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Database: UniProt
Entry: P24164
LinkDB: P24164
Original site: P24164 
ID   G3P_KLEPN               Reviewed;         303 AA.
AC   P24164;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 2.
DT   29-OCT-2014, entry version 89.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase;
DE            Short=GAPDH;
DE            EC=1.2.1.12;
DE   Flags: Fragment;
GN   Name=gap;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 13883 / DSM 30104 / JCM 1662 / NBRC 14940 / NCIMB 13281 /
RC   NCTC 9633;
RX   PubMed=1862091; DOI=10.1073/pnas.88.15.6667;
RA   Nelson K., Whittam T.S., Selander R.K.;
RT   "Nucleotide polymorphism and evolution in the glyceraldehyde-3-
RT   phosphate dehydrogenase gene (gapA) in natural populations of
RT   Salmonella and Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:6667-6671(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 10-303.
RC   STRAIN=LD119;
RX   PubMed=1955870;
RA   Lawrence J.G., Ochman H., Hartl D.L.;
RT   "Molecular and evolutionary relationships among enteric bacteria.";
RL   J. Gen. Microbiol. 137:1911-1921(1991).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC       {ECO:0000255|PROSITE-ProRule:PRU10009}.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 1/5.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family. {ECO:0000305}.
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DR   EMBL; M66869; AAA25069.1; ALT_TERM; Genomic_DNA.
DR   EMBL; M63371; AAA25068.1; -; Genomic_DNA.
DR   ProteinModelPortal; P24164; -.
DR   SMR; P24164; 1-303.
DR   UniPathway; UPA00109; UER00184.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IEA:UniProtKB-EC.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0006006; P:glucose metabolic process; IEA:InterPro.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.720; -; 1.
DR   InterPro; IPR020831; GlycerAld/Erythrose_P_DH.
DR   InterPro; IPR020830; GlycerAld_3-P_DH_AS.
DR   InterPro; IPR020829; GlycerAld_3-P_DH_cat.
DR   InterPro; IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DH_1.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   PANTHER; PTHR10836; PTHR10836; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   SMART; SM00846; Gp_dh_N; 1.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT   CHAIN        <1   >303       Glyceraldehyde-3-phosphate dehydrogenase.
FT                                /FTId=PRO_0000145664.
FT   NP_BIND       6      7       NAD. {ECO:0000250}.
FT   REGION      143    145       Glyceraldehyde 3-phosphate binding.
FT                                {ECO:0000250}.
FT   REGION      203    204       Glyceraldehyde 3-phosphate binding.
FT                                {ECO:0000250}.
FT   ACT_SITE    144    144       Nucleophile. {ECO:0000255|PROSITE-
FT                                ProRule:PRU10009}.
FT   BINDING      28     28       NAD. {ECO:0000250}.
FT   BINDING      72     72       NAD; via carbonyl oxygen. {ECO:0000250}.
FT   BINDING     174    174       Glyceraldehyde 3-phosphate.
FT                                {ECO:0000250}.
FT   BINDING     226    226       Glyceraldehyde 3-phosphate.
FT                                {ECO:0000250}.
FT   SITE        171    171       Activates thiol group during catalysis.
FT                                {ECO:0000250}.
FT   NON_TER       1      1
FT   NON_TER     303    303
SQ   SEQUENCE   303 AA;  32306 MW;  327B18AC56E39B2B CRC64;
     INGFGRIGRI VFRAAQKRSD IEIVAINDLL DAEYMAYMLK YDSTHGRFDG TVEVKDGHLV
     VNGKKIRVTA ERDPANLKWD EVGVDVVAEA TGIFLTDETA RKHITAGAKK VVLTGPSKDN
     TPMFVRGANF DAYAGQDIVS NASCTTNCLA PLAKVINDNF GIVEGLMTTV HATTATQKTV
     DGPSHKDWRG GRGAAQNIIP SSTGAAKAVG KVLPELNGKL TGMAFRVPTP NVSVVDLTVR
     LEKAASYEEI KKAIKAASEG AMKGVLGYTE DDVVSTDFNG EVCTSVFDAK AGIALNDNFV
     KLV
//
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