ID UNC30_CAEEL Reviewed; 323 AA.
AC P52906; Q23021; Q7JLL9;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 2.
DT 27-MAR-2024, entry version 160.
DE RecName: Full=Homeobox protein unc-30;
DE AltName: Full=Uncoordinated protein 30;
GN Name=unc-30; ORFNames=B0564.10;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RC STRAIN=Bristol N2;
RX PubMed=7997265; DOI=10.1038/372780a0;
RA Jin Y., Hoskins R., Horvitz H.R.;
RT "Control of type-D GABAergic neuron differentiation by C. elegans UNC-30
RT homeodomain protein.";
RL Nature 372:780-783(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15882588; DOI=10.1016/j.ydbio.2005.01.032;
RA Shan G., Kim K., Li C., Walthall W.W.;
RT "Convergent genetic programs regulate similarities and differences between
RT related motor neuron classes in Caenorhabditis elegans.";
RL Dev. Biol. 280:494-503(2005).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=26083757; DOI=10.1038/nature14545;
RA Howell K., White J.G., Hobert O.;
RT "Spatiotemporal control of a novel synaptic organizer molecule.";
RL Nature 523:83-87(2015).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=29033363; DOI=10.1016/j.devcel.2017.09.013;
RA Yu B., Wang X., Wei S., Fu T., Dzakah E.E., Waqas A., Walthall W.W.,
RA Shan G.;
RT "Convergent Transcriptional Programs Regulate cAMP Levels in C. elegans
RT GABAergic Motor Neurons.";
RL Dev. Cell 43:212-226(2017).
CC -!- FUNCTION: Transcriptional regulator within D-type neurons that controls
CC terminal differentiation (PubMed:7997265). Required for the development
CC and function of the 19 inhibitory GABAergic (gamma-aminobutyric-acid-
CC secreting) D-type motor neurons, which control locomotion
CC (PubMed:7997265). Plays a role in regulating synapse formation in
CC dorsal D (DD) and ventral D (VD) GABAergic motor neurons, possibly in
CC part by regulating the expression of the neural regulator oig-1
CC (PubMed:26083757). Also required for expression of flp-13 in DD motor
CC neurons (PubMed:15882588). Plays a role in respecification of DD motor
CC neurons through regulation of genes involved in the modulation of
CC cellular cAMP including pde-4 which hydrolyzes cAMP and acy-1 which
CC catalyzes cAMP formation (PubMed:29033363).
CC {ECO:0000269|PubMed:15882588, ECO:0000269|PubMed:26083757,
CC ECO:0000269|PubMed:29033363, ECO:0000269|PubMed:7997265}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000269|PubMed:7997265}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b;
CC IsoId=P52906-1; Sequence=Displayed;
CC Name=a;
CC IsoId=P52906-2; Sequence=VSP_020103;
CC -!- TISSUE SPECIFICITY: Expressed in D-type GABAergic motor neurons at high
CC levels in young larvae, in which the motor circuitry is formed, and at
CC low levels in older animals (PubMed:7997265). Also present in six non-
CC GABAergic motor neurons and is absent from the seven non-D-type
CC GABAergic neurons (PubMed:7997265). Expressed in DD GABAergic motor
CC neurons and VD GABAergic motor neurons (PubMed:15882588,
CC PubMed:26083757, PubMed:29033363). {ECO:0000269|PubMed:15882588,
CC ECO:0000269|PubMed:26083757, ECO:0000269|PubMed:29033363,
CC ECO:0000269|PubMed:7997265}.
CC -!- DISRUPTION PHENOTYPE: Aberrant synaptic connectivity of dorsal and
CC ventral D-type motor neurons onto ventral and dorsal muscle.
CC {ECO:0000269|PubMed:26083757}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC {ECO:0000305}.
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DR EMBL; L37867; AAA63155.1; -; mRNA.
DR EMBL; Z73422; CAA97770.1; -; Genomic_DNA.
DR EMBL; Z73422; CAE54882.1; -; Genomic_DNA.
DR PIR; T18786; T18786.
DR PIR; T43635; T43635.
DR RefSeq; NP_001021276.1; NM_001026105.2. [P52906-2]
DR RefSeq; NP_001021277.1; NM_001026106.1. [P52906-1]
DR AlphaFoldDB; P52906; -.
DR SMR; P52906; -.
DR BioGRID; 43356; 4.
DR IntAct; P52906; 4.
DR STRING; 6239.B0564.10b.1; -.
DR PaxDb; 6239-B0564-10b; -.
DR EnsemblMetazoa; B0564.10a.1; B0564.10a.1; WBGene00006766. [P52906-2]
DR EnsemblMetazoa; B0564.10b.1; B0564.10b.1; WBGene00006766. [P52906-1]
DR GeneID; 178265; -.
DR KEGG; cel:CELE_B0564.10; -.
DR UCSC; B0564.10a; c. elegans. [P52906-1]
DR AGR; WB:WBGene00006766; -.
DR WormBase; B0564.10a; CE05183; WBGene00006766; unc-30. [P52906-2]
DR WormBase; B0564.10b; CE36087; WBGene00006766; unc-30. [P52906-1]
DR eggNOG; KOG0486; Eukaryota.
DR GeneTree; ENSGT00940000168132; -.
DR InParanoid; P52906; -.
DR OMA; FNWQIKP; -.
DR OrthoDB; 5395268at2759; -.
DR PhylomeDB; P52906; -.
DR PRO; PR:P52906; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00006766; Expressed in pharyngeal muscle cell (C elegans) and 3 other cell types or tissues.
DR ExpressionAtlas; P52906; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0007411; P:axon guidance; IMP:UniProtKB.
DR GO; GO:0033563; P:dorsal/ventral axon guidance; IMP:WormBase.
DR GO; GO:0097154; P:GABAergic neuron differentiation; IMP:WormBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:WormBase.
DR GO; GO:0040012; P:regulation of locomotion; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR016233; Homeobox_Pitx/unc30.
DR PANTHER; PTHR45882; PITUITARY HOMEOBOX HOMOLOG PTX1; 1.
DR PANTHER; PTHR45882:SF3; PITUITARY HOMEOBOX HOMOLOG PTX1; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR PIRSF; PIRSF000563; Homeobox_protein_Pitx/Unc30; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; DNA-binding; Homeobox;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..323
FT /note="Homeobox protein unc-30"
FT /id="PRO_0000049346"
FT DNA_BIND 112..171
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 83..119
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 92..110
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 251..254
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000303|PubMed:7997265"
FT /id="VSP_020103"
FT CONFLICT 39..40
FT /note="EH -> DD (in Ref. 1; AAA63155)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 323 AA; 36202 MW; 9F76008ECA259A7A CRC64;
MDDNTATLTA IHQQQQSHNR FSNPLVCVGQ LDHHSLLPEH SISSSLAPLT HNPYAFNYSI
PLPPTDITTK LPKLELLSLD VKQEQDDNHL DTSSPTDSTG NGSTNGGKIQ KPRRQRTHFT
SHQLTELENW FSRNRYPDMA CREEIAVWIS LTEPRVRVWF KNRRAKWRKR ERNYVIDNGQ
GTTKVTAQSL DPLGSLQNTF PQTLLQSSSS QLDDSAVTSS SFYGYGGAWQ QNPYYSRNNQ
TTFNWQIKPQ DQFQFQTIPM SPTTATSRFS TAANLAPLPT AQAAFSTSAT SSNDKLKLMD
GLSNSLSSSL GQPYQPCQYS GPL
//
