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Database: UniProt
Entry: P52985
LinkDB: P52985
Original site: P52985 
ID   DHOM_LACLC              Reviewed;         428 AA.
AC   P52985;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   05-JUL-2017, entry version 83.
DE   RecName: Full=Homoserine dehydrogenase;
DE            Short=HDH;
DE            EC=1.1.1.3;
GN   Name=hom;
OS   Lactococcus lactis subsp. cremoris (Streptococcus cremoris).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=1359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC   STRAIN=MG1614;
RX   PubMed=8682767;
RA   Madsen S.M., Albrechtsen B., Hansen E.B., Israelsen H.;
RT   "Cloning and transcriptional analysis of two threonine biosynthetic
RT   genes from Lactococcus lactis MG1614.";
RL   J. Bacteriol. 178:3689-3694(1996).
CC   -!- CATALYTIC ACTIVITY: L-homoserine + NAD(P)(+) = L-aspartate 4-
CC       semialdehyde + NAD(P)H.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de
CC       novo pathway; L-homoserine from L-aspartate: step 3/3.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L-
CC       threonine from L-aspartate: step 3/5.
CC   -!- DISRUPTION PHENOTYPE: Mutant containing a deletion in both hom and
CC       thrB is unable to grow in a defined medium lacking threonine.
CC       {ECO:0000269|PubMed:8682767}.
CC   -!- SIMILARITY: Belongs to the homoserine dehydrogenase family.
CC       {ECO:0000305}.
DR   EMBL; X96988; CAA65713.1; -; Genomic_DNA.
DR   PIR; JC6049; JC6049.
DR   RefSeq; WP_011835205.1; NZ_LITG01000003.1.
DR   ProteinModelPortal; P52985; -.
DR   SMR; P52985; -.
DR   eggNOG; ENOG4105D6E; Bacteria.
DR   eggNOG; COG0460; LUCA.
DR   UniPathway; UPA00050; UER00063.
DR   UniPathway; UPA00051; UER00465.
DR   GO; GO:0004412; F:homoserine dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0009088; P:threonine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR002912; ACT_dom.
DR   InterPro; IPR005106; Asp/hSer_DH_NAD-bd.
DR   InterPro; IPR016204; HDH.
DR   InterPro; IPR001342; HDH_cat.
DR   InterPro; IPR019811; HDH_CS.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Pfam; PF00742; Homoserine_dh; 1.
DR   Pfam; PF03447; NAD_binding_3; 1.
DR   PIRSF; PIRSF000098; Homoser_dehydrog; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS51671; ACT; 1.
DR   PROSITE; PS01042; HOMOSER_DHGENASE; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Isoleucine biosynthesis; Methionine biosynthesis; NADP;
KW   Oxidoreductase; Threonine biosynthesis.
FT   CHAIN         1    428       Homoserine dehydrogenase.
FT                                /FTId=PRO_0000066697.
FT   DOMAIN      351    425       ACT. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01007}.
FT   NP_BIND       8     15       NADP. {ECO:0000250}.
FT   ACT_SITE    206    206       Proton donor. {ECO:0000255}.
FT   BINDING     106    106       NADP. {ECO:0000250}.
FT   BINDING     191    191       Substrate. {ECO:0000250}.
SQ   SEQUENCE   428 AA;  46629 MW;  B178CF30D62A6349 CRC64;
     MAVNIAILGF GTVGTGLPTL LSENKEKLAK ILDEEIVISK VLMRDNKAIE KARSQGFNYD
     FVLNLDDILA DSEISIVVEL MGRIEPAKTY ITQAIEAGKN VVTANKDLLA VHGVELRSLA
     QKHHVALYYE AAVAGGIPIL RTLANSFSSD KITHLLGILN GTSNFMMTKM SEEGWTYDES
     LAKAQELGYA ESDPTNDVDG IDASYKLAIL SEFAFGMTLA PDDIAKSGLR SIQKTDVEIA
     QQFGYVLKLT GEINEVDSGI FAEVSPTFLP KSHPLASVNG VMNAVFIESE GIGDSVFYGA
     GAGQKPTATS VLADIVRIVK RVKDGTIGKS FNEYARSTSL ANPHDIENKY YFSVETPDST
     GQLLLLVELF TSENVSFEQV LQQKGNGKRA VVVIISHKIN RVQLSAIQDK LNQEKDFKLL
     NRFKVLGD
//
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