.,
RA   Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A.,
RA   Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K.,
RA   Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C.,
RA   Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J.,
RA   Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y.,
RA   Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T.,
RA   Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N.,
RA   Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N.,
RA   Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S.,
RA   Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J.,
RA   Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Mammary gland;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   INTERACTION WITH TRIM21, UBIQUITINATION, INDUCTION, AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=17579016; DOI=10.4049/jimmunol.179.1.26;
RA   Kong H.J., Anderson D.E., Lee C.H., Jang M.K., Tamura T., Tailor P.,
RA   Cho H.K., Cheong J., Xiong H., Morse H.C. III, Ozato K.;
RT   "Autoantigen Ro52 is an interferon inducible E3 ligase that
RT   ubiquitinates IRF-8 and enhances cytokine expression in macrophages.";
RL   J. Immunol. 179:26-30(2007).
RN   [5]
RP   FUNCTION, AND INTERACTION WITH BATF.
RX   PubMed=22992524; DOI=10.1038/nature11531;
RA   Tussiwand R., Lee W.L., Murphy T.L., Mashayekhi M., Kc W.,
RA   Albring J.C., Satpathy A.T., Rotondo J.A., Edelson B.T., Kretzer N.M.,
RA   Wu X., Weiss L.A., Glasmacher E., Li P., Liao W., Behnke M., Lam S.S.,
RA   Aurthur C.T., Leonard W.J., Singh H., Stallings C.L., Sibley L.D.,
RA   Schreiber R.D., Murphy K.M.;
RT   "Compensatory dendritic cell development mediated by BATF-IRF
RT   interactions.";
RL   Nature 490:502-507(2012).
CC   -!- FUNCTION: Specifically binds to the upstream regulatory region of
CC       type I IFN and IFN-inducible MHC class I genes (the interferon
CC       consensus sequence (ICS)). Plays a regulatory role in cells of the
CC       immune system. Involved in CD8(+) dendritic cell differentiation
CC       by forming a complex with the BATF-JUNB heterodimer in immune
CC       cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-
CC       3'), an immune-specific regulatory element, followed by
CC       cooperative binding of BATF and IRF8 and activation of genes.
CC   -!- SUBUNIT: Interacts with COPS2 (By similarity). Interacts (via C-
CC       terminus) with TRIM21 (via C-terminus). Interacts with the BATF-
CC       JUNB heterodimer. Interacts with BATF (via bZIP domain); the
CC       interaction is direct.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: Predominantly in lymphoid tissues.
CC   -!- INDUCTION: By interferon gamma.
CC   -!- PTM: Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a
CC       process that is strongly increased upon interferon gamma
CC       stimulation, leds to the enhanced transcriptional activity of
CC       target cytokine genes.
CC   -!- SIMILARITY: Belongs to the IRF family.
CC   -!- SIMILARITY: Contains 1 IRF tryptophan pentad repeat DNA-binding
CC       domain.
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DR   EMBL; M32489; AAA37878.1; -; mRNA.
DR   EMBL; AK018533; BAB31258.1; -; mRNA.
DR   EMBL; BC005450; AAH05450.1; -; mRNA.
DR   CCDS; CCDS22721.1; -.
DR   PIR; A35861; A35861.
DR   RefSeq; NP_032346.1; NM_008320.3.
DR   RefSeq; XP_006530767.1; XM_006530704.1.
DR   UniGene; Mm.334861; -.
DR   ProteinModelPortal; P23611; -.
DR   SMR; P23611; 9-115, 198-377.
DR   BioGrid; 200504; 6.
DR   IntAct; P23611; 1.
DR   PhosphoSite; P23611; -.
DR   MaxQB; P23611; -.
DR   PaxDb; P23611; -.
DR   PRIDE; P23611; -.
DR   Ensembl; ENSMUST00000047737; ENSMUSP00000040245; ENSMUSG00000041515.
DR   Ensembl; ENSMUST00000162001; ENSMUSP00000125029; ENSMUSG00000041515.
DR   GeneID; 15900; -.
DR   KEGG; mmu:15900; -.
DR   UCSC; uc009nrk.1; mouse.
DR   CTD; 3394; -.
DR   MGI; MGI:96395; Irf8.
DR   eggNOG; NOG42878; -.
DR   HOGENOM; HOG000010107; -.
