ID MTS9_STAAU Reviewed; 430 AA.
AC P23737;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 01-MAY-2013, entry version 84.
DE RecName: Full=Modification methylase Sau96I;
DE Short=M.Sau96I;
DE EC=2.1.1.37;
DE AltName: Full=Cytosine-specific methyltransferase Sau96I;
GN Name=sau96IM;
OS Staphylococcus aureus.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcus.
OX NCBI_TaxID=1280;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PS96;
RX PubMed=2204026; DOI=10.1093/nar/18.16.4659;
RA Szilak L., Venetianer P., Kiss A.;
RT "Cloning and nucleotide sequence of the genes coding for the Sau96I
RT restriction and modification enzymes.";
RL Nucleic Acids Res. 18:4659-4664(1990).
CC -!- FUNCTION: This methylase recognizes the double-stranded sequence
CC GGNCC, causes specific methylation on C-4 on both strands, and
CC protects the DNA from cleavage by the Sau96I endonuclease.
CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + DNA = S-adenosyl-L-
CC homocysteine + DNA containing 5-methylcytosine.
CC -!- SIMILARITY: Belongs to the C5-methyltransferase family.
CC -!- SIMILARITY: Contains 1 HTH cro/C1-type DNA-binding domain.
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DR EMBL; X53096; CAA37260.1; -; Genomic_DNA.
DR PIR; S12705; S12705.
DR ProteinModelPortal; P23737; -.
DR REBASE; 3496; M.Sau96I.
DR GO; GO:0003886; F:DNA (cytosine-5-)-methyltransferase activity; IEA:EC.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0090116; P:C-5 methylation of cytosine; IEA:GOC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR018117; C5_DNA_meth_AS.
DR InterPro; IPR001525; C5_MeTfrase.
DR InterPro; IPR001387; HTH.
DR InterPro; IPR010982; Lambda_DNA-bd_dom.
DR PANTHER; PTHR10629; PTHR10629; 1.
DR Pfam; PF00145; DNA_methylase; 1.
DR Pfam; PF01381; HTH_3; 1.
DR PRINTS; PR00105; C5METTRFRASE.
DR SMART; SM00530; HTH_XRE; 1.
DR SUPFAM; SSF47413; Lambda_like_DNA; 1.
DR TIGRFAMs; TIGR00675; dcm; 1.
DR PROSITE; PS00094; C5_MTASE_1; 1.
DR PROSITE; PS00095; C5_MTASE_2; 1.
DR PROSITE; PS50943; HTH_CROC1; 1.
PE 3: Inferred from homology;
KW DNA-binding; Methyltransferase; Restriction system;
KW S-adenosyl-L-methionine; Transcription; Transcription regulation;
KW Transferase.
FT CHAIN 1 430 Modification methylase Sau96I.
FT /FTId=PRO_0000087908.
FT DOMAIN 9 63 HTH cro/C1-type.
FT ACT_SITE 174 174 By similarity.
SQ SEQUENCE 430 AA; 49284 MW; 884FBD96F4890651 CRC64;
MRLNKGSIIE KMKNQNIKTQ TELAEKIDIS KSQLSFMFSD EYEPLKKNVI KLADVLKVSP
NDIILDEEDQ MPINSDFNRY DYKLDEFIDV SNVRKNKDYN VFETFAGAGG LALGLESAGL
STYGAVEIDK NAAETLRINR PKWKVIENDI EFIADNLDEF IDEEIDILSG GYPCQTFSYA
GKRNGFADTR GTLFYPYSKI LSKLKPKAFI AENVRGLVNH DDGKTLEVML KVFIKEGYEV
YWNILNSWNY DVAQKRERIV IIGIREDLVK EQKYPFRFPL AQVYKPVLKD VLKDVPKSKV
TAYSDKKREV MKLVPPGGCW VDLPEQIAKD YMGKSWYSGG GKRGMARRIS WDEPCLTLTT
SPSQKQTERC HPDETRPFSI REYARIQSFP DEWEFSGGVG AQYRQIGNAV PVNLAKYIGK
SLVHYLNQFN
//
