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Database: UniProt
Entry: P42200
LinkDB: P42200
Original site: P42200 
ID   TCYB_BACSU              Reviewed;         234 AA.
AC   P42200;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   09-JUL-2014, entry version 98.
DE   RecName: Full=L-cystine transport system permease protein TcyB;
GN   Name=tcyB; Synonyms=yckJ; OrderedLocusNames=BSU03600;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=7551042;
RA   Rodriguez F., Grandi G.;
RT   "An operon encoding a novel ABC-type transport system in Bacillus
RT   subtilis.";
RL   Microbiology 141:1781-1784(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA   Yamane K., Kumano M., Kurita K.;
RT   "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT   determination of the sequence of a 146 kb segment and identification
RT   of 113 genes.";
RL   Microbiology 142:3047-3056(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   FUNCTION IN L-CYSTINE TRANSPORT, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=168;
RX   PubMed=15262924; DOI=10.1128/JB.186.15.4875-4884.2004;
RA   Burguiere P., Auger S., Hullo M.-F., Danchin A., Martin-Verstraete I.;
RT   "Three different systems participate in L-cystine uptake in Bacillus
RT   subtilis.";
RL   J. Bacteriol. 186:4875-4884(2004).
CC   -!- FUNCTION: Part of the ABC transporter complex TcyABC involved in
CC       L-cystine import. Probably responsible for the translocation of
CC       the substrate across the membrane (Probable).
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         Vmax=0.35 nmol/min/mg enzyme for L-cystine transport;
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins
CC       (TcyC), two transmembrane proteins (TcyB) and a solute-binding
CC       protein (TcyA) (Probable).
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein
CC       (Potential).
CC   -!- SIMILARITY: Belongs to the binding-protein-dependent transport
CC       system permease family.
CC   -!- SIMILARITY: Contains 1 ABC transmembrane type-1 domain.
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DR   EMBL; X77636; CAA54725.1; -; Genomic_DNA.
DR   EMBL; D50453; BAA08993.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12154.1; -; Genomic_DNA.
DR   PIR; I40451; I40451.
DR   RefSeq; NP_388242.1; NC_000964.3.
DR   ProteinModelPortal; P42200; -.
DR   STRING; 224308.BSU03600; -.
DR   TCDB; 3.A.1.3.14; the atp-binding cassette (abc) superfamily.
DR   PaxDb; P42200; -.
DR   EnsemblBacteria; CAB12154; CAB12154; BSU03600.
DR   GeneID; 938301; -.
DR   KEGG; bsu:BSU03600; -.
DR   PATRIC; 18972287; VBIBacSub10457_0372.
DR   GenoList; BSU03600; -.
DR   eggNOG; COG0765; -.
DR   HOGENOM; HOG000267552; -.
DR   KO; K10009; -.
DR   OMA; STWMDIM; -.
DR   OrthoDB; EOG6MM1R5; -.
DR   PhylomeDB; P42200; -.
DR   BioCyc; BSUB:BSU03600-MONOMER; -.
DR   BioCyc; RETL1328306-WGS:GSTH-4097-MONOMER; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005215; F:transporter activity; IEA:InterPro.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.3720.10; -; 1.
DR   InterPro; IPR010065; AA_ABC_transptr_permease_3TM.
DR   InterPro; IPR000515; MetI-like.
DR   Pfam; PF00528; BPD_transp_1; 1.
DR   SUPFAM; SSF161098; SSF161098; 1.
DR   TIGRFAMs; TIGR01726; HEQRo_perm_3TM; 1.
DR   PROSITE; PS50928; ABC_TM1; 1.
PE   1: Evidence at protein level;
KW   Amino-acid transport; Cell membrane; Complete proteome; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN         1    234       L-cystine transport system permease
FT                                protein TcyB.
FT                                /FTId=PRO_0000060267.
FT   TRANSMEM      8     28       Helical; (Potential).
FT   TRANSMEM     36     56       Helical; (Potential).
FT   TRANSMEM     78     98       Helical; (Potential).
FT   TRANSMEM    100    120       Helical; (Potential).
FT   TRANSMEM    199    219       Helical; (Potential).
FT   DOMAIN       32    221       ABC transmembrane type-1.
SQ   SEQUENCE   234 AA;  26388 MW;  5790DCB40C4721A0 CRC64;
     MFLNNLPALT LGTAIPWDLV QQSFWPILSG GIYYTIPLTI LSFIFGMILA LITALARMSK
     VRPLRWVFSV YVSAIRGTPL LVQLFIIFYL FPAFNVTLDP FPSAVIAFSL NVGAYASEII
     RASILSVPKG QWEAGYTIGM THQKTLFRVI LPQAFRVSIP PLSNTFISLI KDTSLASQIL
     VAELFRKAQE IGARNLDQIL VIYIEAAFIY WIICFLLSLV QHVIERRLDR YVAK
//
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