DR   HOVERGEN; HBG003072; -.
DR   InParanoid; P23611; -.
DR   KO; K10155; -.
DR   OMA; RELQQFY; -.
DR   OrthoDB; EOG7CCBR1; -.
DR   PhylomeDB; P23611; -.
DR   TreeFam; TF328512; -.
DR   Reactome; REACT_198660; Interferon gamma signaling.
DR   NextBio; 288616; -.
DR   PRO; PR:P23611; -.
DR   ArrayExpress; P23611; -.
DR   Bgee; P23611; -.
DR   CleanEx; MM_IRF8; -.
DR   Genevestigator; P23611; -.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0003677; F:DNA binding; IDA:MGI.
DR   GO; GO:0005515; F:protein binding; IPI:UniProtKB.
DR   GO; GO:0000975; F:regulatory region DNA binding; IEA:InterPro.
DR   GO; GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:MGI.
DR   GO; GO:0071222; P:cellular response to lipopolysaccharide; IMP:MGI.
DR   GO; GO:0042742; P:defense response to bacterium; IMP:MGI.
DR   GO; GO:0042832; P:defense response to protozoan; IMP:MGI.
DR   GO; GO:0006955; P:immune response; IMP:MGI.
DR   GO; GO:0030099; P:myeloid cell differentiation; IMP:MGI.
DR   GO; GO:0044130; P:negative regulation of growth of symbiont in host; IMP:MGI.
DR   GO; GO:0006909; P:phagocytosis; IMP:MGI.
DR   GO; GO:0032729; P:positive regulation of interferon-gamma production; IMP:MGI.
DR   GO; GO:0032735; P:positive regulation of interleukin-12 production; IMP:MGI.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IDA:MGI.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IPI:MGI.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:MGI.
DR   GO; GO:0009617; P:response to bacterium; IMP:MGI.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 2.60.200.10; -; 1.
DR   InterPro; IPR019817; Interferon_reg_fac_CS.
DR   InterPro; IPR001346; Interferon_reg_fact_DNA-bd_dom.
DR   InterPro; IPR019471; Interferon_reg_factor-3.
DR   InterPro; IPR017855; SMAD_dom-like.
DR   InterPro; IPR008984; SMAD_FHA_domain.
DR   InterPro; IPR011991; WHTH_DNA-bd_dom.
DR   Pfam; PF00605; IRF; 1.
DR   Pfam; PF10401; IRF-3; 1.
DR   PRINTS; PR00267; INTFRNREGFCT.
DR   SMART; SM00348; IRF; 1.
DR   SUPFAM; SSF49879; SSF49879; 1.
DR   PROSITE; PS00601; IRF_1; 1.
DR   PROSITE; PS51507; IRF_2; 1.
PE   1: Evidence at protein level;
KW   Activator; Complete proteome; DNA-binding; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation;
KW   Ubl conjugation.
FT   CHAIN         1    424       Interferon regulatory factor 8.
FT                                /FTId=PRO_0000154565.
FT   DNA_BIND      7    114       IRF tryptophan pentad repeat.
SQ   SEQUENCE   424 AA;  48237 MW;  FBE79A76846E8EB2 CRC64;
     MCDRNGGRRL RQWLIEQIDS SMYPGLIWEN DEKTMFRIPW KHAGKQDYNQ EVDASIFKAW
     AVFKGKFKEG DKAEPATWKT RLRCALNKSP DFEEVTDRSQ LDISEPYKVY RIVPEEEQKC
     KLGVAPAGCM SEVPEMECGR SEIEELIKEP SVDEYMGMTK RSPSPPEACR SQILPDWWVQ
     QPSAGLPLVT GYAAYDTHHS AFSQMVISFY YGGKLVGQAT TTCLEGCRLS LSQPGLPKLY
     GPDGLEPVCF PTADTIPSER QRQVTRKLFG HLERGVLLHS NRKGVFVKRL CQGRVFCSGN
     AVVCKGRPNK LERDEVVQVF DTNQFIRELQ QFYATQSRLP DSRVVLCFGE EFPDTVPLRS
     KLILVQVEQL YARQLVEEAG KSCGAGSLMP ALEEPQPDQA FRMFPDICTS HQRPFFRENQ
     QITV
//
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