ID SER33_YEAST Reviewed; 469 AA.
AC P40510; D6VVL0;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 01-MAY-2013, entry version 124.
DE RecName: Full=D-3-phosphoglycerate dehydrogenase 2;
DE Short=3-PGDH 2;
DE EC=1.1.1.95;
GN Name=SER33; OrderedLocusNames=YIL074C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204511 / S288c / AB972;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N.,
RA Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G.,
RA Moule S., Odell C., Pearson D., Rajandream M.A., Rice P., Rowley N.,
RA Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RG Saccharomyces Genome Database;
RL Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA Dephoure N., O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22, AND MASS
RP SPECTROMETRY.
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22; THR-28; SER-29 AND
RP THR-31, AND MASS SPECTROMETRY.
RX PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth
RT phosphoproteome analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- CATALYTIC ACTIVITY: 3-phospho-D-glycerate + NAD(+) = 3-
CC phosphonooxypyruvate + NADH.
CC -!- CATALYTIC ACTIVITY: 2-hydroxyglutarate + NAD(+) = 2-oxoglutarate +
CC NADH.
CC -!- PATHWAY: Amino-acid biosynthesis; L-serine biosynthesis; L-serine
CC from 3-phospho-D-glycerate: step 1/3.
CC -!- INTERACTION:
CC Self; NbExp=3; IntAct=EBI-16821, EBI-16821;
CC P40054:SER3; NbExp=4; IntAct=EBI-16821, EBI-16961;
CC -!- MISCELLANEOUS: Present with 2010 molecules/cell in log phase SD
CC medium.
CC -!- SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid
CC dehydrogenase family.
CC -!- SIMILARITY: Contains 1 ACT domain.
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DR EMBL; Z37997; CAA86096.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08476.1; -; Genomic_DNA.
DR PIR; S48370; S48370.
DR RefSeq; NP_012191.1; NM_001179424.1.
DR ProteinModelPortal; P40510; -.
DR SMR; P40510; 59-469.
DR DIP; DIP-4483N; -.
DR IntAct; P40510; 5.
DR MINT; MINT-404061; -.
DR PaxDb; P40510; -.
DR PeptideAtlas; P40510; -.
DR PRIDE; P40510; -.
DR EnsemblFungi; YIL074C; YIL074C; YIL074C.
DR GeneID; 854736; -.
DR KEGG; sce:YIL074C; -.
DR CYGD; YIL074c; -.
DR SGD; S000001336; SER33.
DR eggNOG; COG0111; -.
DR GeneTree; ENSGT00530000063021; -.
DR HOGENOM; HOG000136696; -.
DR KO; K00058; -.
DR OMA; NLVVIGC; -.
DR OrthoDB; EOG4NGKWJ; -.
DR UniPathway; UPA00135; UER00196.
DR NextBio; 977440; -.
DR ArrayExpress; P40510; -.
DR Genevestigator; P40510; -.
DR GermOnline; YIL074C; Saccharomyces cerevisiae.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0004617; F:phosphoglycerate dehydrogenase activity; IMP:SGD.
DR GO; GO:0006564; P:L-serine biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009070; P:serine family amino acid biosynthetic process; IMP:SGD.
DR Gene3D; 3.40.50.720; -; 2.
DR InterPro; IPR006139; D-isomer_2_OHA_DH_cat_dom.
DR InterPro; IPR006140; D-isomer_2_OHA_DH_NAD-bd.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR Pfam; PF00389; 2-Hacid_dh; 1.
DR Pfam; PF02826; 2-Hacid_dh_C; 1.
DR PROSITE; PS00065; D_2_HYDROXYACID_DH_1; 1.
DR PROSITE; PS00670; D_2_HYDROXYACID_DH_2; 1.
DR PROSITE; PS00671; D_2_HYDROXYACID_DH_3; 1.
PE 1: Evidence at protein level;
KW Amino-acid biosynthesis; Complete proteome; NAD; Oxidoreductase;
KW Phosphoprotein; Reference proteome; Serine biosynthesis.
FT CHAIN 1 469 D-3-phosphoglycerate dehydrogenase 2.
FT /FTId=PRO_0000076019.
FT DOMAIN 398 468 ACT.
FT NP_BIND 208 209 NAD (By similarity).
FT NP_BIND 285 287 NAD (By similarity).
FT NP_BIND 347 350 NAD (By similarity).
FT ACT_SITE 287 287 By similarity.
FT ACT_SITE 316 316 By similarity.
FT ACT_SITE 347 347 Proton donor (By similarity).
FT BINDING 228 228 NAD (By similarity).
FT BINDING 311 311 NAD (By similarity).
FT MOD_RES 22 22 Phosphoserine.
FT MOD_RES 28 28 Phosphothreonine.
FT MOD_RES 29 29 Phosphoserine.
FT MOD_RES 31 31 Phosphothreonine.
SQ SEQUENCE 469 AA; 51188 MW; 23C59BAEA9FEDB36 CRC64;
MSYSAADNLQ DSFQRAMNFS GSPGAVSTSP TQSFMNTLPR RVSITKQPKA LKPFSTGDMN
ILLLENVNAT AIKIFKDQGY QVEFHKSSLP EDELIEKIKD VHAIGIRSKT RLTEKILQHA
RNLVCIGCFC IGTNQVDLKY AASKGIAVFN SPFSNSRSVA ELVIGEIISL ARQLGDRSIE
LHTGTWNKVA ARCWEVRGKT LGIIGYGHIG SQLSVLAEAM GLHVLYYDIV TIMALGTARQ
VSTLDELLNK SDFVTLHVPA TPETEKMLSA PQFAAMKDGA YVINASRGTV VDIPSLIQAV
KANKIAGAAL DVYPHEPAKN GEGSFNDELN SWTSELVSLP NIILTPHIGG STEEAQSSIG
IEVATALSKY INEGNSVGSV NFPEVSLKSL DYDQENTVRV LYIHRNVPGV LKTVNDILSD
HNIEKQFSDS HGEIAYLMAD ISSVNQSEIK DIYEKLNQTS AKVSIRLLY
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