ID PCKA1_UROPA Reviewed; 624 AA.
AC P49292;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 27-MAR-2024, entry version 100.
DE RecName: Full=Phosphoenolpyruvate carboxykinase (ATP) 1;
DE EC=4.1.1.49;
GN Name=PCK1;
OS Urochloa panicoides (Panic liverseed grass).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Panicodae; Paniceae; Melinidinae; Urochloa.
OX NCBI_TaxID=37563;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 57-82.
RC STRAIN=CQ2798; TISSUE=Leaf;
RX PubMed=7888625; DOI=10.1007/bf00020190;
RA Finnegan P.M., Burnell J.N.;
RT "Isolation and sequence analysis of cDNAs encoding phosphoenolpyruvate
RT carboxykinase from the PCK-type C4 grass Urochloa panicoides.";
RL Plant Mol. Biol. 27:365-376(1995).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + oxaloacetate = ADP + CO2 + phosphoenolpyruvate;
CC Xref=Rhea:RHEA:18617, ChEBI:CHEBI:16452, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58702, ChEBI:CHEBI:456216;
CC EC=4.1.1.49;
CC -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC -!- SUBUNIT: Homohexamer.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- TISSUE SPECIFICITY: Green leaves but not in roots or etiolated shoots.
CC -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase (ATP)
CC family. {ECO:0000305}.
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DR EMBL; U09241; AAA79122.1; -; mRNA.
DR PIR; S52988; S52988.
DR AlphaFoldDB; P49292; -.
DR SMR; P49292; -.
DR UniPathway; UPA00138; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004612; F:phosphoenolpyruvate carboxykinase (ATP) activity; IEA:UniProtKB-EC.
DR GO; GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway.
DR CDD; cd00484; PEPCK_ATP; 1.
DR Gene3D; 3.90.228.20; -; 1.
DR Gene3D; 3.40.449.10; Phosphoenolpyruvate Carboxykinase, domain 1; 1.
DR Gene3D; 2.170.8.10; Phosphoenolpyruvate Carboxykinase, domain 2; 1.
DR HAMAP; MF_00453; PEPCK_ATP; 1.
DR InterPro; IPR001272; PEP_carboxykinase_ATP.
DR InterPro; IPR013035; PEP_carboxykinase_C.
DR InterPro; IPR008210; PEP_carboxykinase_N.
DR InterPro; IPR015994; PEPCK_ATP_CS.
DR NCBIfam; TIGR00224; pckA; 1.
DR PANTHER; PTHR30031:SF0; PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP); 1.
DR PANTHER; PTHR30031; PHOSPHOENOLPYRUVATE CARBOXYKINASE ATP; 1.
DR Pfam; PF01293; PEPCK_ATP; 1.
DR PIRSF; PIRSF006294; PEP_crbxkin; 1.
DR SUPFAM; SSF68923; PEP carboxykinase N-terminal domain; 1.
DR SUPFAM; SSF53795; PEP carboxykinase-like; 1.
DR PROSITE; PS00532; PEPCK_ATP; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cytoplasm; Decarboxylase; Direct protein sequencing;
KW Gluconeogenesis; Lyase; Nucleotide-binding.
FT CHAIN 1..624
FT /note="Phosphoenolpyruvate carboxykinase (ATP) 1"
FT /id="PRO_0000203865"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 322..329
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 624 AA; 68480 MW; 8B4831893C22ECB5 CRC64;
MASPNGGVTT YDYDDSDSAA PVRAQTIEEL HSLQRKAAAT AKDSASPLQS ISASLASTAR
EYGPNLVKGD PEAKGAPPAP VKHQQAAAAA AIAASDSSLK FTHVLYNLSP AELYEQAFGQ
KKSSFITSTG ALATLSGAKT GRSPRDKRVV KDDTTAQELW WGKGSPNIEM DERQFVINRE
RALDFLNSLD KVYVNDQFLN WDPENRIKVR IITSRAYHAL FMHNMCIRPT EEELETFGTP
DFTIYNAGEF PANRYANYMT SSTSINISLA RREMVILGTQ YAGEMKKGLF GVMHYLMPKR
GILSLHSGCN MGKEGDVALF FGLSGTGKTT LSTDHNRLLI GDDEHCWSDN GVSNIEGGCY
AKCIDLSKEK EPDIWNAITF GTVLENVVFN ERTREVDYAD KSITENTRAA YPIEFIPNAK
IPCVGPHPKN VILLACDAYG VLPPVSKLNL AQTMYHFISG YTAIVAGTED GVKEPTATFS
ACFGAAFIMY HPTKYAAMLA EKMQKYGRTG WLVNTGWSGG RYGVGNRIKL PYTRKIIDAI
HSGELLTANY KKTEVFGLEI PTEINGVPSE ILDPVNTWTD KAAYKETLLK LAGLFKNNFE
VFASYKIGDD NSLTEQILAA GPNF
//
