ID TCYB_BACSU Reviewed; 234 AA.
AC P42200;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 01-MAY-2013, entry version 91.
DE RecName: Full=L-cystine transport system permease protein TcyB;
GN Name=tcyB; Synonyms=yckJ; OrderedLocusNames=BSU03600;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=7551042;
RA Rodriguez F., Grandi G.;
RT "An operon encoding a novel ABC-type transport system in Bacillus
RT subtilis.";
RL Microbiology 141:1781-1784(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA Yamane K., Kumano M., Kurita K.;
RT "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT determination of the sequence of a 146 kb segment and identification
RT of 113 genes.";
RL Microbiology 142:3047-3056(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP FUNCTION IN L-CYSTINE TRANSPORT, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC STRAIN=168;
RX PubMed=15262924; DOI=10.1128/JB.186.15.4875-4884.2004;
RA Burguiere P., Auger S., Hullo M.-F., Danchin A., Martin-Verstraete I.;
RT "Three different systems participate in L-cystine uptake in Bacillus
RT subtilis.";
RL J. Bacteriol. 186:4875-4884(2004).
CC -!- FUNCTION: Part of the ABC transporter complex TcyABC involved in
CC L-cystine import. Probably responsible for the translocation of
CC the substrate across the membrane (Probable).
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC Vmax=0.35 nmol/min/mg enzyme for L-cystine transport;
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins
CC (TcyC), two transmembrane proteins (TcyB) and a solute-binding
CC protein (TcyA) (Probable).
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein
CC (Potential).
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport
CC system permease family.
CC -!- SIMILARITY: Contains 1 ABC transmembrane type-1 domain.
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DR EMBL; X77636; CAA54725.1; -; Genomic_DNA.
DR EMBL; D50453; BAA08993.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12154.1; -; Genomic_DNA.
DR PIR; I40451; I40451.
DR RefSeq; NP_388242.1; NC_000964.3.
DR ProteinModelPortal; P42200; -.
DR STRING; 224308.BSU03600; -.
DR TCDB; 3.A.1.3.14; ATP-binding cassette (ABC) superfamily.
DR PaxDb; P42200; -.
DR EnsemblBacteria; CAB12154; CAB12154; BSU03600.
DR GeneID; 938301; -.
DR KEGG; bsu:BSU03600; -.
DR PATRIC; 18972287; VBIBacSub10457_0372.
DR GenoList; BSU03600; -.
DR eggNOG; COG0765; -.
DR HOGENOM; HOG000267552; -.
DR KO; K10009; -.
DR OMA; QHVIERR; -.
DR ProtClustDB; CLSK886684; -.
DR BioCyc; BSUB:BSU03600-MONOMER; -.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005215; F:transporter activity; IEA:InterPro.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR InterPro; IPR010065; AA_ABC_transptr_permease_3TM.
DR InterPro; IPR000515; BPD_transp.
DR Pfam; PF00528; BPD_transp_1; 1.
DR TIGRFAMs; TIGR01726; HEQRo_perm_3TM; 1.
DR PROSITE; PS50928; ABC_TM1; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell membrane; Complete proteome; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1 234 L-cystine transport system permease
FT protein TcyB.
FT /FTId=PRO_0000060267.
FT TRANSMEM 8 28 Helical; (Potential).
FT TRANSMEM 36 56 Helical; (Potential).
FT TRANSMEM 78 98 Helical; (Potential).
FT TRANSMEM 100 120 Helical; (Potential).
FT TRANSMEM 199 219 Helical; (Potential).
FT DOMAIN 32 221 ABC transmembrane type-1.
SQ SEQUENCE 234 AA; 26388 MW; 5790DCB40C4721A0 CRC64;
MFLNNLPALT LGTAIPWDLV QQSFWPILSG GIYYTIPLTI LSFIFGMILA LITALARMSK
VRPLRWVFSV YVSAIRGTPL LVQLFIIFYL FPAFNVTLDP FPSAVIAFSL NVGAYASEII
RASILSVPKG QWEAGYTIGM THQKTLFRVI LPQAFRVSIP PLSNTFISLI KDTSLASQIL
VAELFRKAQE IGARNLDQIL VIYIEAAFIY WIICFLLSLV QHVIERRLDR YVAK
//
