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Database: UniProt
Entry: P34185
LinkDB: P34185
Original site: P34185 
ID   TOP1_SYNE7              Reviewed;         883 AA.
AC   P34185; Q31NC3;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 2.
DT   29-OCT-2014, entry version 103.
DE   RecName: Full=DNA topoisomerase 1 {ECO:0000255|HAMAP-Rule:MF_00952};
DE            EC=5.99.1.2 {ECO:0000255|HAMAP-Rule:MF_00952};
DE   AltName: Full=DNA topoisomerase I {ECO:0000255|HAMAP-Rule:MF_00952};
DE   AltName: Full=Omega-protein;
DE   AltName: Full=Relaxing enzyme;
DE   AltName: Full=Swivelase;
DE   AltName: Full=Untwisting enzyme;
GN   Name=topA {ECO:0000255|HAMAP-Rule:MF_00952};
GN   OrderedLocusNames=Synpcc7942_1416;
OS   Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2).
OC   Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC   Synechococcus.
OX   NCBI_TaxID=1140;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7659748; DOI=10.1104/pp.108.4.1461;
RA   Ronen-Tarazi M., Lieman-Hurwitz J., Gabay C., Orus M.I., Kaplan A.;
RT   "The genomic region of rbcLS in Synechococcus sp. PCC 7942 contains
RT   genes involved in the ability to grow under low CO2 concentration and
RT   in chlorophyll biosynthesis.";
RL   Plant Physiol. 108:1461-1469(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7942;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Lykidis A., Richardson P.;
RT   "Complete sequence of chromosome 1 of Synechococcus elongatus PCC
RT   7942.";
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Releases the supercoiling and torsional tension of DNA,
CC       which is introduced during the DNA replication and transcription,
CC       by transiently cleaving and rejoining one strand of the DNA
CC       duplex. Introduces a single-strand break via transesterification
CC       at a target site in duplex DNA. The scissile phosphodiester is
CC       attacked by the catalytic tyrosine of the enzyme, resulting in the
CC       formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the
CC       expulsion of a 3'-OH DNA strand. The free DNA strand then
CC       undergoes passage around the unbroken strand, thus removing DNA
CC       supercoils. Finally, in the religation step, the DNA 3'-OH attacks
CC       the covalent intermediate to expel the active-site tyrosine and
CC       restore the DNA phosphodiester backbone. {ECO:0000255|HAMAP-
CC       Rule:MF_00952}.
CC   -!- CATALYTIC ACTIVITY: ATP-independent breakage of single-stranded
CC       DNA, followed by passage and rejoining. {ECO:0000255|HAMAP-
CC       Rule:MF_00952}.
CC   -!- COFACTOR: Magnesium. Binds two Mg(2+) per subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_00952}.
CC   -!- SUBUNIT: Monomer.
CC   -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00952}.
CC   -!- SIMILARITY: Contains 1 Toprim domain. {ECO:0000255|HAMAP-
CC       Rule:MF_00952}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA51086.1; Type=Frameshift; Positions=Several; Evidence={ECO:0000305};
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DR   EMBL; X72391; CAA51086.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; CP000100; ABB57446.1; -; Genomic_DNA.
DR   PIR; S32158; S32158.
DR   RefSeq; YP_400433.1; NC_007604.1.
DR   ProteinModelPortal; P34185; -.
DR   STRING; 1140.Synpcc7942_1416; -.
DR   EnsemblBacteria; ABB57446; ABB57446; Synpcc7942_1416.
DR   GeneID; 3774129; -.
DR   KEGG; syf:Synpcc7942_1416; -.
DR   PATRIC; 23788179; VBISynElo51371_1616.
DR   eggNOG; COG1754; -.
DR   HOGENOM; HOG000004020; -.
DR   KO; K03168; -.
DR   OrthoDB; EOG6S7XQ9; -.
DR   BioCyc; SYNEL:SYNPCC7942_1416-MONOMER; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003917; F:DNA topoisomerase type I activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0006265; P:DNA topological change; IEA:UniProtKB-HAMAP.
DR   Gene3D; 1.10.460.10; -; 2.
DR   Gene3D; 2.70.20.10; -; 2.
DR   Gene3D; 3.40.50.140; -; 1.
DR   HAMAP; MF_00952; Topoisom_1_prok; 1.
DR   InterPro; IPR000380; Topo_IA.
DR   InterPro; IPR003601; Topo_IA_2.
DR   InterPro; IPR023406; Topo_IA_AS.
DR   InterPro; IPR013497; Topo_IA_cen.
DR   InterPro; IPR013824; Topo_IA_cen_sub1.
DR   InterPro; IPR013825; Topo_IA_cen_sub2.
DR   InterPro; IPR023405; Topo_IA_core_domain.
DR   InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR   InterPro; IPR005733; TopoI_bac-type.
DR   InterPro; IPR028612; Topoisom_1_IA.
DR   InterPro; IPR025589; Toprim_C_rpt.
DR   InterPro; IPR006171; Toprim_domain.
DR   PANTHER; PTHR11390; PTHR11390; 1.
DR   Pfam; PF01131; Topoisom_bac; 1.
DR   Pfam; PF01751; Toprim; 1.
DR   Pfam; PF13368; Toprim_C_rpt; 3.
DR   PRINTS; PR00417; PRTPISMRASEI.
