ID RB11A_DICDI Reviewed; 214 AA.
AC P36412; Q55CC4;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 01-MAY-2013, entry version 93.
DE RecName: Full=Ras-related protein Rab-11A;
GN Name=rab11A; Synonyms=rab11; ORFNames=DDB_G0269238;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP MUTAGENESIS OF ASN-126.
RC STRAIN=AX3;
RX PubMed=11686306;
RA Harris E., Yoshida K., Cardelli J.A., Bush J.M. IV;
RT "Rab11-like GTPase associates with and regulates the structure and
RT function of the contractile vacuole system in dictyostelium.";
RL J. Cell Sci. 114:3035-3045(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=AX2;
RX PubMed=16926386; DOI=10.1074/mcp.M600113-MCP200;
RA Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA Soldati T.;
RT "Proteomics fingerprinting of phagosome maturation and evidence for
RT the role of a Galpha during uptake.";
RL Mol. Cell. Proteomics 5:2228-2243(2006).
CC -!- FUNCTION: Required for normal contractile vacuole structure and
CC function. Cells expressing a dominant negative rab11A exhibit a
CC more extensive contractile vacuole network and enlarged
CC contractile vacuole bladders. These cells exhibit a functional
CC defect in osmotic regulation where cells immersed in water become
CC rounded and detach from the surface, and contain swollen
CC contractile vacuoles.
CC -!- SUBCELLULAR LOCATION: Contractile vacuole membrane; Lipid-anchor;
CC Cytoplasmic side.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
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DR EMBL; U02925; AAA80149.1; -; mRNA.
DR EMBL; AAFI02000005; EAL71969.1; -; Genomic_DNA.
DR RefSeq; XP_646557.1; XM_641465.1.
DR ProteinModelPortal; P36412; -.
DR SMR; P36412; 10-180.
DR MINT; MINT-6947977; -.
DR STRING; 44689.DDB_0191190; -.
DR PRIDE; P36412; -.
DR EnsemblProtists; DDB0191190; DDB0191190; DDB_G0269238.
DR GeneID; 8617525; -.
DR KEGG; ddi:DDB_G0269238; -.
DR dictyBase; DDB_G0269238; rab11A.
DR eggNOG; COG1100; -.
DR KO; K07976; -.
DR OMA; AMGTRDD; -.
DR ProtClustDB; CLSZ2431439; -.
DR GO; GO:0031164; C:contractile vacuolar membrane; IDA:UniProtKB.
DR GO; GO:0045335; C:phagocytic vesicle; IDA:dictyBase.
DR GO; GO:0005525; F:GTP binding; IDA:UniProtKB.
DR GO; GO:0007231; P:osmosensory signaling pathway; IMP:UniProtKB.
DR GO; GO:0034394; P:protein localization to cell surface; IMP:dictyBase.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IDA:UniProtKB.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR003579; Small_GTPase_Rab_type.
DR Pfam; PF00071; Ras; 1.
DR PRINTS; PR00449; RASTRNSFRMNG.
DR SMART; SM00175; RAB; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 1: Evidence at protein level;
KW Complete proteome; GTP-binding; Lipoprotein; Membrane;
KW Nucleotide-binding; Prenylation; Protein transport;
KW Reference proteome; Transport; Vacuole.
FT CHAIN 1 214 Ras-related protein Rab-11A.
FT /FTId=PRO_0000121274.
FT NP_BIND 20 27 GTP (By similarity).
FT NP_BIND 68 72 GTP (By similarity).
FT NP_BIND 126 129 GTP (By similarity).
FT MOTIF 42 50 Effector region (By similarity).
FT LIPID 213 213 S-geranylgeranyl cysteine (By
FT similarity).
FT LIPID 214 214 S-geranylgeranyl cysteine (By
FT similarity).
FT MUTAGEN 126 126 N->I: Loss of GTP-binding activity.
SQ SEQUENCE 214 AA; 23986 MW; 6A82CA65688B5FE0 CRC64;
MTSKGSQEEY DYLYKIVLIG DSGVGKSNLL SRFTRNEFSL ETKSTIGVEF ATRTIQTEGK
TIKAQVWDTA GQERYRAITS AYYRGAVGAL LVYDIAKQAT YKSVERWILE LRENADRNIE
IMLVGNKSDL RHLREVSTDE AKEFSEKHKL TFIETSALDS SNVELAFQNI LTQIYHIMSR
PSHSTGPQTT IDSNTETIIL PTTSEPPAAK SGCC
//
