GenomeNet

Database: UniProt
Entry: P44304
LinkDB: P44304
Original site: P44304 
ID   G3P_HAEIN               Reviewed;         339 AA.
AC   P44304;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   01-OCT-2014, entry version 108.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase;
DE            Short=GAPDH;
DE            EC=1.2.1.12;
GN   Name=gapA; Synonyms=gapDH; OrderedLocusNames=HI_0001;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M.,
RA   Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D.,
RA   Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C.,
RA   Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M.,
RA   Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O.,
RA   Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC       {ECO:0000255|PROSITE-ProRule:PRU10009}.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 1/5.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family. {ECO:0000305}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; L42023; AAC21680.1; -; Genomic_DNA.
DR   PIR; G64041; G64041.
DR   RefSeq; NP_438174.1; NC_000907.1.
DR   ProteinModelPortal; P44304; -.
DR   SMR; P44304; 3-334.
DR   STRING; 71421.HI0001; -.
DR   PRIDE; P44304; -.
DR   EnsemblBacteria; AAC21680; AAC21680; HI_0001.
DR   GeneID; 950899; -.
DR   KEGG; hin:HI0001; -.
DR   PATRIC; 20188453; VBIHaeInf48452_0001.
DR   eggNOG; COG0057; -.
DR   KO; K00134; -.
DR   OMA; NDKWGIE; -.
DR   OrthoDB; EOG66TG3S; -.
DR   PhylomeDB; P44304; -.
DR   UniPathway; UPA00109; UER00184.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IEA:UniProtKB-EC.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0006006; P:glucose metabolic process; IEA:InterPro.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.720; -; 1.
DR   InterPro; IPR020831; GlycerAld/Erythrose_P_DH.
DR   InterPro; IPR020830; GlycerAld_3-P_DH_AS.
DR   InterPro; IPR020829; GlycerAld_3-P_DH_cat.
DR   InterPro; IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DH_1.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   PANTHER; PTHR10836; PTHR10836; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   SMART; SM00846; Gp_dh_N; 1.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Glycolysis; NAD; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN         1    339       Glyceraldehyde-3-phosphate dehydrogenase.
FT                                /FTId=PRO_0000145661.
FT   NP_BIND      12     13       NAD. {ECO:0000250}.
FT   REGION      149    151       Glyceraldehyde 3-phosphate binding.
FT                                {ECO:0000250}.
FT   REGION      209    210       Glyceraldehyde 3-phosphate binding.
FT                                {ECO:0000250}.
FT   ACT_SITE    150    150       Nucleophile. {ECO:0000255|PROSITE-
FT                                ProRule:PRU10009}.
FT   BINDING      34     34       NAD. {ECO:0000250}.
FT   BINDING      78     78       NAD; via carbonyl oxygen. {ECO:0000250}.
FT   BINDING     180    180       Glyceraldehyde 3-phosphate.
FT                                {ECO:0000250}.
FT   BINDING     232    232       Glyceraldehyde 3-phosphate.
FT                                {ECO:0000250}.
FT   BINDING     319    319       NAD. {ECO:0000250}.
FT   SITE        177    177       Activates thiol group during catalysis.
FT                                {ECO:0000250}.
SQ   SEQUENCE   339 AA;  36052 MW;  87119EDEB114EDF0 CRC64;
     MAIKIGINGF GRIGRIVFRA AQHRDDIEVV GINDLIDVEY MAYMLKYDST HGRFDGTVEV
     KDGNLVVNGK TIRVTAERDP ANLNWGAIGV DIAVEATGLF LTDETARKHI TAGAKKVVLT
     GPSKDATPMF VRGVNFNAYA GQDIVSNASC TTNCLAPLAR VVHETFGIKD GLMTTVHATT
     ATQKTVDGPS AKDWRGGRGA SQNIIPSSTG AAKAVGKVLP ALNGKLTGMA FRVPTPNVSV
     VDLTVNLEKP ASYDAIKQAI KDAAEGKTFN GELKGVLGYT EDAVVSTDFN GCALTSVFDA
     DAGIALTDSF VKLVSWYDNE TGYSNKVLDL VAHIYNYKG
//
DBGET integrated database retrieval system