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Database: UniProt
Entry: P48418
LinkDB: P48418
Original site: P48418 
ID   C75A1_PETHY             Reviewed;         506 AA.
AC   P48418;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   01-OCT-2014, entry version 84.
DE   RecName: Full=Flavonoid 3',5'-hydroxylase 1;
DE            Short=F3'5'H;
DE            EC=1.14.13.88;
DE   AltName: Full=CYPLXXVA1;
DE   AltName: Full=Cytochrome P450 75A1;
GN   Name=CYP75A1; Synonyms=HF1;
OS   Petunia hybrida (Petunia).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Petunioideae;
OC   Petunia.
OX   NCBI_TaxID=4102;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Old Glory Blue; TISSUE=Petal;
RX   PubMed=8232589; DOI=10.1038/366276a0;
RA   Holton T.A., Brugliera F., Lester D.R., Tanaka Y., Hyland C.D.,
RA   Menting J.G.T., Lu C.-Y., Farcy E., Stevenson T.W., Cornish E.C.;
RT   "Cloning and expression of cytochrome P450 genes controlling flower
RT   colour.";
RL   Nature 366:276-279(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. Falcon Blue; TISSUE=Petal;
RA   Ohbayashi M., Shimada Y., Nakano R., Kiyokawa S., Kikuchi Y.;
RT   "Molecular cloning of cDNA encoding flavonoid-3',5'-hydroxylase from
RT   Petunia hybrida.";
RL   Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE.
RA   Hwang T.S., Chen Y.C., Wu R.Y.;
RT   "Cloning and sequencing of the genomic flavonoid 3',5'-hydroxylase
RT   (F3',5'Hase) gene from Petunia hybrida.";
RL   Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. Carnival violet; TISSUE=Petal;
RA   Baek S.H.;
RT   "Isolation and characterization of cytochrome P450 genes.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE OF 1-425.
RC   STRAIN=cv. Blue-star; TISSUE=Flower;
RA   Toguri T., Azuma M., Ohtani T.;
RT   "The cloning and characterization of a cDNA encoding a cytochrome P450
RT   from the flowers of Petunia hybrida.";
RL   Plant Sci. 94:119-126(1993).
CC   -!- FUNCTION: Catalyzes the 3'5'-hydroxylation of naringenin and
CC       eriodictyol to form 5,7,3,'4',5'-pentahydroxyflavanone and 3',5'-
CC       hydroxylation of dihydrokaempferol and dihydroquercetin to form
CC       dihydromyricetin.
CC   -!- CATALYTIC ACTIVITY: A flavanone + 2 NADPH + 2 O(2) = a 3',5'-
CC       dihydroxyflavanone + 2 NADP(+) + 2 H(2)O.
CC   -!- COFACTOR: Heme group. {ECO:0000250}.
CC   -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Microsome {ECO:0000305}. Endoplasmic
CC       reticulum {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Flowers.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; Z22545; CAA80266.1; -; mRNA.
DR   EMBL; D14588; BAA03438.1; -; mRNA.
DR   EMBL; AF081575; AAC32274.1; -; Genomic_DNA.
DR   EMBL; AY245545; AAO91941.1; -; mRNA.
DR   EMBL; X71130; CAA50442.1; -; mRNA.
DR   PIR; S32110; S32110.
DR   PIR; S38985; S38985.
DR   ProteinModelPortal; P48418; -.
DR   BioCyc; MetaCyc:MONOMER-12020; -.
DR   BRENDA; 1.14.13.88; 4700.
DR   UniPathway; UPA00009; -.
DR   GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR   GO; GO:0033772; F:flavonoid 3',5'-hydroxylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0009718; P:anthocyanin-containing compound biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Heme; Iron; Metal-binding; Microsome;
KW   Monooxygenase; NADP; Oxidoreductase.
FT   CHAIN         1    506       Flavonoid 3',5'-hydroxylase 1.
FT                                /FTId=PRO_0000052129.
FT   METAL       443    443       Iron (heme axial ligand). {ECO:0000250}.
SQ   SEQUENCE   506 AA;  56548 MW;  FCF3F9623BD82B3E CRC64;
     MMLLTELGAA TSIFLIAHII ISTLISKTTG RHLPPGPRGW PVIGALPLLG AMPHVSLAKM
     AKKYGAIMYL KVGTCGMAVA STPDAAKAFL KTLDINFSNR PPNAGATHLA YNAQDMVFAH
     YGPRWKLLRK LSNLHMLGGK ALENWANVRA NELGHMLKSM SDMSREGQRV VVAEMLTFAM
     ANMIGQVMLS KRVFVDKGVE VNEFKDMVVE LMTIAGYFNI GDFIPCLAWM DLQGIEKRMK
     RLHKKFDALL TKMFDEHKAT TYERKGKPDF LDVVMENGDN SEGERLSTTN IKALLLNLFT
     AGTDTSSSAI EWALAEMMKN PAILKKAQAE MDQVIGRNRR LLESDIPNLP YLRAICKETF
     RKHPSTPLNL PRISNEPCIV DGYYIPKNTR LSVNIWAIGR DPQVWENPLE FNPERFLSGR
     NSKIDPRGND FELIPFGAGR RICAGTRMGI VMVEYILGTL VHSFDWKLPS EVIELNMEEA
     FGLALQKAVP LEAMVTPRLQ LDVYVP
//
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