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Database: UniProt
Entry: P50178
LinkDB: P50178
Original site: P50178 
ID   MTL22_LACLC             Reviewed;         269 AA.
AC   P50178;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   11-JUN-2014, entry version 63.
DE   RecName: Full=Modification methylase LlaDCHIB;
DE            Short=M.LlaDCHI B;
DE            Short=M.LlaDCHIB;
DE            EC=2.1.1.72;
DE   AltName: Full=Adenine-specific methyltransferase LlaDCHIB;
DE   AltName: Full=M.LlaII B;
GN   Name=llaDCHIBM; Synonyms=llaIIB;
OS   Lactococcus lactis subsp. cremoris (Streptococcus cremoris).
OG   Plasmid pSRQ700.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=1359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DCH-4;
RX   PubMed=7793939;
RA   Moineau S., Walker S.A., Vedamuthu E.R., Vandenbergh P.A.;
RT   "Cloning and sequencing of LlaDCHI restriction/modification genes from
RT   Lactococcus lactis and relatedness of this system to the Streptococcus
RT   pneumoniae DpnII system.";
RL   Appl. Environ. Microbiol. 61:2193-2202(1995).
CC   -!- FUNCTION: This methylase recognizes the double-stranded sequence
CC       GATC, causes specific methylation on A-2 on both strands, and
CC       protects the DNA from cleavage by the LlaDCHI endonuclease.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + DNA adenine = S-
CC       adenosyl-L-homocysteine + DNA 6-methylaminopurine.
CC   -!- MISCELLANEOUS: The LlaII restriction system has two different
CC       methylases.
CC   -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
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DR   EMBL; U16027; AAB06312.1; -; Genomic_DNA.
DR   RefSeq; NP_116732.1; NC_002798.1.
DR   ProteinModelPortal; P50178; -.
DR   REBASE; 3663; M2.LlaDCHI.
DR   GeneID; 1113499; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR002941; DNA_methylase_N4/N6.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR001091; RM_Methylase.
DR   InterPro; IPR029063; SAM-dependent_MTases-like.
DR   Pfam; PF01555; N6_N4_Mtase; 1.
DR   PRINTS; PR00508; S21N4MTFRASE.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Plasmid; Restriction system;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN         1    269       Modification methylase LlaDCHIB.
FT                                /FTId=PRO_0000087954.
SQ   SEQUENCE   269 AA;  30925 MW;  0AB61895696B46EA CRC64;
     MAINEYKYGG VLMTKPYYEK ENAILVHADS FKLLEKIKPE SMDMIFADPP YFLSNGGMSN
     SGGQIVSVDK GDWDKISSFE EKHDFNRRWI RLARLVLKPN GTIWVSGSLH NIYSVGMALE
     QEGFKILNNI TWQKTNPAPN LSCRYFTHST ETILWARKND KKSRHYYNYE LMKEFNDGKQ
     MKDVWTGSLT KKSEKWAGKH PTQKPEYILE RIILASTKEN DYILDPFVGS GTTGVVAKRL
     GRKFIGIDSE KEYLKIAKKR LNKGATYGL
//
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