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Database: UniProt
Entry: P51892
LinkDB: P51892
Original site: P51892 
ID   DNLI1_XENLA             Reviewed;        1070 AA.
AC   P51892;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   10-MAY-2017, entry version 92.
DE   RecName: Full=DNA ligase 1;
DE            EC=6.5.1.1 {ECO:0000255|PROSITE-ProRule:PRU10135};
DE   AltName: Full=DNA ligase I;
DE   AltName: Full=Polydeoxyribonucleotide synthase [ATP] 1;
GN   Name=lig1;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus;
OC   Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8682316; DOI=10.1016/0378-1119(96)00175-8;
RA   Lepetit D., Thiebaud P., Aoufouchi S., Prigent C., Guesne R.,
RA   Theze N.;
RT   "The cloning and characterization of a cDNA encoding Xenopus laevis
RT   DNA ligase I.";
RL   Gene 172:273-277(1996).
CC   -!- FUNCTION: DNA ligase that seals nicks in double-stranded DNA
CC       during DNA replication, DNA recombination and DNA repair.
CC   -!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n)-3'-hydroxyl +
CC       5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) +
CC       AMP + diphosphate. {ECO:0000255|PROSITE-ProRule:PRU10135}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
CC       {ECO:0000305}.
DR   EMBL; L43496; AAB37754.1; -; mRNA.
DR   PIR; JC4852; JC4852.
DR   RefSeq; NP_001081653.1; NM_001088184.1.
DR   UniGene; Xl.78; -.
DR   ProteinModelPortal; P51892; -.
DR   SMR; P51892; -.
DR   PRIDE; P51892; -.
DR   GeneID; 397978; -.
DR   KEGG; xla:397978; -.
DR   CTD; 397978; -.
DR   Xenbase; XB-GENE-1009783; lig1.
DR   HOVERGEN; HBG005514; -.
DR   KO; K10747; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0071897; P:DNA biosynthetic process; IEA:InterPro.
DR   GO; GO:0051103; P:DNA ligation involved in DNA repair; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.3260.10; -; 1.
DR   InterPro; IPR000977; DNA_ligase_ATP-dep.
DR   InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR   InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR   InterPro; IPR016059; DNA_ligase_ATP-dep_CS.
DR   InterPro; IPR012308; DNA_ligase_ATP-dep_N.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Pfam; PF04679; DNA_ligase_A_C; 1.
DR   Pfam; PF01068; DNA_ligase_A_M; 1.
DR   Pfam; PF04675; DNA_ligase_A_N; 1.
DR   SUPFAM; SSF117018; SSF117018; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00574; dnl1; 1.
DR   PROSITE; PS00697; DNA_LIGASE_A1; 1.
DR   PROSITE; PS00333; DNA_LIGASE_A2; 1.
DR   PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cell cycle; Cell division; DNA damage; DNA recombination;
KW   DNA repair; DNA replication; Ligase; Magnesium; Metal-binding;
KW   Nucleotide-binding; Nucleus.
FT   CHAIN         1   1070       DNA ligase 1.
FT                                /FTId=PRO_0000059573.
FT   REGION      602    611       Interaction with target DNA.
FT                                {ECO:0000250}.
FT   REGION      795    797       Interaction with target DNA.
FT                                {ECO:0000250}.
FT   ACT_SITE    721    721       N6-AMP-lysine intermediate.
FT                                {ECO:0000255|PROSITE-ProRule:PRU10135}.
FT   METAL       774    774       Magnesium 1. {ECO:0000250}.
FT   METAL       873    873       Magnesium 2. {ECO:0000250}.
FT   BINDING     719    719       ATP. {ECO:0000250}.
FT   BINDING     726    726       ATP. {ECO:0000250}.
FT   BINDING     742    742       ATP. {ECO:0000250}.
FT   BINDING     878    878       ATP. {ECO:0000250}.
FT   BINDING     891    891       ATP. {ECO:0000250}.
FT   BINDING     897    897       ATP. {ECO:0000250}.
FT   SITE        458    458       Interaction with target DNA.
FT                                {ECO:0000250}.
FT   SITE        743    743       Interaction with target DNA.
FT                                {ECO:0000250}.
FT   SITE        923    923       Interaction with target DNA.
FT                                {ECO:0000250}.
FT   SITE        948    948       Interaction with target DNA.
FT                                {ECO:0000250}.
SQ   SEQUENCE   1070 AA;  120234 MW;  065D6E5B8C6E4E52 CRC64;
     MQRTIKSFFQ PKLGAEVKTK EEKVKNDVQK EKEEPEKSVP ERPLKERNGR ALCGDTESPV
     KRVSKKSARV LESDSEEEEE NSKVQATPEK MNNESDPKND VKETPPSARK ETPPSARKET
     PPSARKETPQ SARKETPPSA RKDAKSEGAS EMNTSQDFVS PCSSKSIDSP LCSDSPGISP
     SGIPKRKTAR KQLPKRKLEI SPSESNPPKD DFDVKGAVKR QKKEAEGDIQ QEEPMETSHN
     ISMEEECPIK KETNPDVVQE TISNADDPNI ELVDQKDVVS GEKQSEPKCQ EQPQPKLTSP
     TVEPKASKGK ARKKNSPLPK KVKFSDKVSF KDNDSEEKSN EEKSDEEPQK KADSAKPVKQ
     ISSFFAPRKP AIKTEKREEN LNEKNVTETS LEESPKPKKN VGSFFGASKQ EATEEQTEYN
     PSKSKYHPID DACWCNGQKV PYLAVARTFE RIEEESARLK NVETLSNFLR SVIALTPEDL
     LPCIYLCLNR LGPAYEGLEL GIGETILMKA VAQATGRQLE KIKAEAQEKG DLGLVAESSR
     SNQRTMFTPP KLMASGVFGK LKDIARMTGN ASMNKKIDII KGLFVACRHS EARYIARSLG
     GKLRIGLAEQ SVLSSIAQAV CLTPPGRDAP PTVMDAGKGM SADARKSWIE EKAMILKQTF
     CELPNYDAII PILLEHGIDD LPKHCRLTPG IPLKPMLAHP TKGIGEVLKR FDEAAFTCEY
     KYDGERAQIH ILENGEVHVY SRNQENNTTK YPDIISRIPK IKKESVKSCI LDTEAVAGDA
     EKKQIQPFQV LTTRKRKDVD ASEIKVQVCV YAFDMLYLNG ESLVKEPFAK RRQLLRDSFL
     ETEGQFMFAT YMDKSNTDEI SEFLDQSIKD SCEGLMVKTL EQDATYEIAK RSHNWLKLKK
     DYLEGVGDTL DLVVIGAYLG KGKRTGIYGG FLLASYDEES EEYQTICKIG TGFTDDDLEK
     HYNFLKDHVI ENPRSYYRWD SATEPDHWFD PVQVWEVKCA DLSISPVHKA ALGLVEDQKG
     ISLRFPRFLR IRDDKKPEES TNSFQVADLY RKQQQIQNTS STEKAEEDFY
//
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