ID G3P_HAEIN Reviewed; 339 AA.
AC P44304;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 01-MAY-2013, entry version 102.
DE RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase;
DE Short=GAPDH;
DE EC=1.2.1.12;
GN Name=gapA; Synonyms=gapDH; OrderedLocusNames=HI_0001;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M.,
RA Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D.,
RA Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C.,
RA Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M.,
RA Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O.,
RA Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
CC -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC glyceraldehyde 3-phosphate: step 1/5.
CC -!- SUBUNIT: Homotetramer (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC dehydrogenase family.
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DR EMBL; L42023; AAC21680.1; -; Genomic_DNA.
DR PIR; G64041; G64041.
DR RefSeq; NP_438174.1; NC_000907.1.
DR ProteinModelPortal; P44304; -.
DR SMR; P44304; 3-334.
DR STRING; 71421.HI0001; -.
DR PRIDE; P44304; -.
DR EnsemblBacteria; AAC21680; AAC21680; HI_0001.
DR GeneID; 950899; -.
DR KEGG; hin:HI0001; -.
DR PATRIC; 20188453; VBIHaeInf48452_0001.
DR eggNOG; COG0057; -.
DR KO; K00134; -.
DR OMA; QDFIGEV; -.
DR ProtClustDB; CLSK870299; -.
DR UniPathway; UPA00109; UER00184.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IEA:EC.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.720; -; 1.
DR InterPro; IPR020831; GlycerAld/Erythrose_P_DH.
DR InterPro; IPR020830; GlycerAld_3-P_DH_AS.
DR InterPro; IPR020829; GlycerAld_3-P_DH_cat.
DR InterPro; IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
DR InterPro; IPR006424; Glyceraldehyde-3-P_DH_1.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR PANTHER; PTHR10836; PTHR10836; 1.
DR Pfam; PF02800; Gp_dh_C; 1.
DR Pfam; PF00044; Gp_dh_N; 1.
DR PIRSF; PIRSF000149; GAP_DH; 1.
DR PRINTS; PR00078; G3PDHDRGNASE.
DR SMART; SM00846; Gp_dh_N; 1.
DR TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR PROSITE; PS00071; GAPDH; 1.
PE 3: Inferred from homology;
KW Complete proteome; Cytoplasm; Glycolysis; NAD; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1 339 Glyceraldehyde-3-phosphate dehydrogenase.
FT /FTId=PRO_0000145661.
FT NP_BIND 12 13 NAD (By similarity).
FT REGION 149 151 Glyceraldehyde 3-phosphate binding (By
FT similarity).
FT REGION 209 210 Glyceraldehyde 3-phosphate binding (By
FT similarity).
FT ACT_SITE 150 150 Nucleophile (By similarity).
FT BINDING 34 34 NAD (By similarity).
FT BINDING 78 78 NAD; via carbonyl oxygen (By similarity).
FT BINDING 180 180 Glyceraldehyde 3-phosphate (By
FT similarity).
FT BINDING 232 232 Glyceraldehyde 3-phosphate (By
FT similarity).
FT BINDING 319 319 NAD (By similarity).
FT SITE 177 177 Activates thiol group during catalysis
FT (By similarity).
SQ SEQUENCE 339 AA; 36052 MW; 87119EDEB114EDF0 CRC64;
MAIKIGINGF GRIGRIVFRA AQHRDDIEVV GINDLIDVEY MAYMLKYDST HGRFDGTVEV
KDGNLVVNGK TIRVTAERDP ANLNWGAIGV DIAVEATGLF LTDETARKHI TAGAKKVVLT
GPSKDATPMF VRGVNFNAYA GQDIVSNASC TTNCLAPLAR VVHETFGIKD GLMTTVHATT
ATQKTVDGPS AKDWRGGRGA SQNIIPSSTG AAKAVGKVLP ALNGKLTGMA FRVPTPNVSV
VDLTVNLEKP ASYDAIKQAI KDAAEGKTFN GELKGVLGYT EDAVVSTDFN GCALTSVFDA
DAGIALTDSF VKLVSWYDNE TGYSNKVLDL VAHIYNYKG
//