DR   SMART; SM00437; TOP1Ac; 1.
DR   SMART; SM00436; TOP1Bc; 1.
DR   SMART; SM00493; TOPRIM; 1.
DR   SUPFAM; SSF56712; SSF56712; 1.
DR   TIGRFAMs; TIGR01051; topA_bact; 1.
DR   PROSITE; PS00396; TOPOISOMERASE_I_PROK; 1.
DR   PROSITE; PS50880; TOPRIM; 1.
PE   3: Inferred from homology;
KW   Complete proteome; DNA-binding; Isomerase; Magnesium; Metal-binding;
KW   Reference proteome; Topoisomerase.
FT   CHAIN         1    883       DNA topoisomerase 1.
FT                                /FTId=PRO_0000145167.
FT   DOMAIN        2    126       Toprim. {ECO:0000255|HAMAP-
FT                                Rule:MF_00952}.
FT   REGION      175    180       Interaction with DNA. {ECO:0000255|HAMAP-
FT                                Rule:MF_00952}.
FT   ACT_SITE    320    320       O-(5'-phospho-DNA)-tyrosine intermediate.
FT                                {ECO:0000255|HAMAP-Rule:MF_00952}.
FT   METAL         8      8       Magnesium 1; catalytic.
FT                                {ECO:0000255|HAMAP-Rule:MF_00952}.
FT   METAL        95     95       Magnesium 1; catalytic.
FT                                {ECO:0000255|HAMAP-Rule:MF_00952}.
FT   METAL        95     95       Magnesium 2. {ECO:0000255|HAMAP-
FT                                Rule:MF_00952}.
FT   METAL        97     97       Magnesium 2. {ECO:0000255|HAMAP-
FT                                Rule:MF_00952}.
FT   SITE         32     32       Interaction with DNA. {ECO:0000255|HAMAP-
FT                                Rule:MF_00952}.
FT   SITE        151    151       Interaction with DNA. {ECO:0000255|HAMAP-
FT                                Rule:MF_00952}.
FT   SITE        152    152       Interaction with DNA. {ECO:0000255|HAMAP-
FT                                Rule:MF_00952}.
FT   SITE        155    155       Interaction with DNA. {ECO:0000255|HAMAP-
FT                                Rule:MF_00952}.
FT   SITE        160    160       Interaction with DNA. {ECO:0000255|HAMAP-
FT                                Rule:MF_00952}.
FT   SITE        167    167       Interaction with DNA. {ECO:0000255|HAMAP-
FT                                Rule:MF_00952}.
FT   SITE        322    322       Interaction with DNA. {ECO:0000255|HAMAP-
FT                                Rule:MF_00952}.
FT   SITE        515    515       Interaction with DNA. {ECO:0000255|HAMAP-
FT                                Rule:MF_00952}.
FT   CONFLICT    101    102       ES -> KV (in Ref. 1; CAA51086).
FT                                {ECO:0000305}.
FT   CONFLICT    392    392       L -> V (in Ref. 1; CAA51086).
FT                                {ECO:0000305}.
SQ   SEQUENCE   883 AA;  98296 MW;  C90DAC6EE1202B9A CRC64;
     MPKLVIVESP TKARTIRNYL PKDYRVEASM GHVRDLPQSA SDIPTELKGE KWSNLGVDVE
     NNFAPLYIVP KDKKKIVKTL KDALKDADEL ILATDEDREG ESISWHLLQL LQPRVPTKRM
     VFHEITQEAI QAALKNCRDV DQRLVHAQET RRILDRLVGY TLSPLLWKKI AWGLSAGRVQ
     SVAVRLLVQR ERARRAFRQG SYWDLKAQLT VEAGQFEAKL WTLAGQRLAT GNDFDESTGQ
     IIAGRQVCLL DQQEAEALRD RLQTQPWQVK SLEEKPTTRK PAPPFTTSTL QQESNRKLRL
     SARETMRVAQ SLYERGFITY MRTDSVHLSQ QAIEAARSCV EQMYGKNYLS PQPRQFTTKS
     KNAQEAHEAI RPAGNTFRLP QETGLSGAEF ALYDLIWKRT IASQMAEARQ TMLSVLLEVD
     NAEFRASGKR IDFPGFFRAY VEGSDDPDAA LEDREILLPA LKVGDRPTCQ ELAAIGHETQ
     PPARYTEASL VKMLENEGIG RPSTYASIIG TIVDRGYAQL VSNTLTPTFT AFAVTALLEQ
     HFPDLVDTSF SARMEQSLDD ISNGEVDWLP YLSQFYRGDR GLEEQVKLRE SEIDPAAART
     VALEGLPAKV RIGRFGAYLE AEADGEPIKA NLPKELTPAD LDVQRVETLL RQKTEGPDQL
     GTHPETDEPI YLLTGAYGPY VQLGAATEEK PKPKRASLPK GMSLETISLE QAVGLLSLPR
     TLGEHPETGR RIQAGLGRFG PYVVCDLGGG EKDYRSLKAD DDVLTIDLDR ALELLAQPKK
     SRGRGKEPIR EVGLHPDDQA PIQIFEGPYG LYLKHGKVNA SLPEDEKPET ISLETAVAAL
     AAKAGQAKAK GGRRSTGTPK SGETKARTTK TTKKTTTRRT TSR
//
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